Investigation on the Incidence, Diagnostic, Possibilities and Design of Prophylactic Treatment in an Outbreak of Bacterial Haemorrhagic Septicemia of Carp

Abstract

The investigations carried out on a sample of 53 breeders (31 females and 22 males),in the aquaculture farming system ofSuatu village, County of Cluj, concerning the incidence of bacterial hemorrhagicsepticaemia, its diagnosis and prophylactico-therapeutic possibilities. The results highlights an incidence of bacteriosis differentiated according to the category of breeders, 54.8% in females and36.3% in males. Necropsy examination carried out on 3 specimens of suspected bacteriosis, highlights the presence of hemorrhagic skin lesions, which are dotted or diffuse, respectivelymusculo-cutaneous necrosis and ulcers. Regardingthe internal injuries, opening of the body cavity, revealedconsistency changes and septicemy form in major internal organs, as well as the presence of a sanguinolent appearence liquid.The bacterioscopic examination of smears from the pathologic material (skin surface and internal samples) colored with the Gram method, reveled bacterial microflora, represented by coccobacillus and straight bacilli or slightly curved, both Gram negative. Furthermore, the bacteriological (culture) examination performedon usual and selective culture mediae, distinguish the isolation of pure culture bacteriae belonging to the genus Aeromonas.The exam of biochemical traits, with the aid of API 20 E multitest determines that the isolated strains of Aeromonas spp. belong to the species Aeromonas hydrophila, indicted in the emergence and evolution of the bacterial of haemorrhagic septicaemia.Testing strains sensitivity to antibiotics and chemotherapy (through antibiograma level), reflects the isolated bacteria's values of sensitivity in decreasing order: amoxicillin, oxytetracycline, florfenicoland enrofloxacilin.However, reduced sensitivity was recorded to tetracycline and ampicillin and resistance to erythromycin.