Investigation on endosymbionts of Mediterranean flour moth gut and studying their role in physiology and biology

Goharrostami, Maryam; Sendi, Jalal Jalali

doi:10.1016/j.jspr.2017.11.003

Cited by 3 publications

(3 citation statements)

References 36 publications

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“…Certain bacterial populations may persist throughout the life cycle, despite extensive gut changes during pupation and metamorphosis 19 , 23 . Despite the absence of specialized gut structures and rapid food transition 24 , bacteria may form a biofilm, suggesting that they may have an ability to colonize the gut 25 . Therefore, the caterpillar microbiome is likely a multilayer system comprising core taxa and a more flexible non-core microbiome 26 , 27 which has a controversial functional role.…”

Section: Introductionmentioning

confidence: 99%

Fungi are more transient than bacteria in caterpillar gut microbiomes

Šigut

Pyszko

Šigutová

et al. 2022

Sci Rep

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Despite an increasing number of studies on caterpillar (Insecta: Lepidoptera) gut microbiota, bacteria have been emphasized more than fungi. Therefore, we lack data on whether fungal microbiota is resident or transient and shaped by factors similar to those of bacteria. We sampled nine polyphagous caterpillar species from several tree species at multiple sites to determine the factors shaping leaf and gut bacterial and fungal microbiota as well as the extent to which caterpillars acquire microbiota from their diet. We performed 16S and ITS2 DNA metabarcoding of the leaves and guts to determine the composition and richness of the respective microbiota. While spatial variables shaped the bacterial and fungal microbiota of the leaves, they only affected fungi in the guts, whereas the bacteria were shaped primarily by caterpillar species, with some species harboring more specific bacterial consortia. Leaf and gut microbiota significantly differed; in bacteria, this difference was more pronounced. The quantitative similarity between leaves and guts significantly differed among caterpillar species in bacteria but not fungi, suggesting that some species have more transient bacterial microbiota. Our results suggest the complexity of the factors shaping the gut microbiota, while highlighting interspecific differences in microbiota residency within the same insect functional group.

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Section: Introductionmentioning

confidence: 99%

Fungi are more transient than bacteria in caterpillar gut microbiomes

Šigut

Pyszko

Šigutová

et al. 2022

Sci Rep

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“…Однако мы регистрировали высокий уровень пищеварительных альфа-амилаз у насекомых, получающих диету с антибиотиками в десятой дочерней генерации. В исследовании Goharrostami et al [2018] воздействие тетрациклином также приводило к увеличению активности пищеварения у мельничной огнёвки Ephestia kuehniella и авторы связали данный эффект с недостаточным количеством кишечных бактерий у хозяина. Данное исследование ограничивалось лишь первой генерацией насекомых.…”

Section: Discussionunclassified

Effect of an antibiotic on gut microbiota and activity of digestive and antioxidant enzymes of Galleria mellonella

2022

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Резюме. Широкое использование антибиотических веществ приводит к появлению в окружающей среде большого количества антибиотикорезистентных микроорганизмов, непосредственно самих антибиотиков и продуктов их распада, что сопровождается формированием мультирезистентных форм бактерий в окружающей среде, в том числе в кишечнике различных животных, и их успешной адаптации в различных экологических условиях. В течение десяти поколений вощиной огнёвки Galleria mellonella было изучено влияние антибиотика (амикацин) на микробиоту среднего кишечника личинок, а также уровни активности антиоксидантных и пищеварительных ферментов. В десятой генерации огнёвки на диете с антибиотиком количество бактерий в кишечнике личинок восстановилось до значений контрольной группы. Кроме того, в десятой генерации наблюдалось двухкратное увеличение активности пищеварительных ферментов и восстановление активности антиоксидантных ферментов до значений в контрольной группе. Зарегистрированные изменения в активности пищеварительных ферментов и ферментов антиоксидантной системы можно рассматривать как адаптационный механизм воздействия антибиотических веществ как на клетки организма хозяина, так и клетки микроорганизмов, составляющих микробиоту.

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“…Moreover, certain bacterial populations may persist throughout the life cycle, despite extensive gut changes during pupation and metamorphosis 19,23 . Furthermore, despite the absence of specialized gut structures and rapid food transition 24 , bacteria may form a bio lm, suggesting their ability to colonize the gut 25 . Thus, the caterpillar microbiome is likely a multilayer system composed of core taxa and a more exible non-core microbiome 26,27 with a controversial functional role.…”

Section: Introductionmentioning

confidence: 99%

Caterpillar Gut Microbiome: Fungi Are More Transient Than Bacteria

Šigut

Pyszko

Šigutová

et al. 2022

Preprint

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Despite increasing studies on caterpillar (Insecta: Lepidoptera) gut microbiota, bacteria have been emphasized more than fungi. Therefore, we lack data on whether fungal microbiota is resident or transient and shaped by factors similar to those of bacteria. We sampled nine polyphagous caterpillar species from several tree species at multiple sites to determine the factors shaping leaf and gut bacterial and fungal microbiota as well as the extent to which caterpillars acquire microbiota from their diet. We performed 16S and ITS2 DNA metabarcoding of leaves and guts to determine the composition and richness of respective microbiota. While spatial variables shaped both the bacterial and fungal microbiota of the leaves, they only affected fungi in the guts, whereas bacteria were shaped primarily by caterpillar species, with some species harboring more specific bacterial consortia. Leaf and gut microbiota significantly differed; in bacteria, this difference was much more pronounced. Moreover, the leaf–gut similarity of bacterial but not fungal components differed significantly among caterpillar species, suggesting that some species have more transient bacterial microbiota than others. Our results suggest the complexity of the factors shaping the gut microbiota, while pointing out interspecific differences in microbiota residency within the same insect functional group.

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Investigation on endosymbionts of Mediterranean flour moth gut and studying their role in physiology and biology

Cited by 3 publications

References 36 publications

Fungi are more transient than bacteria in caterpillar gut microbiomes

Fungi are more transient than bacteria in caterpillar gut microbiomes

Effect of an antibiotic on gut microbiota and activity of digestive and antioxidant enzymes of Galleria mellonella

Caterpillar Gut Microbiome: Fungi Are More Transient Than Bacteria

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