Involvement of abscisic acid and hydrogen peroxide in regulating the activities of antioxidant enzymes in leaves of rice seedlings under magnesium deficiency

Chao, Yun-Yang; Chou, Ting-Shao; Kao, Ching Huei

doi:10.1007/s10725-011-9623-9

Cited by 16 publications

(11 citation statements)

References 37 publications

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“…Tan et al [84] studying five genes of the NCED family in Arabidopsis reported that over a period of 35 h there was increased NCED5 expression in flowers and leaves under water stress conditions. Chao et al [86] found that Mg deficiency resulted in an increase in ABA concentrations in leaves of Oryza sativa . This was also found in the current study with the increased expression of NCED5 in sensitive genotype C. SUL-4 ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular, Physiological and Biochemical Responses of Theobroma cacao L. Genotypes to Soil Water Deficit

et al. 2014

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Six months-old seminal plants of 36 cacao genotypes grown under greenhouse conditions were subjected to two soil water regimes (control and drought) to assess, the effects of water deficit on growth, chemical composition and oxidative stress. In the control, soil moisture was maintained near field capacity with leaf water potentials (ΨWL) ranging from −0.1 to −0.5 MPa. In the drought treatment, the soil moisture was reduced gradually by withholding additional water until ΨWL reached values of between −2.0 to −2.5 MPa. The tolerant genotypes PS-1319, MO-20 and MA-15 recorded significant increases in guaiacol peroxidase activity reflecting a more efficient antioxidant metabolism. In relation to drought tolerance, the most important variables in the distinguishing contrasting groups were: total leaf area per plant; leaf, stem and total dry biomass; relative growth rate; plant shoot biomass and leaf content of N, Ca, and Mg. From the results of these analyses, six genotypes were selected with contrasting characteristics for tolerance to soil water deficit [CC-40, C. SUL-4 and SIC-2 (non-tolerant) and MA-15, MO-20, and PA-13 (tolerant)] for further assessment of the expression of genes NCED5, PP2C, psbA and psbO to water deficit. Increased expression of NCED5, PP2C, psbA and psbO genes were found for non-tolerant genotypes, while in the majority of tolerant genotypes there was repression of these genes, with the exception of PA-13 that showed an increased expression of psbA. Mutivariate analysis showed that growth variables, leaf and total dry biomass, relative growth rate as well as Mg content of the leaves were the most important factor in the classification of the genotypes as tolerant, moderately tolerant and sensitive to water deficit. Therefore these variables are reliable plant traits in the selection of plants tolerant to drought.

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Section: Discussionmentioning

confidence: 99%

Molecular, Physiological and Biochemical Responses of Theobroma cacao L. Genotypes to Soil Water Deficit

et al. 2014

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“…The homogenate was centrifuged at 12,000 g for 20 min and the resulted supernatant was used for determination of enzyme activity. For CAT activity was performed as described previously (Chao et al 2012 ; Kato and Shimizu 1985 ). The decrease in H 2 O 2 was determined as the decrease in absorbance at 240 nm with a spectrophotometer (Metertec SP8001).…”

Section: Methodsmentioning

confidence: 99%

Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence

Yang

2016

Rice

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BackgroundFlooding due to global climate change is a serious problem that frequently decreases crop yields. Rice fields in flood-prone areas often experience full or partial submergence. Submergence has an adverse effect on internal oxygen availability, sugar status and survival. Complete submergence imposes severe pressure on plants, principally because the excess water in their surroundings deprives them of certain basic resources such as oxygen, carbon dioxide and light for photosynthesis. To better understand the mechanisms involved under different levels of flooding, it is necessary to further observe physiological responses and to identify the Rboh genes involved and determine how they are regulated during submergence.ResultsIn this study, significant physiological changes were observed in plant height, leaf sheath elongation and chlorophyll a, b and total content under partial and full submergence treatments. Senescence-regulating genes were severely affected under full submergence. Additionally, intracellular oxidative homeostasis was disrupted by overproduction of H2O2 and O2−, which affected cell viability and antioxidant enzyme activity, under different levels of submergence. Quantitative RT-PCR analyses revealed that complex regulation of Rboh genes is involved under different levels of submergence.ConclusionOur results demonstrated that the effect of physiological and the transcript levels of OsRboh genes were presented different responses to different levels of submergence in rice seedlings. There have different mechanism in intracellular to response different levels of submergence. Finally we discuss effects of the regulation of OsRboh expression and ROS production which was important to maintain homeostasis to help rice seedlings face different levels of submergence.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0074-9) contains supplementary material, which is available to authorized users.

