Investigations into the causes of mortality of the pearl oyster, Pinctada maxima (Jamson), in Western Australia

Pass, D. A.; Dybdahl, R.C.; Mannion, M.M.

doi:10.1016/0044-8486(87)90259-6

Cited by 108 publications

(64 citation statements)

References 12 publications

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“…Recently, Vibrio harveyi has appeared to be a primary pathogen infecting hatchery-reared fish and shellfish species over a wide geographical area (Pass et al, 1987;Pizzutto and Hirst, 1995;Vandenberghe et al, 1998;Zhang and Austin, 2000). Mortalities in hatchery-reared penaeid shrimps caused by Vibrio harveyi infection has resulted in severe economic losses in producing countries in Asia (Karunasagar et al, 1994;Lavilla-Pitogo et al, 1990;Liu et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

Conejero¹,

Hedreyda²

2004

J. Gen. Appl. Microbiol.

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The Vibrio harveyi hemolysin gene (vhh), which encodes for a virulence factor involved in pathogenicity to fish and shellfish species, may be targeted for species detection or strain differentiation. Primers designed for this gene were used in detection studies of V. harveyi strains from various hosts. One primer set among four tested, could amplify the expected gene fragment in PCR using templates from all 11 V. harveyi strains studied. Detection of the presence of the hemolysin gene could therefore serve as a suitable detection marker of Vibrio harveyi isolates potentially pathogenic to fish and shrimps.

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Section: Introductionmentioning

confidence: 99%

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

Conejero¹,

Hedreyda²

2004

J. Gen. Appl. Microbiol.

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“…V. harveyi has been associated with mortalities of penaeid shrimp larvae in the Philippines , Lavilla-Pitogo et al 1990, Thailand (Jiravanichpaisal et al 1994), India (Karunasagar et al 1994), Indonesia (Prayitno & Latchford 1995), Australia (Pizzutto & Hirst 1995), Venezuela (Alvarez et al 1998) and Ecuador (Robertson et al 1998). Additionally, there is an increasing list of aquatic animals for which V. harveyi has also been reported as a major pathogen, including finfish (Balebona et al 1995, Saeed 1995, bivalves (Pass et al 1987) and phyllosoma larvae of the lobster Jasus verreauxi (Diggles et al 2000). Karunasagar et al (1994) showed that antibioticresistant isolates of Vibrio harveyi could produce between 44 and 80% mortality in Penaeus monodon postlarvae after a 5 d exposure.…”

Section: Introductionmentioning

confidence: 99%

Virulence of luminous vibrios to Artemia franciscana nauplii

Soto-Rodríguez¹,

Roque²,

Lizárraga-Partida³

et al. 2003

Dis. Aquat. Org.

109

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From healthy and diseased penaeid shrimp from Asia and the Americas, 25 luminous and 2 non-luminous bacterial strains were isolated, and 14 were phenotypically identified as Vibrio harveyi; 9 isolates produced significant mortalities (45 to 80%) in Artemia franciscana nauplii at inoculation densities of 10 5 to 10 6 CFU ml -1 compared to the controls (unchallenged nauplii). The maximum number of bacteria ingested (bioencapsulated) by the Artemia nauplii varied from less than 10 to 10 3 CFU nauplius -1 and no significant relationship was observed between the density of bacteria inoculated, the amount of bacteria ingested, and naupliar mortality. Significant correlations were obtained between naupliar mortality and production of proteases, phospholipases or siderophores, but not between mortality and lipase production, gelatinase production, hydrophobicity or hemolytic activity. The results suggest that virulence of the strains tested was more related to the production of particular exoenzymes than to the measured colonization factors.

