Investigations on the Preservation of Blood by Rapid Freezing and Thawing Procedures

Rinfret, A. P.; Doebbler, G. F.; Cowley, C. W.

doi:10.1159/000426467

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…intracellular protective additives and slow freezing methods [7,8], 2. extracellular protective additives combined with rapid freezing and thawing procedures [13,15].…”

mentioning

confidence: 99%

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

View full text Add to dashboard Cite

“…intracellular protective additives and slow freezing methods [7,8], 2. extracellular protective additives combined with rapid freezing and thawing procedures [13,15].…”

mentioning

confidence: 99%

Glycerol Treated Human Red Cells Frozen with Liquid Nitrogen

Krijnen¹,

Wit²,

Kuivenhoven³

et al. 1964

Vox Sanguinis

View full text Add to dashboard Cite

“…Early studies on macromolecular cryoprotectants go back to Rinfret and coworkers [12] who utilized PVP successfully for freezing erythrocytes. In 1967 Knorpp and co-workers [13] described the successful cryopreservation of human RBC using HES and liquid nitrogen (LN2).…”

Section: Cryopreservation With Hydroxyethyl Starchmentioning

confidence: 99%

Cryopreservation of Erythrocytes, Thrombocytes, and Lymphocytes

Sputtek

Kühnl

Rowe

2007

Transfus Med Hemother

View full text Add to dashboard Cite

The cryopreservation of blood cells can be regarded as a classical field of development and application of low temperature biology. Cryopreservation methods have been developed for erythrocytes, which are commonly frozen with glycerol as the cryoprotective additive although hydroxyethyl starch (HES) shows considerable promise. Cryopreserved erythrocytes for transfusion are of advantage in the case of patients with rare blood groups, adverse antibody problems, autologous use and civil as well as military disasters. Additionally they can be used for blood typing, antibody screening and compatibility testing. Cryopreservation methods for thrombocytes, lymphocytes and hematopoietic stem cells usually involve dimethyl sulfoxide (DMSO) as the cryoprotective additive. Low temperature preservation of thrombocytes offers the possibility of making HPA- and/or HLA-typed platelet concentrates available in blood banks at any time. The use of cryopreserved lymphocytes is well established and a routine procedure for clinical laboratory testing. Recently there is a growing clinical interest in cryopreserved lymphocytes in addition to hematopoietic progenitor cells for the supplemental treatment of patients after blood stem cell transplantation. Despite occasional reports, it is our opinion that no clinically suitable method for the preservation of human granulocytes has been developed so far.

show abstract

“…In the recently described techniques [12] of preserving red cells by rapid freezing to temperatures below -100° C followed by rapid thawing, a variable fraction of the red cells is destroyed; some cells are haemolysed during the procedure, and an additional fraction does not survive the immediate post-transfusion period [9].…”

mentioning

confidence: 99%