Investigation on Species Authenticity for Herbal Products of Celastrus Orbiculatus and Tripterygum Wilfordii from Markets Using ITS2 Barcoding

Abstract

Herbal material is both a medicine and a commodity. Accurate identification of herbal materials is necessary to ensure the safety and effectiveness of medication. With this work, we initiated an identification method to investigate the species authenticity for herbal products of Celastrus orbiculatus and Tripterygum wilfordii utilizing DNA barcoding technology. An ITS2 (internal transcribed spacer two) barcode database including 59 sequences was successfully established to estimate the reliability of species-level identification for Celastrus and Tripterygium. Our findings showed that ITS2 can effectively and clearly distinguish C. orbiculatus, T. wilfordii and its congeners. Then, we investigated the proportions and varieties of adulterant species in the herbal markets. The data from ITS2 region indicated that 13 (62%) of the 21 samples labeled as “Nan-she-teng” and eight (31%) of the 26 samples labeled as “Lei-gong-teng” were authentic; the remaining were adulterants. Of the 47 herbal products, approximately 55% of the product identity were not in accordance with the label. In summary, we support the efficacy of the ITS2 barcode for the traceability of C. orbiculatus and T. wilfordii, and the present study provides one method and reference for the identification of the herbal materials and adulterants in the medicinal markets.

Specification and DNA Barcoding of Thai Traditional Remedy for Chronic Kidney Disease: Pikad Tri-phol-sa-mut-than

“…This is because they were found to contain short sequences (approximately 200–230 bp) [ 13 , 38 , 39 ]. Therefore, ITS2 was identified as a good barcode for the purposes of identification and authentication of herbal medicines used as raw materials, processed materials, and finished products [ 39 , 40 , 41 , 42 ]. Additionally, many research articles have reported the need to integrate trnH-psbA and ITS2 regions for plant species identification [ 43 , 44 , 45 , 46 ].…”

Development of a Genus-Universal Nucleotide Signature for the Identification and Supervision of Ephedra-Containing Products

“…Han et al investigated 1436 samples representing 295 medicinal species from seven herb markets in China, and found that 1260 samples were successfully identified by ITS2 barcodes, among which approximately 4.2% were adulterants [ 29 ]. The survey of species authenticity for herbal products using DNA barcoding showed that only 62% of the 21 samples labeled as “Nan-she-teng ( Celastrus orbiculatus )” and 31% of the 26 samples labeled as “Lei-gong-teng ( Tripterygium wilfordii )” were genuine [ 30 ]. However, in view of the strict regulation, to avoid inspection authorities, the Ephedra raw materials are often transported and traded by lawbreakers in the forms of powder, crude extract, compound preparations, and other processed products.…”

Integrated Approach for Species Identification and Quality Analysis for Labisia pumila Using DNA Barcoding and HPLC

“…This was also used to calculate the concentration of the standards in the plant extracts. The peak areas corresponding to gallic acid and rutin were assigned by comparing their retention times with those of the reference standard [60].…”