Investigation the<i>in vitro</i>biological performance of graphene/bioactive glass scaffolds using MC3T3-E1 and ATDC5 cells

Deliormanlı, Aylin M.; Türk, Mert

doi:10.1080/10667857.2018.1517994

Cited by 7 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Observed effects were attributed to the differences in bioactive glass ion release amounts and to potential effects of K + and Mg +2 ions from 13-93 bioactive glass. 44 Previous study of Deliormanlı et al 23 on graphene-containing, PCL coated, sponge-like 13-93 bioactive glass scaffolds revealed that the prepared composite scaffolds were not cytotoxic to the preosteoblastic MC3T3-E1 and chondrogenic ATDC5 cells (except for the samples containing 10 wt% graphene) and they proliferated well on the scaffolds. It is known that high concentrations of graphene can alter the integrity of the plasma membrane and induce cell death.…”

Section: Discussionmentioning

confidence: 93%

“…However, in the current study, it was shown that even in the presence of 10 wt% graphene nanoflakes a detrimental effect was not observed on the viability of the mBMSCs. This may be due to lower degradation rates of robocast bioactive glass scaffolds having struts with $400 mm and relatively lower porosity values (47%) compared to the sponge-like scaffolds prepared by polymer foam replication technique 23 (strut size $100 mm and porosity $66.8%).…”

Section: Discussionmentioning

confidence: 99%

“…In the past, these biomaterials were mainly investigated as composites in vitro, using pre-osteoblastic and chondrogenic cells. 23,24 In this study it was intended to investigate the effect of graphene nanopowders in the form of nanoflakes on the physical and the biological properties of grid-like bioactive glass scaffolds prepared by robocasting. For this purpose, mBMSCs were utilized and their viability, proliferation, and differentiation on the prepared composite scaffolds were investigated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Response of mouse bone marrow mesenchymal stem cells to graphene-containing grid-like bioactive glass scaffolds produced by robocasting

2018

Self Cite

View full text Add to dashboard Cite

In the study, three-dimensional, grid-like silicate-based bioactive glass scaffolds were manufactured using a robotic deposition technique. Inks were prepared by mixing 13-93 bioactive glass particles in Pluronic V R F-127 solution. After deposition, scaffolds were dried at room temperature and sintered at 690 C for 1 h. The surface of the sintered scaffolds was coated with graphene nanopowder (1, 3, 5, 10 wt%) containing poly(e-caprolactone) solution. The in vitro mineralization ability of the prepared composite scaffolds was investigated in simulated body fluid. The surface of the simulated body fluid-treated scaffolds was analyzed using scanning electron microscopy to investigate the hydroxyapatite formation. Mechanical properties were tested under compression. Results revealed that graphene coating has no detrimental effect on the hydroxyapatite forming ability of the prepared glass scaffolds. On the other hand, it decreased the compression strength of the scaffolds at high graphene concentrations. The prepared grid-like bioactive glass-based composite scaffolds did not show toxic response to bone marrow mesenchymal stem cells. It was shown that stem cells seeded onto the scaffolds attached and proliferated well on the surface. Cells seeded on the scaffolds surface also demonstrated osteogenic differentiation under in vitro conditions in the absence of transforming growth factors.

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Response of mouse bone marrow mesenchymal stem cells to graphene-containing grid-like bioactive glass scaffolds produced by robocasting

2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…The physical structure of zein-ICA/PLGA-MSC provided a suitable environment for the development of embedded MSC, MSC in zein-ICA/PLGA-MSC grew well and were arranged orderly, which is consistent with the results of fluorescence images. Although the scaffolds prepared by electrospinning have good compatibility with stem cells, most of them are used very limitedly to realize uniform distribution and directional growth of stem cells ( Deliormanlı et al, 2018 ; Sattary et al, 2022 ). Since the introduction of bioelectrospraying seeding method, the cell laden scaffold material has uniform cell arrangement and directional growth.…”

