Involvement of G6PD5 in ABA response during seed germination and root growth in Arabidopsis

et al. 2021

IJMS

The BAG proteins are a family of multi-functional co-chaperones. In plants, BAG proteins were found to play roles both in abiotic and biotic stress tolerance. However, the function of Arabidopsis BAG2 remains largely unknown, whereas BAG6 is required for plants’ defense to pathogens, although it remains unknown whether BAG6 is involved in plants’ tolerance to abiotic stresses. Here, we show that both BAG2 and BAG6 are expressed in various tissues and are upregulated by salt, mannitol, and heat treatments and by stress-related hormones including ABA, ethylene, and SA. Germination of bag2, bag6 and bag2 bag6 seeds is less sensitive to ABA compared to the wild type (WT), whereas BAG2 and BAG6 overexpression lines are hypersensitive to ABA. bag2, bag6, and bag2 bag6 plants show higher survival rates than WT in drought treatment but display lower survival rates in heat-stress treatment. Consistently, these mutants showed differential expression of several stress- and ABA-related genes such as RD29A, RD29B, NCED3 and ABI4 compared to the WT. Furthermore, these mutants exhibit lower levels of ROS after drought and ABA treatment but higher ROS accumulation after heat treatment than the WT. These results suggest that BAG2 and BAG6 are negatively involved in drought stress but play a positive role in heat stress in Arabidopsis.

“…DAB and NBT staining were performed as previously described [ 46 ]. The experiment was performed three times and each time 20 seedlings of each genotype were stained.…”

Section: Methodsmentioning

confidence: 99%

The BAG2 and BAG6 Genes Are Involved in Multiple Abiotic Stress Tolerances in Arabidopsis Thaliana

Arif

et al. 2021

IJMS

“…H 2 O 2 acts as a signaling molecule and activates G6PDH activity to participate in plant resistance. Yang [39] found that G6PDH of CY type in Arabidopsis was closely related to ABA. The results of this experiment are consistent with previous studies, which prove that G6PDHs in strawberry can respond to ABA, and ABA as a signaling molecule participates in many stress processes, which may be one of the mechanisms of FaG6PDHs participating in stress.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Investigation of G6PDH Gene in Strawberry: Evolution and Expression Analysis during Development and Stress

Lei

Lin

et al. 2022

IJMS

As one of the key enzymes in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and plays an important role in plant development and stress responses. However, little information was available about the G6PDH genes in strawberry (Fragaria × ananassa). The recent release of the whole-genome sequence of strawberry allowed us to perform a genome-wide investigation into the organization and expression profiling of strawberry G6PDH genes. In the present study, 19 strawberry G6PDH genes (FaG6PDHs) were identified from the strawberry genome database. They were designated as FaG6PDH1 to FaG6PDH19, respectively, according to the conserved domain of each subfamily and multiple sequence alignment with Arabidopsis. According to their structural and phylogenetic features, the 19 FaG6PDHs were further classified into five types: Cy, P1, P1.1, P2 and PO. The number and location of exons and introns are similar, suggesting that genes of the same type are very similar and are alleles. A cis-element analysis inferred that FaG6PDHs possessed at least one stress-responsive cis-acting element. Expression profiles derived from transcriptome data analysis exhibited distinct expression patterns of FaG6PDHs genes in different developmental stages. Real-time quantitative PCR was used to detect the expression level of five types FaG6PDHs genes and demonstrated that the genes were expressed and responded to multiple abiotic stress and hormonal treatments.

“…DAB and NBT staining were performed as previously described [24]. The experiment was performed three times and each time 20 seedlings of each genotype were stained.…”

Section: Dab and Nbt Stainingmentioning

confidence: 99%

The BAG2 and BAG6 Genes are Involved in Multiple Abiotic Stress Tolerance in Arabidopsis Thaliana

Arif

et al. 2020

Preprint

Background: The BAG (Bcl-2 associated athanogene) family is a multi-functional group of proteins that perform functions ranges from apoptosis to environmental stress tolerance. The BAG proteins are found in yeast, plants and animals. In plants, especially in Arabidopsis thaliana (Arabidopsis), BAG proteins were found to play roles both in abiotic and biotic stresses tolerance including heat, cold, salt, drought, and pathogens. However, the function of Arabidopsis BAG2 remains largely unknown. Whereas BAG6 was found to play important roles in plants response to biotic stresses, it remains unknown whether BAG6 is involved in plants tolerance to abiotic stresses.Results: In this study, we have characterized functions of the Arabidopsis BAG2 and BAG6 genes. Promoter:GUS analysis results show that both BAG2 and BAG6 genes are expressed in various tissues in Arabidopsis thaliana. Expression of both BAG2 and BAG6 genes is induced by salt, mannitol, and heat stress treatments and by the stress-related hormones including ABA, ethylene, and SA, while BAG6 expression is additionally induced by JA. Germination of the bag2 and bag6 single and bag2 bag6 double mutant seeds are less sensitive to ABA than that of the wild type (WT). Both bag2 and bag6 single mutant and bag2 bag6 double mutant show more survival rate than WT in drought treatment but display less survival rate on 45 ℃ heat stress experiment. Consistently, transcription levels of stress-related genes such as RD29A, RD29B and NCED3 are higher in the mutant than that in the WT. Furthermore, these mutants exhibit lower content of reactive oxygen species (ROS) after drought and ABA treatment but higher ROS accumulation after heat treatment than the WT.Conclusion: These results suggest that BAG2 and BAG6 genes are negatively involved in drought stress but play a positive role in heat stress in Arabidopsis.