Investigations of Vitamin D Receptor Polymorphism Affecting Affecting Workers Susceptibility to Lead.

Abstract

exposure status, smoking and alcohol drinking, were significantly associated with ICL and time-weighted ICL. Regression analyses suggested that exposure status and personal habits (smoking and alcohol drinking) are still the major effects on blood lead parameters for lead workers rather than genotypes. In conclusion, VDR genotypes did not significantly affect the long term blood lead parameters after adjustment for all factors, but industrial hygiene improvement, including engineering control and health education, are the most important ways to protect workers' health. (J Occup Health 2004; 46: 316-322)

A transdisciplinary perspective of chronic stress in relation to psychopathology throughout life span development

“…Genetic variance exists in an individual's ability to absorb, distribute, metabolize, and excrete ETs. For example, genotypes associated with heavy metal absorption and toxicity include hemochromatosis (Wright et al, 2004), vitamin D receptor (Chuang et al, 2004), and apolipoprotein-E (Godfrey, Wojcik, & Krone, 2003). ETs are principally industrial chemicals like lead (Pb), methylmercury (MeHg), and polychlorinated biphenyls (PCB) that can contribute to functional disruptions, neurodevelopmental delays, behavioral, as well as cognitive impairments throughout the life span (Bradley & Corwyn, 2002; Furumoto-Dawson, Gehlert, Sohmer, Olopade, & Sacks, 2007).…”

Does maternal VDR FokI single nucleotide polymorphism have an effect on lead levels of placenta, maternal and cord bloods?

“…Individual susceptibility due to genetic variations appears to be an important factor in lead toxicity in addition to dose, duration and timing of exposure [25]. The most related genes affecting lead toxicokinetics are delta-aminolevulinic acid dehydratase (ALAD) gene, Vitamin D receptor (VDR) gene and human hemochromatosis gene (HFE) [2,33,34].…”

Influence of VDR and HFE polymorphisms on blood lead levels of occupationally exposed workers

“…The restriction enzymes for VDR (rs1544410, rs7975232, and rs731236) and HFE (rs1799945 and rs1800562) were selected as previously described. 27 –29 Table S1 (Supplementary material) represents the dbSNP ID, primer sequence, restriction enzyme, annealing temperatures, and restriction pattern of the respective polymorphisms. The polymerase chain reaction performed in a 25 µL reaction volume contained genomic DNA, 4 mM dNTP, 10X PCR buffer, 25 mM MgCl 2 , forward, and reverse primers of 100 ng/µL each, and Taq polymerase under the following cycling parameters: initial denaturation at 95°C for 5 minutes, 35 cycles of 95°C for 30 seconds, annealing at 56°C for 30 seconds for VDR : rs1544410, VDR : rs7975232, VDR : rs731236 and HFE : rs1799945 SNPs/60°C for 30 seconds for HFE : rs1800562, elongation at 72°C for 30 seconds followed by a final extension of 72°C for 10 minutes.…”

“…The restriction enzymes for VDR (rs1544410, rs7975232, and rs731236) and HFE (rs1799945 and rs1800562) were selected as previously described. [27][28][29]…”