Investigations on Effects of Titanium Dioxide (TiO2) Nanoparticle in Combination with UV Radiation on Breast and Skin Cancer Cells

Abstract

In this study, we have investigated the chemotherapeutic potential of titanium dioxide (TiO2) nanoparticles on skin and breast cancer cells. The cells have treated with a 75 µg/ml concentration of titanium dioxide and then exposed to UV-A radiation. The combined effects of titanium dioxide and UV-A radiation on cell viability, cell cycle, plasma membrane, mitochondrial membrane potentials and apoptotic activity of the cells were investigated. As a result of the research, an increase was observed in the viability of cells treated with 75 µg/ml titanium dioxide concentration, while a significant decrease in cell viability was observed for both cell types when UV-A radiation and TiO2 were applied together. The results also showed that the percentage of apoptotic cells increased as a result of UV + TiO2 exposure. Accordingly, it can be said that TiO2 nanoparticles may research as potential chemotherapeutic agents for skin and breast cancers, especially in the presence of UV radiation.

Integrated network analysis and metabolomics reveal the molecular mechanism of Yinchen Sini decoction in CCl4-induced acute liver injury

“…Cell viability was determined using the MTT assay method (Bilkan et al, 2022). Briefly, MTT (5 mg/mL, 20 μL/per well) was added to the cells and incubated at 37 °C for 4 h. Subsequently, the solutions were removed, DMSO (100 μL/well) was added to the wells, and absorbance was measured at 490 nm using a microplate reader (BioTek, USA).…”