Involvement of abscisic acid-dependent and — Independent pathways in the upregulation of antioxidant enzyme activity during NaCl stress in cotton callus tissue

Bellaire, B; Carmody, J.; Braud, J. A. H.; Gossett, D. R.; Banks, Stephen W.; Lucas, Mariana; Fowler, Tim

doi:10.1080/10715760000301071

Cited by 76 publications

(49 citation statements)

References 51 publications

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“…Like CAT, APOX and GPX break down H 2 O 2 to H 2 O and O 2 . A comparable increase in APOX activity was induced by salt stress in Raphanus sativus plants (Lopez et al 1996) and in cotton callus tissue (Bellaire et al 2000). Overexpression of APOX enhanced the tolerance to salt stress and water deficit in tobacco chloroplast was observed (Badawi et al 2004).…”

Section: Resultsmentioning

confidence: 69%

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Anuradha¹,

Rao²

2007

Plant Soil Environ.

103

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The effect of 24-epibrassinolide and 28-homobrassinolide on seed germination and seedling growth of radish (Raphanus sativus L.) was studied under cadmium toxicity. The impact of brassinosteroids (BRs) on free proline levels and the activity of antioxidant enzymes catalase (CAT; EC 1.11.1.6), peroxidase (POD; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1), ascorbic peroxidase (APOX; EC 1.11.1.11) and guaiacol peroxidase (GPX; EC 1.11.1.7) in radish seedlings under Cd toxicity was evaluated. The effect of BRs on the activity of ascorbic acid oxidase (AAO; EC 1.10.3.3) and lipid peroxidation in radish seedlings challenged with Cd stress was also investigated. BRs supplementation alleviated the toxic effect of the heavy metal and increased the percentage of seed germination and seedling growth. Out of the two substances, HBL was found to be more effective than EBL in stress alleviation. HBL (3µM) alleviated the toxic effect of the heavy metal and increased the percentage of seed germination by 57% over Cd and 20% over unstressed control. Similarly supplementation of HBL (3µM) caused an increase of 156%, 78% and 91% in length, fresh weight and dry weight of seedling, respectively, over Cd treatment alone. The amelioration of seedling growth by BRs under metal toxicity was associated with enhanced levels of free proline. The activities of antioxidant enzymes CAT, SOD, APOX and GPX were increased in the seedlings from treatments with Cd along with BRs. Brassinosteroid treatment reduced the activity of POD and AAO in heavy metal stressed seedlings. Lipid peroxidation induced by Cd was found reduced with the supplementation of BRs. The results obtained in the study clearly indicated the ameliorative influence of brassinosteroids on the inhibitory effect of Cd toxicity.

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Section: Resultsmentioning

confidence: 69%

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Anuradha¹,

Rao²

2007

Plant Soil Environ.

103

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“…To cope with oxidative stress, plants have evolved several enzymatic and non-enzymatic systems. The channels by which H 2 O 2 mediates the induction of stomata closure, is by inducing the expression of antioxidant genes SOD (Sakamoto et al 1995;Guan and Scandalios 1998a;Bueno et al 1998), and CAT (Guan and Scandalios 1998b;Guan et al 2000), and increase the activities of antioxidative enzymes such as SOD, CAT, guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) in plant tissues (Prasad et al 1994;Gong et al 1998;Bellaire et al 2000). SOD reduces superoxide radicals (O 2 -) to H 2 O 2 , CAT reduces H 2 O 2 to water and dioxygen, APX reduces H 2 O 2 to water and monodehydroascorbate, thus preventing the formation of the highly reactive hydroxyl radical (OH) that can cause lipid peroxidation, protein denaturation, and DNA mutations.…”

Section: Discussionmentioning

confidence: 99%

“…Abscisic acid (ABA) regulates many important aspects of plant development, including the synthesis of seed storage proteins and lipids, the promotion of seed desiccation tolerance and dormancy, and the inhibition of the phase transitions from embryonic to germinative growth and from vegetative to reproductive growth (Bellaire et al 2000). ABA plays an important role in embryo development as well as growth and development throughout the plant life cycle.…”

