Involvement of Coenzyme M during Aerobic Biodegradation of Vinyl Chloride and Ethene by
            <i>Pseudomonas putida</i>
            Strain AJ and
            <i>Ochrobactrum</i>
            sp. Strain TD

Danko, Anthony S.; Saski, Christopher; Tomkins, Jeffrey; Freedman, David L.

doi:10.1128/aem.72.5.3756-3758.2006

Cited by 34 publications

(41 citation statements)

References 19 publications

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“…The same PCR conditions as in the original works were used (Hendrickson et al, 2002;Coleman and Spain, 2003a). The DNA samples were also subjected to PCR with specific primers for the EaCoMT gene (CoM-F1L and CoM-R2E) which codes for the enzyme epoxyalkane:coenzyme M transferase that is involved in the aerobic degradation of VC and ethene (Coleman and Spain, 2003b;Danko et al, 2006). DNA from Nocardioides sp JS 614 (Coleman et al, 2002) and from the KB-1 mixed culture (SiREM, Guelph, ON, Canada) was used as positive control for the EaCoMT gene and Dehalococcoides, respectively.…”

Section: Molecular Biology Methodsmentioning

confidence: 99%

“…The same DNA samples were also tested for the presence of the EaCoMT gene (Table 2) which is encoding a key enzyme in the aerobic oxidation of VC and ethene (Danko et al, 2006). The EaCoMT gene was detected at all sampling locations including the upgradient sampling location where a previous exposure to the contaminants is not reported.…”

Section: Microbial Analyses Of the Sedimentmentioning

confidence: 99%

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Evaluating the fate of chlorinated ethenes in streambed sediments by combining stable isotope, geochemical and microbial methods

Abe

Aravena

Zopfi

et al. 2009

Journal of Contaminant Hydrology

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a b s t r a c tThe occurrence of chlorinated ethene transformation in a streambed was investigated using concentration and carbon isotope data from water samples taken at different locations and depths within a 15 × 25 m study area across which a tetrachloroethene (PCE) plume discharges. Furthermore, it was evaluated how the degree of transformation is related to groundwater discharge rates, redox conditions, solid organic matter content (SOM) and microbial factors. Groundwater discharge rates were quantified based on streambed temperatures, and redox conditions using concentrations of dissolved redox-sensitive species. The degree of chlorinated ethene transformation was highly variable in space from no transformation to transformation beyond ethene. Complete reductive dechlorination to ethane and ethene occurred at locations with at least sulfate-reducing conditions and with a residence time in the samples streambed zone (80 cm depth) of at least 10 days. Among these locations, Dehalococcoides was detected using a PCR method where SOM contents were N 2% w/w and where transformation proceeded beyond ethene. However, it was not detected at locations with low SOM, which may cause an insufficient H 2 supply to sustain a detectably dense Dehalococcoides population. Additionally, it is possible that other organisms are responsible for the biodegradation. A microcosm study with streambed sediments demonstrated the potential of VC oxidation throughout the site even at locations without a pre-exposure to VC, consistent with the detection of the epoxyalkane:coenzyme M transferase (EaCoMT) gene involved in the degradation of chlorinated ethenes via epoxidation. In contrast, no aerobic transformation of cDCE in microcosms over a period of 1.5 years was observed. In summary, the study demonstrated that carbon isotope analysis is a sensitive tool to identify the degree of chlorinated ethene transformation even in hydrologically and geochemically complex streambed systems. In addition, it was observed that the degree of transformation is related to redox conditions, which in turn depend on groundwater discharge rates.

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Section: Molecular Biology Methodsmentioning

confidence: 99%

Section: Microbial Analyses Of the Sedimentmentioning

confidence: 99%

Evaluating the fate of chlorinated ethenes in streambed sediments by combining stable isotope, geochemical and microbial methods

Abe

Aravena

Zopfi

et al. 2009

Journal of Contaminant Hydrology

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“…Coenzyme M is an essential terminal methyl carrier in methanogenesis (Graham & White, 2002;Thauer, 1998) and promotes growth in some methanogenic members of the Archaea (Miller et al, 1986;Sprenger et al, 2000;. Coenzyme M has a role not only in the methanogenesis pathway in the Euryarchaeota, but also in the utilization of short-chain alkenes, as demonstrated in recent studies on propylene-, ethyleneand vinyl chloride-utilizing bacteria (Allen et al, 1999;Boyd et al, 2006;Coleman & Spain, 2003a, b;Danko et al, 2006;Mattes et al, 2005). Emission of trace amounts of methane is generally observed in the growing cultures of almost all Archaeoglobus species, but they are unable to grow by methanogenesis.…”

