Involvement of Cathepsin B- and L-Like Proteinases in Silk Gland Histolysis during Metamorphosis of Bombyx mori

Shiba, Hiroshi; Uchida, Daisuke; Kobayashi, H.; Natori, Masahiko

doi:10.1006/abbi.2001.2343

Cited by 48 publications

(34 citation statements)

References 16 publications

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“…Programmed destruction of tissues that are no longer needed or that need to be renewed (i.e., during moulting or metamorphosis) is an essential developmental process in which cysteine proteases have been reported to be involved (32,39,46,60,66,78). In the flesh fly, S. peregrina, successful development relies on the production and secretion of family C1 cysteine proteases (32,38,39); an endogenous cystatin has been described, sarcocystatin A, which is believed to act as a modulator of cysteine protease activity during development, thereby protecting newly developing larval or adult tissues (55,64,65).…”

Section: Discussionmentioning

confidence: 99%

A Virus Essential for Insect Host-Parasite Interactions Encodes Cystatins

Espagne¹,

Douris

Lalmanach

et al. 2005

J Virol

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Section: Discussionmentioning

confidence: 99%

A Virus Essential for Insect Host-Parasite Interactions Encodes Cystatins

Espagne¹,

Douris

Lalmanach

et al. 2005

J Virol

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“…In insects, cathepsin B has been shown to be involved in several biological processes: digestion of food proteins in midgut (27)(28)(29); degradation and mobilization of yolk proteins during embryogenesis (30-34); and self-destruction in programmed cell death during metamorphosis (20,(35)(36)(37).…”

Section: Biological Function Of Cathepsin B In Soldiers Of T Styraci?mentioning

confidence: 99%

Venomous protease of aphid soldier for colony defense

Kutsukake

Shibao

Nikoh

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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In social aphids, morphological, behavioral, and physiological differences between soldiers and normal insects are attributed to differences in gene expression between them, because they are clonal offspring parthenogenetically produced by the same mothers. By using cDNA subtraction, we identified a soldierspecific cysteine protease of the family cathepsin B in a social aphid, Tuberaphis styraci, with a second-instar soldier caste. The cathepsin B gene was specifically expressed in soldiers and firstinstar nymphs destined to be soldiers. The cathepsin B protein was preferentially produced in soldiers and showed a protease activity typical of cathepsin B. The cathepsin B mRNA and protein were localized in the midgut of soldiers. For colony defense, soldiers attack enemies with their stylet, which causes paralysis and death of the victims. Notably, after soldiers attacked moth larvae, the cathepsin B protein was detected from the paralyzed larvae. Injection of purified recombinant cathepsin B protein certainly killed the recipient moth larvae. From these results, we concluded that the cathepsin B protein is a major component of the aphid venom produced by soldiers of T. styraci. Soldier-specific expression of the cathepsin B gene was found in other social aphids of the genus Tuberaphis. The soldier-specific cathepsin B gene showed an accelerated molecular evolution probably caused by the action of positive selection, which had been also known from venomous proteins of other animals.

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“…Additionally, CathB is observed in the granulocytes and plasmatocytes of H. armigera larvae specifically at the prepupation stage (sixth instar), implicating its role in larva to pupa transition (Yang et al, 2007). Also, in B. mori, CathB is expressed along with CathL for silk gland and fat body histolysis during pupation (Shiba et al, 2001;Wang et al, 2008). After pupation, the fat body and midgut of larvae must be Fig.…”

Section: Indispensability and Functionality Of Cathepsins In Insect Dmentioning

confidence: 98%