Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures

Ben-Amar, Anis; Cobanov, P.; Ghorbel, Abdelwahed; Mliki, Ahmed; Reustle, Götz M.

doi:10.1007/s10535-010-0055-6

Cited by 16 publications

(4 citation statements)

References 28 publications

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“…Although the precise role of AGPs is unclear (Seguí-Simarro et al 2011), it seems that these compounds play an important function during embryogenesis in suspension cultures, e.g. in Daucus carrota (L.) (Marcel et al 1997; Oxley and Bacic 1999), Beta vulgaris (L) (Capataz-Tafur et al 2011), cotton (Poon et al 2012) and Cucurbita pepo pepo (Amar et al 2010) as well as in isolated microspore cultures of maize (Borderies et al 2004), wheat (Letarte et al 2006), Brassica napus (Tang et al 2006) and white cabbage (Yuan et al 2012). …”

Section: Discussionmentioning

confidence: 99%

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

et al. 2013

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Miscanthus × giganteus is a popular energy crop, which due to its hybrid origin is only vegetatively reproduced. Asexual embryogenesis in anther and microspore culture leading to double haploids production could allow to regain the ability for sexual reproduction and to increase the biodiversity of the species. Therefore, the goal of this paper was to investigate the requirements of androgenesis in Miscanthus. The standard protocols used for monocotyledonous plants were applied with many modifications regarding the developmental stage of the explants at the time of culture initiation, stress treatment applied to panicles and isolated anthers as well as various chemical and physical parameters of in vitro culture conditions. Our results indicated that the induction of androgenesis in M. × giganteus is possible. However, the very low efficiency of the process and the lack of regeneration ability of the androgenic structures presently prevent the use of this technique.

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Section: Discussionmentioning

confidence: 99%

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

et al. 2013

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“…The primary cell walls (PCW) are formed during cell division and early expansion, and are mainly composed of cellulose, hemicelluloses and pectins. The cellulose microfibrils are embedded in a hydrated matrix of polysaccharides which forms a three-dimensional network responsible for the physical, chemical and physiological properties of cell walls (Cosgrove 2005;Amar et al 2010;Albersheim et al 2010). The formation of the secondary cell wall occurs by lignin deposition, especially after cell expansion (Albersheim et al 1996;Cosgrove & Jarvis 2012;Fahey et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Early development of epiphytic roots: perspectives based on the composition of the velamen cell wall

et al. 2020

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The velamen, a root structure of some epiphytic species for water uptake, usually is stratified epidermis consisting of dead cells. In general, its cell walls exhibit variation during development, including in thickness and amount and type of impregnated substances. These changes result in diverse physical and chemical properties that can serve in water and nutrient uptake, as well as in mechanical support and protection. On this basis, the main objective of the current study was to describe the composition of the cell walls of the velamen during the development of the roots of four species of Cattleya. Anatomical, histochemical and immunocytochemical analyses were performed with samples (n=3 individuals per species) of meristematic, developing, and mature regions of the root. The development of the primary wall led to the deposition of pectins as highly methylesterified homogalacturonans, which were demetihylesterified with maturation of the velamen. The deposition of lipids (and subsequently lignins) in velamen cells marked a transition stage to the formation of the secondary wall, which gives rigidity to the tissue. For the first time, we showed that the deposition of lipids and lignins began close to the exodermis in the direction of the epivelamen.

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“…Although undefined, CMs obtained from higher plants are often used in biotechnological applications to support the proliferation of many different plant cells or tissues. In some species such media are essential for successful regeneration of fertile plants from microspores (Patel et al, 2004;Sidhu and Davies, 2009) and somatic hybrids (Horita et al, 2003), promotion of somatic embryogenesis (Ben Amar et al, 2010;Couillerot et al, 2012) or recovery of cryopreserved cells (Hargreaves et al, 2002). Preparing CM from higher plant cultures may be troublesome and laborious, so green algae culture may offer a better source of conditioned medium.…”

Section: Introductionmentioning

confidence: 99%

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

Zielińska¹,

Matusiak-Mikulin²,

Grabski³

et al. 2015

Acta Biologica Cracoviensia S. Botanica

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Conditioned medium (CM) is a general term describing media in which cells have already been cultivated for some time. Such media, usually clarified by filtration, have been used by plant biotechnologists as additives supporting the growth of cell suspensions, organs and whole plants. This study examined the effect of CM obtained from green alga Desmodesmus subspicatus on the growth and functioning of the photosynthetic apparatus of Nicotiana tabacum and Arabidopsis thaliana in culture in vitro. Plants where cultured on CM diluted 1.25-, 2-and 5-fold with MS medium. The increase in fresh and dry weight was highest in tobacco and Arabidopsis cultured on CM/2 and CM/1.25 media. Those two concentrations also increased the amount of chlorophylls in both plants tested. CM improved parameter PI (reflecting the photosynthetic "vitality" of the organism) and electron transport efficiency, and increased the fraction of active reaction centers. Analysis of chlorophyll fluorescence in vivo suggests that the improvement of these plants grown in the presence of algal CM may result from stimulation of photosynthesis. Algal CM offers a convenient, cheap, universal supplement for stimulating the growth of higher plants in vitro.K Ke ey y w wo or rd ds s: : Algal exudates, in vitro culture, growth improvement, photosynthesis.

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Involvement of arabinogalactan proteins in the control of cell proliferation of Cucurbita pepo suspension cultures

Cited by 16 publications

References 28 publications

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

Failure of androgenesis in Miscanthus × giganteus in vitro culture of cytologically unbalanced microspores

Early development of epiphytic roots: perspectives based on the composition of the velamen cell wall

Growth Improvement of Nicotiana and Arabidopsis In Vitro by Microalgal Conditioned Media

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