Investigation on salt-response mechanisms in<i>Arabidopsis thaliana</i>from UniProt protein knowledgebase

Guo, Meili; Liu, Xin; Wang, Jiahui; Li, Lei; Zhang, Wendi; Gong, Benjiao; Zhang, Chenglin; Zhou, Chunxi

doi:10.1080/17429145.2018.1551581

Cited by 14 publications

(15 citation statements)

References 31 publications

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“…The differential induction of genes involved in salt and osmotic stress in BG83, such as aspartic peptidase domain-containing proteins (Pa_contig06808, Pa_contig04419, Pa_contig02817, Pa_contig05393) [30], gdsl esterase lipase protein (Pa_contig00520) [31] and btb poz and taz domain-containing protein (Pa_contig00582) [32], could indicate that R . necatrix penetration into the roots is associated with osmotic effects, suggesting that BG83’s tolerance to R .…”

Section: Resultsmentioning

confidence: 99%

Rosellinia necatrix infection induces differential gene expression between tolerant and susceptible avocado rootstocks

et al. 2019

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Rosellinia necatrix is the causal agent of avocado white root rot (WRR). Control of this soil-borne disease is difficult, and the use of tolerant rootstocks may present an effective method to lessen its impact. To date, no studies on the molecular mechanisms regulating the avocado plant response towards this pathogen have been undertaken. To shed light on the mechanisms underpinning disease susceptibility and tolerance, molecular analysis of the gene’s response in two avocado rootstocks with a contrasting disease reaction was assessed. Gene expression profiles against R . necatrix were carried out in the susceptible ‘Dusa’ and the tolerant selection BG83 avocado genotypes by micro-array analysis. In ‘Dusa’, the early response was mainly related to redox processes and cell-wall degradation activities, all becoming enhanced after disease progression affected photosynthetic capacity, whereas tolerance to R . necatrix in BG83 relied on the induction of protease inhibitors and their negative regulators, as well as genes related to tolerance to salt and osmotic stress such as aspartic peptidase domain-containing proteins and gdsl esterase lipase proteins. In addition, three protease inhibitors were identified, glu protease, trypsin and endopeptidase inhibitors, which were highly overexpressed in the tolerant genotype when compared to susceptible ‘Dusa’, after infection with R . necatrix , reaching fold change values of 52, 19 and 38, respectively. The contrasting results between ‘Dusa’ and BG83 provide new insights into the different mechanisms involved in avocado tolerance to Phytophthora cinnamomi and R . necatrix , which are consistent with their biotrophic and necrotrophic lifestyles, respectively. The differential induction of genes involved in salt and osmotic stress in BG83 could indicate that R . necatrix penetration into the roots is associated with osmotic effects, suggesting that BG83’s tolerance to R . necatrix is related to the ability to withstand osmotic imbalance. In addition, the high expression of protease inhibitors in tolerant BG83 compared to susceptible ‘Dusa’ after infection with the pathogen suggests the important role that these proteins may play in the defence of avocado rootstocks against R . necatrix .

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Section: Resultsmentioning

confidence: 99%

Rosellinia necatrix infection induces differential gene expression between tolerant and susceptible avocado rootstocks

et al. 2019

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“…A detailed analysis of 466 salts responsive A. thaliana proteins retrieved from UniPort and analysed by bioinformatics tools like PANTHER, DAVID, KEGG etc. correlated the involvement of salt responsive proteins both in abiotic and several biotic conditions (43). A presence of protein (26 kDa band), linked with salt tolerance in rice was reported by Rani and Reddy (1994) (44).…”

Section: Superoxide Dismutasementioning

confidence: 61%

Evaluation of proteome complexes normalizing osmoregulation in salt stressed Luffa acutangula (L.) Roxb.