Section: Introductionmentioning

confidence: 99%

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Zhang

Han

Yang

et al. 2009

Plant Cell Tiss Organ Cult

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Hydrogen peroxide (H 2 O 2 ) content and transcript levels of genes encoding superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) antioxidant enzymes were investigated during different stages of somatic embryogenesis in Larix leptolepis. H 2 O 2 content was lowest on day 0 when embryogenic callus was incubated on Murashige and Skoog (MS) medium supplemented with polyethylene glycol (PEG). This content began to increase when callus was transferred to the same medium but containing abscisic acid (ABA), and reached a peak at day 3 following incubation. The level of H 2 O 2 dropped from day 7 to day 10, peaked at day 21, then dropped again at day 24 and day 35, but increased when somatic embryos reached maturity at day 45. Transcript levels of SOD, CAT, and APX were lowest when somatic embryos were cultured on callus induction medium without ABA. When calli were transferred onto somatic embryo maturation medium, expression patterns of SOD, CAT, and APX varied, and transcript levels at all stages were higher than those at d 0. SOD expression was highest at day 3. Whereas, CAT expression levels were low during early stages of somatic embryogenesis, but increased at day 21, declined at days 24 and 35, and then began to increase again at day 45. APX gene expression patterns were highest at days 3, 21, and 45. These results suggested that ABA was essential for promoting somatic embryogenesis of L. leptolepis. Moreover, ABA induced production of H 2 O 2 and other active oxygen species (AOS), and mediated CAT, SOD, and APX gene expression in somatic embryogenesis of L. leptolepis.

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“…Abscisic acid (ABA), as a stress signal, enhances the tolerance of plants to several environmental stresses, including low temperature (Verslues and Zhu, 2005), salt (Bellaire et al, 2000), and drought (Ma et al, 2008). The increased tolerance is partly due to an enhanced antioxidant defense system that includes SOD, CAT, APX, and GR (Bellaire et al, 2000), and the AsA and GSH, which prevent the accumulation of ROS (Jiang and Zhang, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The increased tolerance is partly due to an enhanced antioxidant defense system that includes SOD, CAT, APX, and GR (Bellaire et al, 2000), and the AsA and GSH, which prevent the accumulation of ROS (Jiang and Zhang, 2002). Stylosanthes guianensis seedlings pretreated with ABA show increased chilling tolerance via increases in SOD activity and AsA and GSH levels, which provide little information about how AsA-GSH metabolism (e.g., APX, MDHAR, DHAR, and GR) regulates the scavenging of excess ROS (Zhou et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

Guo¹,

Chen²,

Gong³

et al. 2012

Genet. Mol. Res.

114

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ABSTRACT. To elucidate how physiological and biochemical mechanisms of chilling stress are regulated by abscisic acid (ABA) pretreatment, pepper variety (cv. 'P70') seedlings were pretreated with 0.57 mM ABA for 72 h and then subjected to chilling stress at 10°/6°C (day/night). Chilling stress caused severe necrotic lesions on the leaves and increased malondialdehyde and H 2 O 2 levels. Activities of monodehydroascorbate reductase (DHAR), dehydroascorbate reductase, glutathione reductase, guaiacol peroxidase, ascorbate peroxidase, ascorbate, and glutathione increased due to chilling stress during the 72 h, while superoxide dismutase and catalase activities decreased during 24 h, suggesting that chilling stress activates the AsA-GSH cycle under catalase deactivation in pepper leaves. ABA pretreatment induced significant increases in the above-mentioned enzyme activities and progressive decreases in ascorbate and glutathione levels. On the other hand, ABA-pretreated seedlings under chilling stress increased superoxide dismutase and guaiacol peroxidase activities and lowered concentrations of other antioxidants compared with untreated chilling-stressed plants. These seedlings showed concomitant decreases in foliage damage symptoms, and levels of malondialdehyde and H 2 O 2 . Induction of Mn-SOD and POD was observed in chilling-stressed plants treated with ABA. The expression of DHAR1 and DHAR2 was altered by chilling stress, but it was higher in the presence than in the absence of ABA at 24 h. Overall, the results indicate that exogenous application of ABA increases tolerance of plants to chilling-induced oxidative damage, mainly by enhancing superoxide dismutase and guaiacol peroxidase activities and related gene expression.

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Involvement of abscisic acid-dependent and — Independent pathways in the upregulation of antioxidant enzyme activity during NaCl stress in cotton callus tissue

Cited by 76 publications

References 51 publications

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

The effect of brassinosteroids on radish (Raphanus sativus L.) seedlings growing under cadmium stress

Changes in H2O2 content and antioxidant enzyme gene expression during the somatic embryogenesis of Larix leptolepis

Exogenous abscisic acid increases antioxidant enzymes and related gene expression in pepper (Capsicum annuum) leaves subjected to chilling stress

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