Section: K Mori and Others 814mentioning

confidence: 95%

Archaeoglobus infectus sp. nov., a novel thermophilic, chemolithoheterotrophic archaeon isolated from a deep-sea rock collected at Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean

Mori

Maruyama

Urabe

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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A novel thermophilic, strictly anaerobic archaeon, designated strain Arc51 T , was isolated from a rock sample collected from a deep-sea hydrothermal field in Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean. Cells of the isolate were irregular cocci with single flagella and exhibited blue-green fluorescence at 436 nm. The optimum temperature, pH and NaCl concentration for growth were 70 6C, pH 6.5 and 3 % (w/v), respectively. Strain Arc51 T could grow on thiosulfate or sulfite as an electron acceptor in the presence of hydrogen. This strain required acetate as a carbon source for its growth, suggesting that the reductive acetyl CoA pathway for CO 2 fixation was incomplete. In addition, coenzyme M (2-mercaptoethanesulfonic acid), which is a known methyl carrier in methanogenesis, was also a requirement for growth of the strain. Analysis of the 16S rRNA gene sequence revealed that the isolate was similar to members of the genus Archaeoglobus, with sequence similarities of 93.6-97.2 %; the closest relative was Archaeoglobus veneficus. Phylogenetic analyses of the dsrAB and apsA genes, encoding the alpha and beta subunits of dissimilatory sulfite reductase and the alpha subunit of adenosine-59-phosphosulfate reductase, respectively, produced results similar to those inferred from comparisons based on the 16S rRNA gene sequence. On the basis of phenotypic and phylogenetic data, strain Arc51 T represents a novel species of the genus Archaeoglobus, for which the name Archaeoglobus infectus sp. nov. is proposed. The type strain is Arc51 T (5NBRC 100649 T 5DSM 18877 T ).

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“…In both methanogenesis and epoxide carboxylation, reductive dealkylation of a CoM thioether occurs, in the former system to generate methane and in the latter to form an enolate that undergoes carboxylation (11,48,50). In addition to Xanthobacter and Rhodococcus, other organisms where CoM-dependent alkene metabolism has been identified and studied include strains of Mycobacterium (14), Nocardioides (41), Pseudomonas, and Ochrobactrum (15). Several strains of these microorganisms are able to grow on and degrade ethene and vinyl chloride in a monoxygenase-catalyzed reaction, yielding chlorooxirane and ultimately epoxyethane (14).…”

Section: Similarities In Com Utilization During Methanogenesis and Prmentioning

confidence: 99%

Getting a Handle on the Role of Coenzyme M in Alkene Metabolism

Krishnakumar

Sliwa

Endrizzi

et al. 2008

Microbiol Mol Biol Rev

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SUMMARY Coenzyme M (2-mercaptoethanesulfonate; CoM) is one of several atypical cofactors discovered in methanogenic archaea which participate in the biological reduction of CO2 to methane. Elegantly simple, CoM, so named for its role as a methyl carrier in all methanogenic archaea, is the smallest known organic cofactor. It was thought that this cofactor was used exclusively in methanogenesis until it was recently discovered that CoM is a key cofactor in the pathway of propylene metabolism in the gram-negative soil microorganism Xanthobacter autotrophicus Py2. A four-step pathway requiring CoM converts propylene and CO2 to acetoacetate, which feeds into central metabolism. In this process, CoM is used to activate and convert highly electrophilic epoxypropane, formed from propylene epoxidation, into a nucleophilic species that undergoes carboxylation. The unique properties of CoM provide a chemical handle for orienting compounds for site-specific redox chemistry and stereospecific catalysis. The three-dimensional structures of several of the enzymes in the pathway of propylene metabolism in defined states have been determined, providing significant insights into both the enzyme mechanisms and the role of CoM in this pathway. These studies provide the structural basis for understanding the efficacy of CoM as a handle to direct organic substrate transformations at the active sites of enzymes.

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Involvement of Coenzyme M during Aerobic Biodegradation of Vinyl Chloride and Ethene by Pseudomonas putida Strain AJ and Ochrobactrum sp. Strain TD

Cited by 34 publications

References 19 publications

Evaluating the fate of chlorinated ethenes in streambed sediments by combining stable isotope, geochemical and microbial methods

Evaluating the fate of chlorinated ethenes in streambed sediments by combining stable isotope, geochemical and microbial methods

Archaeoglobus infectus sp. nov., a novel thermophilic, chemolithoheterotrophic archaeon isolated from a deep-sea rock collected at Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean

Getting a Handle on the Role of Coenzyme M in Alkene Metabolism

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