Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells

Bilkan, Mustafa Tuğfan; Çiçek, Zehra; Kurşun, Ayşe Gülnihal Canseven; Özler, Mehmet; Eşmekaya, Meriç Arda

doi:10.1007/s12032-022-01931-5

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Cancer cells often overexpress integrin receptors (αvβ3) on their cell surface [ 18 ]. Targeted delivery of TiO 2 NPs, which have shown cytotoxicity against melanoma cells, conjugated to a Arg-Gly-Asp (RGD) motif, would thus make PDT more selective, promoting binding with integrin αvβ3 [ 18 , 19 ]. TiO 2 NPs, conjugated to RGD, exhibit a cytotoxic effect in αvβ3 integrin-expressing mice melanoma cells but not in the normal cells lacking this integrin [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle-Based Treatment Approaches for Skin Cancer: A Systematic Review

et al. 2023

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Nanoparticles have shown marked promise as both antineoplastic agents and drug carriers. Despite strides made in immunomodulation, low success rates and toxicity remain limitations within the clinical oncology setting. In the present review, we assess advances in drug delivery nanoparticles, for systemic and topical use, in skin cancer treatment. A systematic review of controlled trials, meta-analyses, and Cochrane review articles was conducted. Eligibility criteria included: (1) a primary focus on nanoparticle utility for skin cancer; (2) available metrics on prevention and treatment outcomes; (3) detailed subject population; (4) English language; (5) archived as full-text journal articles. A total of 43 articles were selected for review. Qualitative analysis revealed that nanoscale systems demonstrate significant antineoplastic and anti-metastasis properties: increased drug bioavailability, reduced toxicity, enhanced permeability and retention effect, as well as tumor growth inhibition, among others. Nanoformulations for skin cancers have largely lagged behind those tested in other cancers–several of which have commercialized formulae. However, emerging evidence has indicated a powerful role for these carriers in targeting primary and metastatic skin cancers.

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Section: Resultsmentioning

confidence: 99%

Nanoparticle-Based Treatment Approaches for Skin Cancer: A Systematic Review

et al. 2023

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“…To evaluate whether the examined gD-derived peptides can interfere with the BTLA-HVEM interaction, we established an in vitro co-culture model of fully immunocompetent healthy T cells and melanoma cell line. SK-MEL-30 melanoma cell line is used as a model cell line in a wide range of cancer-related studies ( 56 , 57 ). Its high expression of both BTLA and HVEM was reported ( 58 ) and confirmed empirically (data not shown).…”

Section: Discussionmentioning

confidence: 99%

The effect of gD-derived peptides on T cell immune response mediated by BTLA-HVEM protein complex in melanoma patients

Wojciechowicz,

Kuncewicz,

Rutkowski

et al. 2024

Front. Immunol.

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IntroductionThe effector function of T cells is regulated via immune checkpoints, activating or inhibiting the immune response. The BTLA-HVEM complex, the inhibitory immune checkpoint, may act as one of the tumor immune escape mechanisms. Therefore, interfering with the binding of these proteins can prove beneficial in cancer treatment. Our study focused on peptides interacting with HVEM at the same place as BTLA, thus disrupting the BTLA-HVEM interaction. These peptides’ structure and amino acid sequences are based on the gD protein, the ligand of HVEM. Here, we investigated their immunomodulatory potential in melanoma patients.MethodsFlow cytometry analyses of activation, proliferation, and apoptosis of T cells from patients were performed. Additionally, we evaluated changes within the T cell memory compartment.ResultsThe most promising compound – Pep(2), increased the percentages of activated T cells and promoted their proliferation. Additionally, this peptide affected the proliferation rate and apoptosis of melanoma cell line in co-culture with T cells.DiscussionWe conclude that the examined peptide may act as a booster for the immune system. Moreover, the adjuvant and activating properties of the gD-derived peptide could be used in a combinatory therapy with currently used ICI-based treatment. Our studies also demonstrate that even slight differences in the amino acid sequence of peptides and any changes in the position of the disulfide bond can strongly affect the immunomodulatory properties of compounds.

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“…Cell viability was determined using the MTT assay method ( Bilkan et al, 2022 ). Briefly, MTT (5 mg/mL, 20 μL/per well) was added to the cells and incubated at 37°C for 4 h. Subsequently, the solutions were removed, DMSO (100 μL/well) was added to the wells, and absorbance was measured at 490 nm using a microplate reader (BioTek, USA).…”

Section: Methodsmentioning

confidence: 99%

Integrated network analysis and metabolomics reveal the molecular mechanism of Yinchen Sini decoction in CCl4-induced acute liver injury

Zheng,

Shi,

Meng

et al. 2023

Front. Pharmacol.

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Objective: Yinchen Sini decoction (YCSND), a traditional Chinese medicine (TCM) formula, plays a crucial role in the treatment of liver disease. However, the bioactive constituents and pharmacological mechanisms of action remain unclear. The present study aimed to reveal the molecular mechanism of YCSND in the treatment of acute liver injury (ALI) using integrated network analysis and metabolomics.Methods: Ultra-high-performance liquid chromatography coupled with Q-Exactive focus mass spectrum (UHPLC-QE-MS) was utilized to identify metabolites in YCSND, and high-performance liquid chromatography (HPLC) was applied to evaluate the quality of four botanical drugs in YCSND. Cell damage and ALI models in mice were established using CCl4. 1H-NMR metabolomics approach, along with histopathological observation using hematoxylin and eosin (H&E), biochemical measurements, and reverse transcription quantitative real-time PCR (RT-qPCR), was applied to evaluate the effect of YCSND on CCl4- induced ALI. Network analysis was conducted to predict the potential targets of YCSND in ALI.Result: Our results showed that 89 metabolites in YCSND were identified using UHPLC-QE-MS. YCSND protected against ALI by reducing the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) contents and increasing those of superoxide dismutase (SOD), and glutathione (GSH) both in vivo and in vitro. The 1H-NMRmetabolic pattern revealed that YCSND reversed CCl4-induced metabolic abnormalities in the liver. Additionally, the Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment analysis identified five pathways related to liver injury, including the PI3K-AKT, MAPK, HIF-1, apoptosis, and TNF signaling pathways. Moreover, RT-qPCR showed YCSND regulated the inflammatory response (Tlr4, Il6, Tnfα, Nfκb1, Ptgs2, and Mmp9) and apoptosis (Bcl2, Caspase3, Bax, and Mapk3), and inhibited PI3K-AKT signaling pathway (Pi3k and Akt1). Combined network analysis and metabolomics showed a link between the key targets (Tlr4, Ptgs2, and Mmp9) and vital metabolites (choline, xanthine, lactate, and 3-hydroxybutyric acid) of YCSND in ALI.Conclusion: Overall, the results contribute to the understanding of the therapeutic effects of YCSND on ALI, and indicate that the integrated network analysis and metabolomics could be a powerful strategy to reveal the pharmacological effects of TCM.

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Investigations on effects of titanium dioxide (TiO2) nanoparticle in combination with UV radiation on breast and skin cancer cells

Cited by 4 publications

References 25 publications

Nanoparticle-Based Treatment Approaches for Skin Cancer: A Systematic Review

Nanoparticle-Based Treatment Approaches for Skin Cancer: A Systematic Review

The effect of gD-derived peptides on T cell immune response mediated by BTLA-HVEM protein complex in melanoma patients

Integrated network analysis and metabolomics reveal the molecular mechanism of Yinchen Sini decoction in CCl4-induced acute liver injury

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