Investigation on ¹O₂ generation ability of di‐sulfonic phthalocyanine zinc isomers using an HPLC–CL system

Abstract

An HPLC system combining a chemiluminescence detector was applied to estimate the singlet oxygen ((1)O2) generation ability of di-sulfonic phthalocyanine zinc (ZnPcS2 ) isomers. As photosensitizers, ZnPcS2 produces (1)O2 in air-saturated solutions under photoirradiation. The latter reacts with methyl Cypridina luciferin analogue (MCLA) to initiate chemiluminescence. This photoinduced chemiluminescence (PCL) of MCLA provides an easy method for evaluating the isomers' (1)O2 generation ability during a simultaneous HPLC separation procedure. The cis-isomers and trans-isomers of ZnPcS2 show different (1)O2 generation abilities, which are in accordance with differences in their absorption spectra.

Mechanistic Insight into Marine Bioluminescence: Photochemistry of the Chemiexcited Cypridina (Sea Firefly) Lumophore

“…The BL reaction requires only three components: luciferin, luciferase, and oxygen. Unlike many other endocellular BL reactions, additional species such as ATP, magnesium ions, or calcium ions are not required in the Cypridina BL, which could be advantageous for some intended applications. ,, Together with the high BL efficiency (Φ BL = 0.28), , this turns the Cypridina BL into a valuable detection system for active oxygen species − in biological systems. Cypridina BL has already become an indispensable analytical tool in biotechnology and biomedical research, such as enzyme immunoassays, − imaging, − quantum-dot-assay-based bioluminescence resonance energy transfer (BRET), immunohistology (IHC), spectroscopy with two-photon fluorescent probes, and monitoring of promoter activity without destroying the cells or tissues. − …”