Investigation on the phase behaviour of gelatin/agarose mixture in an environment of reduced solvent quality

Almrhag, Omar; George, Paul; Банникова, Анна; Katopo, Lita; Chaudhary, Deeptangshu; Kasapis, Stefan

doi:10.1016/j.foodchem.2012.08.050

Cited by 7 publications

(2 citation statements)

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“…The same effect was observed in [35]. The observed spectral alterations in the FTIR spectra of native gelatin confirmed strong intermolecular interactions (electrostatic interactions and hydrogen bonding) between polypeptide chains of gelatin and macromolecules of polysaccharides during the self-organisation of polyelectrolyte complexes, which were also observed for complexes of gelatin with gellan [21,36], xanthan gum [37], agarose [38] and fully deacetylated chitosan [39].…”

Section: Resultssupporting

confidence: 67%

Polyelectrolyte Polysaccharide–Gelatin Complexes: Rheology and Structure

et al. 2020

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General features of rheological properties and structural peculiarities of polyelectrolyte polysaccharide–gelatin complexes were discussed in this paper. Experimental results were obtained for typical complexes, such as κ-carrageenan–gelatin, chitosan–gelatin and sodium alginate–gelatin complexes. A rheological method allows us to examine the physical state of a complex in aqueous phase and the kinetics of the sol–gel transition and temperature dependences of properties as a result of structural changes. The storage modulus below the gelation temperature is constant, which is a reflection of the solid-like state of a material. The gels of these complexes are usually viscoplastic media. The quantitative values of the rheological parameters depend on the ratio of the components in the complexes. The formation of the structure as a result of strong interactions of the components in the complexes was confirmed by UV and FTIR data and SEM analysis. Interaction with polysaccharides causes a change in the secondary structure of gelatin, i.e., the content of triple helices in an α-chain increases. The joint analysis of the structural and rheological characteristics suggests that the formation of additional junctions in the complex gel network results in increases in elasticity and hardening compared with those of the native gelatin.

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Section: Resultssupporting

confidence: 67%

Polyelectrolyte Polysaccharide–Gelatin Complexes: Rheology and Structure

et al. 2020

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“…Agarose, as a linear polysaccharide based on the 3,6-anhydro-α-L-galactopyranose unit, is originated from red seaweed. Agarose is natural, biocompatible and biodegradable and it has been widely used in food, cosmetic and pharmaceutical applications (16)(17)(18). The major difference between agarose and agar is that agar is composed of a mixture of agarose and agaropectin.…”

Section: Introductionmentioning

confidence: 99%

Non-toxic agarose/gelatin-based microencapsulation system containing gallic acid for antifungal application

Lam

Gambari

Kok

et al. 2014

International Journal of Molecular Medicine

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Abstract.Aspergillus niger (A. niger) is a common species of Aspergillus molds. Cutaneous aspergillosis usually occurs in skin sites near intravenous injection and approximately 6% of cutaneous aspergillosis cases which do not involve burn or HIV-infected patients are caused by A. niger. Biomaterials and biopharmaceuticals produced from microparticle-based drug delivery systems have received much attention as microencapsulated drugs offer an improvement in therapeutic efficacy due to better human absorption. The frequently used crosslinker, glutaraldehyde, in gelatin-based microencapsulation systems is considered harmful to human beings. In order to tackle the potential risks, agarose has become an alternative polymer to be used with gelatin as wall matrix materials of microcapsules. In the present study, we report the eco-friendly use of an agarose/gelatin-based microencapsulation system to enhance the antifungal activity of gallic acid and reduce its potential cytotoxic effects towards human skin keratinocytes. We used optimal parameter combinations, such as an agarose/gelatin ratio of 1:1, a polymer/oil ratio of 1:60, a surfactant volume of 1% w/w and a stirring speed of 900 rpm. The minimum inhibitory concentration of microencapsulated gallic acid (62.5 µg/ml) was significantly improved when compared with that of the original drug (>750 µg/ml). The anti-A. niger activity of gallic acid -containing microcapsules was much stronger than that of the original drug. Following 48 h of treatment, skin cell survival was approximately 90% with agarose/gelatin microcapsules containing gallic acid, whereas cell viability was only 25-35% with free gallic acid. Our results demonstrate that agarose/gelatin-based microcapsules containing gallic acid may prove to be helpful in the treatment of A. niger-induced skin infections near intravenous injection sites.

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