Involvement of cyclic AMP, iodide and metabolites of arachidonic acid in the regulation of cell proliferation of isolated porcine thyroid follicles

Growth Regulation of the Human Papillary Thyroid Cancer Cell Line by Protein Tyrosine Kinase and cAMP-Dependent Protein Kinase.

“…TSH stimulated proliferation of a nontransformed rat cell line (FRTL; [10,29,30]) and the thyroid cells of rats [13] and dogs [14,15] in primary cultures. But no growth-promoting effects of TSH were demonstrated in primary thyroid cell culture from pig [16] or sheep [8]. Human thyroid cells were initially believed to be unresponsive to TSH [31], but in a recent study [32,33] TSH was found to be mitogenic for the normal thyroid cells in primary culture in the presence of IGF-1 or a high concentration of insulin.…”

“…It has been shown that basic fibroblast growth factor (FGF; [3,4]), epidermal growth factor (EGF; [r9]), and insulin-like growth factor-1 (IGF-1; [10][11][12]), all of which activate protein tyrosine kinase, stimulate proliferation of thyroid cells in cultures from various animal species. On the other hand, thyrotropin (TSH) which stimulates CAMP synthesis elicits different responses in thyroid cells in cultures from different animals [13][14][15][16].…”

Demonstration of the production and physiological role of insulin-like growth factor II in rat thyroid follicular cells in culture.

“…In recent years, regulation of thyroid cell growth has become a topic of interest in numerous laboratories, including our own (1)(2)(3)(4)(5)(6)(7)(8)(9). In pursuing this topic, we, like others, have employed as a model the FRTL5 line of rat thyroid follicular cells.…”

Pro-nucleotide Inhibitors of Adenylyl Cyclases in Intact Cells

“…TLC: R F ϭ 0.61 (chloroform/methanol ϭ 9:1, v/v). General Methods for Compound 6-1 H and 13 C NMR spectra were recorded at 400 MHz with proton decoupling, at ambient temperature, with a Brü ker DRX400. Chemical shifts are given in ␦ values referenced to the residual solvent peak: chloroform (CDCl 3 ) at 7.26 ppm and 77.6 ppm or dimethyl sulfoxide (Me 2 SO-d 6 ) at 2.49 ppm and 39.5 ppm, relative to tetramethylsilane.…”

“…Refs. [11][12][13][14][15][16][17][18]. Although cell-permeable, adenine nucleosides are much less potent than the corresponding nucleoside polyphosphates, and because of the relatively high concentrations needed to inhibit adenylyl cyclases may have unintended side effects (2-4, 6, 7, 11, 19 -24).…”