Involvement of Chemosensory Protein BodoCSP1 in Perception of Host Plant Volatiles in <i>Bradysia odoriphaga</i>

Chunni, Zhang; Tang, Bowen; Zhou, Taoling; Yu, Xiaoting; Hu, Manfei; Dai, Wu

doi:10.1021/acs.jafc.1c02807

Cited by 21 publications

(29 citation statements)

References 49 publications

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“…Our results show that the two hydrophobic amino acids valine and leucine appear most frequently in docking simulations. These amino acids are also key for the binding of other dipteran chemosensory proteins to ligands 47, 48 . Leucine and isoleucine might generally be related to ligand binding in insects and are also the key residues associated with binding in CSP MbraA6 and SfurCSP5 8 .…”

Section: Discussionmentioning

confidence: 99%

“…These amino acids are also key for the binding of other dipteran chemosensory proteins to ligands. 47,48 Leucine and isoleucine might generally be related to ligand binding in insects and are also the key residues associated with binding in CSP MbraA6 and SfurCSP5. 8 The specific binding of some ligands to CSP also mainly depends on shape recognition and hydrophobic interactions, such as Tyr98 and Asp67 in CSP4 of honeybees, and Leu30 in GmolCSP8 and Try11 in CSP2 of Bemisia tabaci.…”

Section: Discussionmentioning

confidence: 99%

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Identification and functional analysis of a chemosensory protein from Bactrocera minax (Diptera: Tephritidae)

Cui

Liu

Wang

et al. 2022

Pest Management Science

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Background: Olfaction has an indispensable role in insect behavior, enabling location of suitable host plants and oviposition sites, finding mates and evasion of natural enemies. Chemosensory proteins (CSPs) function to screen external odorants and transport them to olfactory receptor neurons, thereby increasing the sensitivity of the olfactory system. At present, CSP genes have been identified in many insect species, but there are relatively few studies on the function of CSP, especially in Tephritidae.Results: In this study, we sequenced and analyzed 12 transcriptomes of Bactrocera minax and identified five CSP genes. The results of polymerase chain reactions with reverse transcription showed that BminCSP3 was highly expressed only in antennae. Results from competitive binding experiments showed that BminCSP3 has good binding ability to citral compared with 23 other volatile organic compounds. The docking model with citral showed hydrogen bond formation with residues (ARG97); however, no hydrogen bonds were formed in the docking of five other ligands (furfuryl alcohol, linalool, cis-3-hexenyl acetate, (R)-(+)-limonene and (+)-carvone). Electroantennogram (EAG) analyses revealed that citral was active in B. minax at the antennal level, and the EAG response value of female adults was significantly higher than that of male adults. Furthermore, the results of behavioral bioassays showed that females were significantly attracted to citral. Conclusion: Our results suggest that BminCSP3 plays an important role in the recognition of citral by B. minax adults.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of a chemosensory protein from Bactrocera minax (Diptera: Tephritidae)

Cui

Liu

Wang

et al. 2022

Pest Management Science

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“…The results indicated that silencing of the BodoCSP4 gene significantly decreased the behavioral response of B. odoriphaga male adults to diallyl disulfide and n-heptadecane but did not decrease the behavioral response to methyl allyl disulfide. In contrast, Zhang et al (2021) reported that silencing of BodoCSP1 reduced the behavioral response of B. odoriphaga male adults to diallyl disulfide and methyl allyl disulfide. In addition, previous reports indicated that BodoOBP1 , BodoOBP2 ( Tang et al, 2019 ), BodoOBP5 ( Yang et al, 2021a ), and BodoOBP8 ( Yang et al, 2021b ) bind well to diallyl disulfide and methyl allyl disulfide.…”

Section: Discussionmentioning

confidence: 92%

“…The primer pairs dsCSP4 and dsGFP , containing the T7 promoter sequence, are listed in Table 1 , and the T7 RiboMAX ™ Express RNAi System was used to synthesize double stranded RNAs (dsRNAs). The dsRNAs were microinjected into 3-day-old pupae of B. odoriphaga , and newly eclosed male adults were selected for dsRNA extraction and behavioral assays as previously described ( Yang et al, 2021b ; Zhang et al, 2021 ). According to the fluorescence binding results, after BodoCSP4 was silenced, a Y-tube olfactometer was used to assess the behavioral responses of B. odoriphaga male adults to the ligands diallyl disulfide, methyl allyl disulfide, and n-heptadecane as described in our previous report ( Yang et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

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Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Yang

Hua²,

Zhu³

et al. 2022

Front. Physiol.