Pramodkumar

Singh

et al. 2021

Plant Sci. Today

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Modern-day agriculture is facing the challenge of sustaining global food security. However, the rapid increase in salinity stress among arable areas poses a major threat to crop health and yield. Salinity stress is one of the most common and rapidly spreading stress that has a detrimental effect on the productivity of edible plant family i.e. Cucurbitaceae. The present study endeavors to evaluate the Osmoregulators (anti-oxidants and proteins), that supports the growth of two varieties of Luffa acutangula (L.) Roxb. under salt stress. The 2-3 weeks old saplings were exposed to salt stress (up to 200 mM NaCl) for one week. Post-treatment the osmoregulatory metabolites like Trehalose, Proline & enzymic anti-oxidants like peroxidase (POD), Superoxide dismutase (SOD) and proteins using LC-MS/MS were analyzed. In both the varieties, Trehalose increased with increasing salt concentration, while the level of Proline increased in Variety 1 and decreased in Variety 2. With increasing salt concentrations, the POD activity decreased in both varieties whereas that of SOD levels increased in Variety 2 and decreased in Variety 1. The protein identified by LC-MS/MS and functional annotation analysis employing Uniport database & BlastP algorithm, aided in the detection of differentially expressed proteins in response to salt stress. This was followed by metabolic interaction annotation enrichment analysis by FunRich 3.0 tool, enabling characterization of proteins to be involved in the Calvin cycle, amino acids biosynthesis, carbohydrate and energy metabolism, ROS defence, hormonal biosynthesis and signal transduction. The augmentation of the metabolic activities of the Calvin cycle, biosynthesis of amino acids, carotenoids and peroxisomes, glycolytic pathway and the tricarboxylic acid cycle will conceivably influence the photosynthetic capacity in L. acutangula varieties under salt stress. The upsurge of key enzymes involved in these above described biological processes possibly appears to play an important role in the enhancement of salt tolerance.

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“…The ICE-CBF-COR pathway is an important signaling pathway associated with low-temperature stress (Guo et al 2019b;Jin et al 2018). In the present study, the CBFs, inducer of CBF expression (ICEs), and cold-responsive (COR) genes were differentially expressed under cold stress.…”

Section: Discussionmentioning

confidence: 99%

Identification Of Arabidopsis genes associated with cold tolerance based on integrated bioinformatics analysis

Guo¹,

Liu

Jiang³

et al. 2021

Journal of Plant Interactions

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Cold stress is a major environmental factor that limits plant growth and productivity. Plants have evolved various strategies to adapt to these environmental conditions. To better explain the mechanisms used to survive environmental challenges, we retrieved the cold-responsive genes of Arabidopsis thaliana from the Gene Expression Omnibus (GEO) database. The GEO raw data were normalized by the quantile method, and then the differentially expressed genes (DEGs) under cold stress were screened using the robust rank aggregation (RRA) algorithm, including 261 upregulated and 177 downregulated genes out of more than 20,000 genes. Further, the integrated bioinformatics analyses of PUBMED, PANTHER, DAVID, and STRING indicated that the upregulated DEGs were involved in cellular response to red light, negative regulation of circadian rhythm, photoprotection, monosaccharide transport, cold acclimation, and phosphate ion homeostasis, while the downregulated DEGs were associated with the indole glucosinolate biosynthetic process, regulation of RNA splicing, water transport, cell wall modification, cell wall loosening, cellular water homeostasis, and cell wall homeostasis. Furthermore, the up-regulated DEGs had about four times protein-protein-interactions (PPIs) than the down-regulated DEGs, and the cold-responsive genes were identified using Cytoscape software. Furthermore, qRT-PCR of low-temperatureresponsive protein 78 (LTI78), transducin family protein (SWA1), and arginine methyltransferase 11 (PRMT11) were performed to validate the outcome of integrated bioinformatics analysis. Our work will improve our knowledge of cold-responsive mechanisms and these DEGs might be targets for plant cold stress-resistance research.

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Investigation on salt-response mechanisms inArabidopsis thalianafrom UniProt protein knowledgebase

Cited by 14 publications

References 31 publications

Rosellinia necatrix infection induces differential gene expression between tolerant and susceptible avocado rootstocks

Rosellinia necatrix infection induces differential gene expression between tolerant and susceptible avocado rootstocks

Evaluation of proteome complexes normalizing osmoregulation in salt stressed Luffa acutangula (L.) Roxb.

Identification Of Arabidopsis genes associated with cold tolerance based on integrated bioinformatics analysis

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