Section: Discussionmentioning

confidence: 99%

Three-dimensional mesenchymal stem cell laden scaffold of icariin sustained-release for bone regeneration

LIU¹,

FANG²

2022

Turkish Journal of Biology

View full text Add to dashboard Cite

Background/aim Icariin (ICA)-loaded zein/PLGA nanofiber membrane combined with MSCs was prepared by coaxial electrospinning and bioelectrospraying. Materials and methods SEM and TEM were used to evaluate the surface morphology and microstructure of the fiber membrane. Ultraviolet spectrophotometry was used to detect drug release. A LIVE/DEAD Viability/Cytotoxicity Kit and fluorescence staining were used to detect cell morphology and activity. Alkaline phosphatase and calcium mineralization deposition were used to evaluate the osteoinductive activity of the scaffold. Dynamic mechanical analysis was used to determine the Young’s modulus, maximum load, and maximum elongation of the prepared scaffold. Western blot was used to detect the related protein expression in MSCs induced by drug-loaded scaffolds. Results Good mechanical properties and stability were observed in the prepared drug-loaded scaffolds. SEM showed that there were a considerable number of MSCs dispersed in the scaffold. MSCs were evenly distributed, could grow evenly between fibers, and were arranged orderly along the fibers. Sustained release of ICA confers cell laden scaffold higher ALP activity and ECM mineral deposition through Runx2, OPN and OCN pathways. Conclusion Isotropic sustained release of ICA grant cell laden scaffolds rapid bone regeneration compacity which can provide a good osteogenic environment for loaded MSCs.

show abstract

“…As an example, Turk et al incorporated 10% graphene directly in the glass matrix before sintering borate-based porous scaffolds, doubling the compressive strength and obtaining an electrical conductivity (0.060 S/cm) which could be exploited to electrically stimulate cell growth [ 76 ]. Moreover, Deliormanlı et al fabricated more chemically stable and biocompatible silicate-based scaffolds coated with PCL/graphene with pore size between 100–500 μm, without detrimental effects of polymer coating on pore structure [ 77 ].…”

Section: Graphene-based Scaffoldsmentioning

confidence: 99%

Graphene-Based Scaffolds for Regenerative Medicine

et al. 2021

View full text Add to dashboard Cite

Leading-edge regenerative medicine can take advantage of improved knowledge of key roles played, both in stem cell fate determination and in cell growth/differentiation, by mechano-transduction and other physicochemical stimuli from the tissue environment. This prompted advanced nanomaterials research to provide tissue engineers with next-generation scaffolds consisting of smart nanocomposites and/or hydrogels with nanofillers, where balanced combinations of specific matrices and nanomaterials can mediate and finely tune such stimuli and cues. In this review, we focus on graphene-based nanomaterials as, in addition to modulating nanotopography, elastic modulus and viscoelastic features of the scaffold, they can also regulate its conductivity. This feature is crucial to the determination and differentiation of some cell lineages and is of special interest to neural regenerative medicine. Hereafter we depict relevant properties of such nanofillers, illustrate how problems related to their eventual cytotoxicity are solved via enhanced synthesis, purification and derivatization protocols, and finally provide examples of successful applications in regenerative medicine on a number of tissues.

show abstract

Investigation thein vitrobiological performance of graphene/bioactive glass scaffolds using MC3T3-E1 and ATDC5 cells

Cited by 7 publications

References 39 publications

Response of mouse bone marrow mesenchymal stem cells to graphene-containing grid-like bioactive glass scaffolds produced by robocasting

Response of mouse bone marrow mesenchymal stem cells to graphene-containing grid-like bioactive glass scaffolds produced by robocasting

Three-dimensional mesenchymal stem cell laden scaffold of icariin sustained-release for bone regeneration

Graphene-Based Scaffolds for Regenerative Medicine

Contact Info

Product

Resources

About