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Bradysia odoriphaga (Diptera: Sciaridae) is a serious pest of Chinese chives cultivated in China. Chemosensory proteins (CSPs) are important components of insect olfactory systems that capture and bind environmental semiochemicals which are then transported to olfactory receptors. Despite their importance, the mechanism of olfaction and related behavioral processes in B. odoriphaga have not been characterized. Here, we found that BodoCSP4 has an important olfactory function. RT-qPCR indicated that BodoCSP4 expression was highest in the heads (antennae removed) of adult males, followed by the antennae of adult males. Competitive binding assays with 33 ligands indicated that BodoCSP4 binds well with methyl allyl disulfide, diallyl disulfide, and n-heptadecane; the corresponding dissolution constants (Ki) were as high as 5.71, 5.71, and 6.85 μM, respectively. 3D-structural and molecular docking indicated that BodoCSP4 has five α-helices and surrounds the ligand with certain hydrophobic residues including Leu60, Leu63, Leu64, Ala67, Val28, Ile30, Ile33, Leu34, and Val86, suggesting these residues help BodoCSP4 bind to ligands. Silencing of BodoCSP4 significantly decreased the attraction of B. odoriphaga males to diallyl disulfide and n-heptadecane but not to methyl allyl disulfide in Y-tube olfaction assays. These results increase our understanding of how BodoCSP4 contributes to host and female localization by B. odoriphaga males.

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Candidate odorant‐binding protein and chemosensory protein genes in the turnip aphidLipaphis erysimi

Kuang,

Shangguan,

Yuan

et al. 2023

Arch Insect Biochem Physiol

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The turnip aphid, Lipaphis erysimi Kaltenbach, inflicts heavy damage on cruciferous crops worldwide. In these insects, olfactory perception is crucial for mating, host location, and oviposition. Both odorant‐binding proteins (OBPs) and chemosensory proteins (CSPs) are responsible for the delivery of host odorants and pheromones during initial molecular interactions. In this study, antennal and body transcriptomes of L. erysimi were generated through the deep sequencing of RNA libraries. A dataset of 11 LeryOBP and four LeryCSP transcripts was identified among assembled unigenes and subjected to sequence analysis. Phylogenetic analysis found a one‐to‐one orthologous relationship between LeryOBP/LeryCSP and its corresponding homologs from other aphid species. Further quantitative real‐time PCR analyses across developmental stages and tissues showed that five LeryOBP genes (i.e., LeryGOBP, LeryOBP6, LeryOBP7, LeryOBP9, and LeryOBP13) and LeryCSP10 were specifically or significantly elevated in the antennae compared with other tissues. Moreover, two transcripts (i.e., LeryGOBP and LeryOBP6) exhibited remarkably higher expression levels in alate aphids, implying their potentially functional role in the perception of new host plant locations. These results present the identification and expression of OBP/CSP genes in L. erysimi, providing valuable insights into their putative role in olfactory signal transduction.

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Involvement of Chemosensory Protein BodoCSP1 in Perception of Host Plant Volatiles in Bradysia odoriphaga

Cited by 21 publications

References 49 publications

Identification and functional analysis of a chemosensory protein from Bactrocera minax (Diptera: Tephritidae)

Identification and functional analysis of a chemosensory protein from Bactrocera minax (Diptera: Tephritidae)

Chemosensory protein 4 is required for Bradysia odoriphaga to be olfactory attracted to sulfur compounds released from Chinese chives

Candidate odorant‐binding protein and chemosensory protein genes in the turnip aphidLipaphis erysimi

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