Involvement of clathrin and -arrestins in Aggregatibacter actinomycetemcomitans endocytosis of human vascular endothelial cells

Wang, Qin; Li, Jie; JingYi, Yu; Zhong, Dan; Jia, Ning; Cao, Hong; Zhang, Jincai

doi:10.5897/ajmr2013.6334

Afr. J. Microbiol. Res.

2014

DOI: 10.5897/ajmr2013.6334

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Involvement of clathrin and -arrestins in Aggregatibacter actinomycetemcomitans endocytosis of human vascular endothelial cells

Qin Wang¹,

Jie Li²,

Yu JingYi³

et al.

Abstract: Phosphorylcholine (PC) has been reported to help PC-bearing Aggregatibacter actinomycetemcomitans to gain access to the general circulation via interaction with the platelet-activating factor receptor (PAFR) on endothelial cells. However, the underlying mechanism remains unclear. Here, we found that inhibition of clathrin and β-arrestins blocked bacterial PC binding to PAFR which significantly attenuated A. actinomycetemcomitans invasion of human vascular endothelial cells (HUVEC). These results demonstrated t… Show more

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Cited by 3 publications

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RETRACTED ARTICLE: Distinctive pathways characterize A. actinomycetemcomitans and P. gingivalis

Zhu

Liu

et al. 2014

Mol Biol Rep

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The study aimed to compare the molecular mechanism of Porphuromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans). With microarray dataset (GSE9723) from Gene Expression Omnibus, differentially expressed genes (DEGs) were identified comparing normal cell samples with A. actinomycetemcomitans-infected and P. gingivalis-infected periodontitis cell samples, respectively (|logFC| > 1, p value <0.01), followed by hierarchical cluster analysis using Cluster software. Network topological features of A. actinomycetemcomitans-related and P. gingivalis-related protein-protein interaction networks, and background network, which included average shortest path length (ASPL), degree, closeness centrality (CC), eccentricity (EC), betweenness centrality (BC) and topological coefficient (TC) were compared using network analysis plugin of Cytoscape, followed by pathway enrichment analysis (p value <0.05) using FISHER hyper-geometric algorithm and calculation of pathway alter scores using LIMMA. Totally, 839 DEGs and 251 DEGs were screened for A. actinomycetemcomitans and P. gingivalis, respectively. A. actinomycetemcomitans-related network had lower ASPL, degree and EC but higher CC and TC (p < 0.01), while P. gingivalis-related network had lower EC but higher CC and BC (p < 0.01) compared to background network. P. gingivalis-related network had lower ASPL, degree and EC, but higher CC than the A. actinomycetemcomitans-related network (p < 0.05). A. actinomycetemcomitans was associated with the pathways relating to endothelial cells function, while neuroactive ligand-receptor interaction and MAPK pathways were important for P. gingivalis, which had higher alter scores in hematopoietic cell lineage, hypertrophic cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy pathways than A. actinomycetemcomitans. Genes and pathways of the two pathogens were distinctive. The findings aided in preventing and treating relevant diseases.

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RETRACTED ARTICLE: Distinctive pathways characterize A. actinomycetemcomitans and P. gingivalis

Zhu

Liu

et al. 2014

Mol Biol Rep

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Isolation and genetic characterization of Nocardia seriolae from snubnose pompano Trachinotus blochii in Vietnam

Vu-Khac¹,

Duong²,

Chen³

et al. 2016

Dis. Aquat. Org.

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A total of 480 cage-cultured fish were collected from 4 coastal provinces in central Vietnam to investigate the causative agent of nocardiosis. Fish displayed unique characteristics such as paleness and lethargy and exhibited haemorrhages and ulcers on the skin. Prominent white nodules varying in size were observed in the spleen, kidney, and liver. Furthermore, histopathological sections showed typical granulomatous lesions in these organs. Using the Ziehl-Neelsen staining method, isolated bacteria exhibited acid-fast, bead-like filament morphology when cultured in brain-heart infusion medium or Ogawa medium. Phylogenetic analysis of 16S rDNA confirmed that the isolated bacterium was Nocardia seriolae. This study demonstrates for the first time an outbreak of N. seriolae in snubnose pompano in central Vietnam.

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Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China

et al. 2020

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Nocardia seriolae is the causative agent of nocardiosis in both marine and freshwater fish. Here, we report on multiple outbreaks of nocardiosis associated with elevated mortality (23-35%) in farmed largemouth bass in Sichuan, China, from 2017 to 2018. A total of 9 strains isolated from diseased largemouth bass were identified as N. seriolae by phenotypic characterization, 16S rRNA and hsp65 gene sequence analysis. The clinical signs of infected largemouth bass included hemorrhage, skin ulcers and prominent tubercles varying in size in the gill, liver, spleen and kidney. Experimental infection indicated that these isolates were the pathogens responsible for the mortalities. In vitro antibacterial activities of 12 antibiotics against N. seriolae isolates were determined as minimum inhibitory concentrations. Histopathological observation of diseased fish infected with N. seriolae showed necrotizing granulomatous hepatitis, nephritis, splenitis, epithelial hypertrophy and hyperplasia with degenerative changes of the epithelium in the gill. Large quantities of bacterial aggregates were found in the necrotic area of the granuloma by Lillie-Twort Gram stain and immunocytochemistry. Our findings indicated that N. seriolae is a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China.

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Involvement of clathrin and -arrestins in Aggregatibacter actinomycetemcomitans endocytosis of human vascular endothelial cells

Cited by 3 publications

References 22 publications

RETRACTED ARTICLE: Distinctive pathways characterize A. actinomycetemcomitans and P. gingivalis

RETRACTED ARTICLE: Distinctive pathways characterize A. actinomycetemcomitans and P. gingivalis

Isolation and genetic characterization of Nocardia seriolae from snubnose pompano Trachinotus blochii in Vietnam

Nocardia seriolae: a serious threat to the largemouth bass Micropterus salmoides industry in Southwest China

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