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“…In this study, several different pathogenic V. harveyi, Vibrio campbellii, and Vibrio parahaemolyticus isolates were shown to produce AI-2. Furthermore, disruption of AI-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic Artemia from the pathogenic isolates in in vivo challenge tests.Bacteria belonging to the species Vibrio harveyi and the closely related Vibrio campbellii and Vibrio parahaemolyticus are important pathogens in the intensive rearing of mollusks, finfish, and shrimp (1,7,10,11,14,19,25). The traditional control of bacterial disease in aquaculture relies on the use of antibiotics (3,21).…”

mentioning

confidence: 99%

Quorum Sensing-Disrupting Brominated Furanones Protect the Gnotobiotic Brine Shrimp Artemia franciscana from Pathogenic Vibrio harveyi , Vibrio campbellii , and Vibrio parahaemolyticus Isolates

Defoirdt

Crab

Wood

et al. 2006

Appl Environ Microbiol

167

133

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Autoinducer 2 (AI-2) quorum sensing was shown before to regulate the virulence of Vibrio harveyi towards the brine shrimp Artemia franciscana. In this study, several different pathogenic V. harveyi, Vibrio campbellii, and Vibrio parahaemolyticus isolates were shown to produce AI-2. Furthermore, disruption of AI-2 quorum sensing by a natural and a synthetic brominated furanone protected gnotobiotic Artemia from the pathogenic isolates in in vivo challenge tests.Bacteria belonging to the species Vibrio harveyi and the closely related Vibrio campbellii and Vibrio parahaemolyticus are important pathogens in the intensive rearing of mollusks, finfish, and shrimp (1,7,10,11,14,19,25). The traditional control of bacterial disease in aquaculture relies on the use of antibiotics (3,21). However, the frequent use of these compounds, in many cases even when pathogens are not evident, has led to the development and spread of resistance (3,10,13,23,24). Therefore, there is an urgent need for alternative control techniques. Disruption of quorum sensing, bacterial cell-to-cell communication by means of small signal molecules, has been suggested as a new anti-infective strategy for aquaculture (4).The quorum sensing system of V. harveyi has been shown to consist of three channels (8). Recently, we found that the autoinducer 2 (AI-2)-mediated channel of the system regulates the virulence of the bacterium towards the brine shrimp Artemia franciscana in vivo (5). Interestingly, halogenated furanones were found to interfere with AI-2 quorum sensing in Escherichia coli (16,17) and were shown before to block quorum sensing-regulated extracellular toxin production in V. harveyi, resulting in a reduced toxicity of cell-free culture fluids to Penaeus shrimp (12). Therefore, in this study, we aimed at investigating whether these halogenated furanones could reduce the virulence of V. harveyi and closely related bacteria in our model system with gnotobiotic Artemia franciscana.Detection of AI-2 production by the pathogenic isolates. In a first experiment, we aimed at detecting AI-2 production by the different pathogenic V. harveyi, V. campbellii, and V. parahaemolyticus isolates. The isolates tested are described in Table 1.Cell-free culture fluids of the isolates were prepared, and autoinducers were detected by a bioluminescent reporter assay as described before (22). The autoinducer receptor double mutant JMH597 (sensor HAI-1 Ϫ , sensor AI-2 ϩ , sensor CAI-1 Ϫ ) (8) was used as a reporter strain. The culture fluids of all isolates significantly induced bioluminescence in the reporter strain (data not shown), indicating that they all produced AI-2. The detection of AI-2 in cell-free culture supernatants of V. harveyi and V. parahaemolyticus confirms the report of Bassler et al. (2), who used the HAI-1 receptor mutant BB170 as a reporter strain. To the best of our knowledge, this is the first report mentioning AI-2 production by V. campbellii.Disruption of AI-2 quorum sensing in V. harveyi by the natural furanone. A second in vitro experim...

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Investigations into the causes of mortality of the pearl oyster, Pinctada maxima (Jamson), in Western Australia

Cited by 108 publications

References 12 publications

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

PCR detection of hemolysin (vhh) gene in Vibrio harveyi

Virulence of luminous vibrios to Artemia franciscana nauplii

Quorum Sensing-Disrupting Brominated Furanones Protect the Gnotobiotic Brine Shrimp Artemia franciscana from Pathogenic Vibrio harveyi , Vibrio campbellii , and Vibrio parahaemolyticus Isolates

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