Investigations on the interface of nucleic acid aptamers and binding targets

Cai, Shundong; Yan, Jun; Xiong, Hongjie; Liu, Yanfei; Peng, Dongming; Liu, Zhenbao

doi:10.1039/c8an01467a

Cited by 220 publications

(145 citation statements)

References 183 publications

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“…Aptamers are short single‐stranded oligonucleotides (10–20 kDa), such as DNA, RNA, or modified nucleic acid sequences, which exhibit high affinity and specificity for binding to specific biological targets due to their unique secondary and tertiary conformational structures . Aptamers that generally contain 20–100 nucleotides have been screened and synthesized to bind various targets, including receptors and antibodies . The advantages of using aptamers as targeting ligands includes their high sensitivity and specificity, low immunogenicity, simple and reproducible synthesis, the ability to obtain high affinity ligands against a variety of substrates, and their simplicity for chemical modification; for instance, adding –SH groups for bioconjugation or modification of the backbones for resistance to DNases or RNases.…”

Section: Targeting Moieties On Nanocarriersmentioning

confidence: 99%

“…[33] Aptamers that generally contain 20-100 nucleotides have been screened and synthesized to bind various targets, including receptors and antibodies. [34] The advantages of using aptamers as targeting ligands includes their high sensitivity and specificity, low immunogenicity, simple and reproducible synthesis, the ability to obtain high affinity ligands against a variety of substrates, and their simplicity for chemical modification; [35] for instance, adding -SH groups for bioconjugation or modification of the backbones for resistance to DNases or RNases. The first aptamer-installed nanocarrier was reported in 2004 for targeting the prostate-specific membrane antigen (PSMA) in tumors, [36] and more aptamer nanocarriers have appeared since then.…”

Section: Arsenal Of Targeting Moietiesmentioning

confidence: 99%

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Ligand‐Installed Nanocarriers toward Precision Therapy

2019

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Development of drug‐delivery systems that selectively target neoplastic cells has been a major goal of nanomedicine. One major strategy for achieving this milestone is to install ligands on the surface of nanocarriers to enhance delivery to target tissues, as well as to enhance internalization of nanocarriers by target cells, which improves accuracy, efficacy, and ultimately enhances patient outcomes. Herein, recent advances regarding the development of ligand‐installed nanocarriers are introduced and the effect of their design on biological performance is discussed. Besides academic achievements, progress on ligand‐installed nanocarriers in clinical trials is presented, along with the challenges faced by these formulations. Lastly, the future perspectives of ligand‐installed nanocarriers are discussed, with particular emphasis on their potential for emerging precision therapies.

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Section: Targeting Moieties On Nanocarriersmentioning

confidence: 99%

Section: Arsenal Of Targeting Moietiesmentioning

confidence: 99%

Ligand‐Installed Nanocarriers toward Precision Therapy

2019

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“…Detection of cells using SPRs has some limitations that have been creatively overcome. The first limitation includes nonselective binding that causes the refractive index changes, which can be circumvented by reference flow cells to offset this effect [55]. Second is the sensing range, which is typically around 200 nm compared with cell dimensions that are in the micron range.…”

Section: Acoustic Aptasensorsmentioning

confidence: 99%

Aptamers for Diagnostics with Applications for Infectious Diseases

İlgü¹,

Fazlioglu²,

Ozturk³

et al. 2019

Recent Advances in Analytical Chemistry

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Aptamers are in vitro selected oligonucleotides (DNA, RNA, oligos with modified nucleotides) that can have high affinity and specificity for a broad range of potential targets with high affinity and specificity. Here we focus on their applications as biosensors in the diagnostic field, although they can also be used as therapeutic agents. A small number of peptide aptamers have also been identified. In analytical settings, aptamers have the potential to extend the limit of current techniques as they offer many advantages over antibodies and can be used for real-time biomarker detection, cancer clinical testing, and detection of infectious microorganisms and viruses. Once optimized and validated, aptasensor technologies are expected to be highly beneficial to clinicians by providing a larger range and more rapid output of diagnostic readings than current technologies and support personalized medicine and faster implementation of optimal treatments.

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“…These different conditions impact aptamer function by influencing intermolecular interactions as well as stabilities and structures of RNA aptamer itself. [6b,10] Thus, aptamer sequence must be fine‐tuned or the aptamers must be reselected in the environmental conditions, in which the aptamer will function.…”

Section: Numbers Of Analyzed Nucleobases At Randomized Position Of R‐mentioning

confidence: 99%

RNA‐Capturing Microsphere Particles (R‐CAMPs) for Optimization of Functional Aptamers

Endoh

Ohyama

Sugimoto

2019

Small

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RNA aptamers are useful building blocks for constructing functional nucleic acid‐based nanoarchitectures. The abilities of aptamers to recognize specific ligands have also been utilized for various biotechnological applications. Solution conditions, which can differ depending on the application, impact the affinity of the aptamers, and thus it is important to optimize the aptamers for the solution conditions to be employed. To simplify the aptamer optimization process, an efficient method that enables re‐selection of an aptamer from a partially randomized library is developed. The process relies on RNA‐capturing microsphere particles (R‐CAMPs): each particle displays different clones of identical DNA and RNA sequences. Using a fluorescence‐activated cell sorter, the R‐CAMPs that are linked to functional aptamers are sorted. It is demonstrated that after a single round of reselection, several functional aptamers, including the wild‐type, are selected from a library of 16 384 sequences. The selection using R‐CAMPs is further performed under the solution containing high concentration of ethylene glycol, suggesting applicability in various conditions to optimize an aptamer for a particular application. As any type of RNA clone can be displayed on the microspheres, the technology demonstrated here will be useful for the selection of RNAs based on diverse functions.

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Investigations on the interface of nucleic acid aptamers and binding targets

Abstract: The binding targets of aptamers, the interaction probing methods, and the main binding forces mediating the interaction have been summarized.

Cited by 220 publications

References 183 publications

Ligand‐Installed Nanocarriers toward Precision Therapy

Ligand‐Installed Nanocarriers toward Precision Therapy

Aptamers for Diagnostics with Applications for Infectious Diseases

RNA‐Capturing Microsphere Particles (R‐CAMPs) for Optimization of Functional Aptamers

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