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“…In addition, Mg also act as a cofactor and allosteric modulator for more than 300 enzymes including ribulose-1,5-bisphosphate carboxylase (Rubisco), ATPase, protein kinases, RNA polymerase, phosphatases and glutathione synthase [ 1 , 4 ]. Therefore, Mg also functions in many other physiological processes, such as respiration [ 7 ], glycolysis, tricarboxylic acid (TCA) cycle [ 8 ], energy transfer via adenosine triphosphate [ 9 ], carbohydrate partitioning between source and sink organs [ 1 , 10 ], reactive oxygen species (ROS) formation and related photooxidative damage [ 5 , 11 ], protein biosynthesis, and the formation of DNA and RNA [ 3 ]. Accordingly, a number of studies have investigated the effects of Mg-deficiency on Chl synthesis, transport and utilization of photosynthates [ 1 ], photochemical reactions, CO 2 fixation [ 2 , 4 - 6 ], respiration, TCA cycle [ 7 , 8 ], and ROS metabolism [ 4 , 5 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

Peng

Lee

et al. 2015

BMC Genomics

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BackgroundMagnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM MgSO4) or Mg-sufficient (1 mM MgSO4) nutrient solution for 16 weeks. Thereafter, we first investigated the proteomic responses of C. sinensis roots and leaves to Mg-deficiency using two-dimensional electrophoresis (2-DE) in order to (a) enrich our understanding of the molecular mechanisms of plants to deal with Mg-deficiency and (b) understand the molecular mechanisms by which Mg-deficiency lead to a decrease in photosynthesis.ResultsFifty-nine upregulated and 31 downregulated protein spots were isolated in Mg-deficient leaves, while only 19 upregulated and 12 downregulated protein spots in Mg-deficient roots. Many Mg-deficiency-responsive proteins were involved in carbohydrate and energy metabolism, followed by protein metabolism, stress responses, nucleic acid metabolism, cell wall and cytoskeleton metabolism, lipid metabolism and cell transport. The larger changes in leaf proteome versus root one in response to Mg-deficiency was further supported by our observation that total soluble protein concentration was decreased by Mg-deficiency in leaves, but unaffected in roots. Mg-deficiency had decreased levels of proteins [i.e. ribulose-1,5-bisphosphate carboxylase (Rubisco), rubisco activase, oxygen evolving enhancer protein 1, photosynthetic electron transfer-like protein, ferredoxin-NADP reductase (FNR), aldolase] involved in photosynthesis, thus decreasing leaf photosynthesis. To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism.ConclusionsOur results demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long-term Mg-deficiency. Mg-deficiency decreased the levels of many proteins involved in photosynthesis, thus decreasing leaf photosynthesis.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-1462-z) contains supplementary material, which is available to authorized users.

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Involvement of abscisic acid and hydrogen peroxide in regulating the activities of antioxidant enzymes in leaves of rice seedlings under magnesium deficiency

Cited by 16 publications

References 37 publications

Molecular, Physiological and Biochemical Responses of Theobroma cacao L. Genotypes to Soil Water Deficit

Molecular, Physiological and Biochemical Responses of Theobroma cacao L. Genotypes to Soil Water Deficit

Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence

Proteomic analysis of Citrus sinensis roots and leaves in response to long-term magnesium-deficiency

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