Involvement of gibberellin and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota)

Tokuji, Yoshihiko; Kuriyama, Kyoko

doi:10.1078/0176-1617-00892

Cited by 58 publications

(46 citation statements)

References 21 publications

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“…Some investigators have also suggested the necessity of polar auxin transport for initiation of embryo formation (Choi et al 1997(Choi et al , 2001Su and Zhang 2009). On the contrary, studies by Tokuji and Kuriyama (2003) showed that it is not needed in the induction of direct SE in carrot. Ramarosandratana and Van Staden (2004) hypothesised that the reduction of embryonal-suspensor mass initiation in Picea abies L. Karst.…”

Section: The Impact Of Biosynthetic Inhibitors On the Endogenous Hormmentioning

confidence: 99%

“…(Choi et al 2001) and for inducing stem cell formation within the embryonic callus of Arabidopsis (Su and Zhang 2009). Conversely, it does not appear to be required for the very early stages of direct SE in Daucus carota L. (Tokuji and Kuriyama 2003). Like auxin, cytokinins are key regulators for various aspects of plant growth and development, including somatic embryo production (Jiménez 2005).…”

Section: Introductionmentioning

confidence: 99%

“…However, their importance during the acquisition of embryogenic potential is poorly understood. Tokuji and Kuriyama (2003) showed that cytokinins regulate the early stage of SE in carrot. Somleva et al (1995) and Sáenz et al (2010) have found an inverse relationship between cytokinins and the embryogenic response.…”

Section: Introductionmentioning

confidence: 99%

“…By applying antiauxin or auxin polar transport inhibitors, many investigators have shown the necessity of this group of hormones for embryo maturation and its normal development (Vondráková et al 2016). It has been also proposed that auxin may act as a trigger in the embryogenic program of plant somatic cells (LoSchiavo et al 1989;Tokuji and Kuriyama 2003;Nic-Can and Loyla-Vargas 2016). Establishment of an auxin gradient was essential for initiating somatic embryos in Eleutherococcus senticosus Maxim.…”

Section: Introductionmentioning

confidence: 99%

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Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

2017

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Somatic embryogenesis (SE) in the tree fern Cyathea delgadii was first described in 2015 and since then has been used to exploration of this phenomenon in cryptogamic plants. To deepen the knowledge about the hormonal control of SE, stipe explants were cultured on media supplemented with hormone biosynthesis and transport inhibitors (HBTIs). In the presence of 30 µM 2,3,5-triiodobenzoic acid (TIBA), or 40 µM fluridone or 125 µM salicylic acid (SA), somatic embryo production was totally inhibited. The quantitative analysis of the changes in endogenous hormone and sugar contents was conducted every 2 days within 10-day-long initial culture. The results showed that the concentrations of endogenous indole-3-acetic acid (IAA), abscisic acid (ABA), cytokinins (CKs) and soluble sugars were strongly modified either by TIBA and fluridone. Under their influence, the contents of cytokinins such as c-Z, c-ZR, t-Z, t-ZR, KinR were reduced to barely detectable levels. Treatment with SA results in the changes in endogenous IAA and sugar contents. It also modifies the IAA/CKs ratio; however, excluding the first 2 days of culture, the concentrations of ABA and cytokinins were kept on the control level. All HBTIs significantly increased the kinetin (Kin) content. Our work sheds new light on the relationships between the biosynthetic inhibitors and phytohormones and sugars in the process of early SE. It can be helpful to study the role of hormones in acquisition of embryogenic competence.

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Section: The Impact Of Biosynthetic Inhibitors On the Endogenous Hormmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

2017

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“…Furthermore, consistent with their roles in zygotic embryogenesis, the ABA-to-GA ratio is also known to affect somatic embryogenesis (SE). ABA treatments promote SE, while GA has an antagonistic role in SE in carrot (Daucus carota) cells (Tokuji and Kuriyama, 2003;Kikuchi et al, 2006). In addition, in many instances, seed dormancy can be released by applying fluridone, an inhibitor of carotenoid synthesis that causes a decrease in endogenous ABA levels (Yoshioka et al, 1998;Grappin et al, 2000).…”

mentioning

confidence: 99%

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

et al. 2014

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Kalanchoë daigremontiana reproduces asexually by generating numerous plantlets on its leaf margins. The formation of plantlets requires the somatic initiation of organogenic and embryogenic developmental programs in the leaves. However, unlike normal embryogenesis in seeds, leaf somatic embryogenesis bypasses seed dormancy to form viable plantlets. In Arabidopsis (Arabidopsis thaliana), seed dormancy and embryogenesis are initiated by the transcription factor LEAFY COTYLEDON1 (LEC1). The K. daigremontiana ortholog of LEC1 is expressed during leaf somatic embryo development. However, KdLEC1 encodes for a LEC1-type protein that has a unique B domain, with 11 unique amino acids and a premature stop codon. Moreover, the truncated KdLEC1 protein is not functional in Arabidopsis. Here, we show that K. daigremontiana transgenic plants expressing a functional, chimeric KdLEC1 gene under the control of Arabidopsis LEC1 promoter caused several developmental defects to leaf somatic embryos, including seed dormancy characteristics. The dormant plantlets also behaved as typical dormant seeds. Transgenic plantlets accumulated oil bodies and responded to the abscisic acid biosynthesis inhibitor fluridone, which broke somatic-embryo dormancy and promoted their normal development. Our results indicate that having a mutated form of LEC1 gene in K. daigremontiana is essential to bypass dormancy in the leaf embryos and generate viable plantlets, suggesting that the loss of a functional LEC1 promotes viviparous leaf somatic embryos and thus enhances vegetative propagation in K. daigremontiana. Mutations resulting in truncated LEC1 proteins may have been of a selective advantage in creating somatic propagules, because such mutations occurred independently in several Kalanchoë species, which form plantlets constitutively.

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Participation of Plant Hormones in Determination and Progression of Somatic Embryogenesis

García¹,

Thomas²

Plant Cell Monographs

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Involvement of gibberellin and cytokinin in the formation of embryogenic cell clumps in carrot (Daucus carota)

Cited by 58 publications

References 21 publications

Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

Effect of TIBA, fluridone and salicylic acid on somatic embryogenesis and endogenous hormone and sugar contents in the tree fern Cyathea delgadii Sternb.

Truncation of LEAFY COTYLEDON1 Protein Is Required for Asexual Reproduction in Kalanchoë daigremontiana

Participation of Plant Hormones in Determination and Progression of Somatic Embryogenesis

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