Involvement of glycolate metabolism in acclimation of Chlorella vulgaris cultures to low phosphate supply

Kozlowska-Szerenos, B.; Zieliński, Piotr

doi:10.1016/s0981-9428(00)01175-x

Cited by 25 publications

(12 citation statements)

References 31 publications

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“…Beardall et al (2005) suggested that CCM activity of Chlorella emersonii was down-regulated by P limitation. On the contrary, the phosphorus-deficient cells of Chlorella vulgaris showed increased inorganic carbon affinity (Kozłowska-Szerenos et al 2000), which was in accordance with our results. It has been demonstrated that the effect of phosphate on carbon metabolism may depend also on other factors, especially on irradiance and CO 2 concentration.…”

Section: Resultssupporting

confidence: 92%

“…Since carbon acquisition in most microalgae is energy dependent, requiring ATP, it might be expected that phosphate limitation could have an indirect regulatory influence on CCM activity (Beardall et al 1998). The CCM activity of Chlorella emersonii was down-regulated by phosphate limitation , however, phosphate deficiency increased the affinity for external inorganic carbon (1/K 0.5 ) in photosynthesis of Chlorella vulgaris at high irradiance (Kozłowska-Szerenos et al 2000, 2004.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Zhou

2009

World J Microbiol Biotechnol

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Nannochloropsis sp. was grown to the exponential phase and transferred to the high CO 2 (2,800 ll l -1 ) and irradiance (100 lmol photons m -2 s -1 ) condition with different levels of nitrate and phosphate for 72 h, then the photosynthetic activity and inorganic carbon acquisition of the alga were measured. The apparent photosynthetic efficiency (a) of Nannochloropsis sp. decreased with increasing NO 3 -concentration from 150 to 3,000 lM, and the high nitrate-grown cells showed the lowest levels of light-saturated photosynthetic rate (P m ), while the low nitrate-grown cells showed the highest levels of dark respiration rate (R d ). The maximal light-saturated photosynthetic rate and the minimal dark respiration rate were seen under the middle nitrate condition. When the nitrate concentration ranged from 150 to 3,000 lM, the affinity for inorganic carbons of Nannochloropsis sp. increased sharply with the increasing NO 3 -concentration to 300 lM and then decreased significantly. The middle phosphate-grown cells exhibited the highest light-saturated photosynthetic rate and apparent photosynthetic efficiency, however, the affinity for inorganic carbons of Nannochloropsis sp. was the maximum under the low phosphate condition. It was shown that the appropriate nitrogen and phosphorus levels were of vital importance to the photosynthesis of cells.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Zhou

2009

World J Microbiol Biotechnol

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“…The increase in T ( see UVR × T + treatment) was the main factor that reduced the damaging UVR effect on PP in La Caldera, through the disposing of the surplus fixed C (EOC rate and %EOC). In fact, the involvement of glycolate metabolism (main constituent of EOC) has been described as mechanism to protect chloroplasts against photoinhibitory damage by the consumption of excess absorbed light energy (Kozlowska‐Szerenos et al ). This response could be interpreted as one possible pathway to maintain the Mehler reaction (Radmer and Kok ), leading to the reduction of an inhibitory UVR effect on PP.…”

Section: Discussionmentioning

confidence: 99%

Changes in the phytoplankton-bacteria coupling triggered by joint action of UVR, nutrients, and warming in Mediterranean high-mountain lakes

et al. 2015

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From an extensive study, we determined that heterotrophic bacterial production (HBP) variance in Sierra Nevada (Spain) lakes was explained mainly by excretion of organic carbon by algae (EOC), underlining a bacterial dependence on algal carbon. Subsequently, we studied how the interaction among global change factors such as ultraviolet radiation (UVR), nutrient inputs, and increased temperature affected this phytoplankton-bacteria coupling through in situ factorial experiments in two model high-mountain lakes, La Caldera, and Las Yeguas. Bacterioplankton were more sensitive than phytoplankton because the joint action of increased temperature and nutrient-addition unmasked an inhibitory UVR effect on HBP while reducing the inhibitory UVR effect on primary production (PP) (in La Caldera) or augmenting the net PP values (in Las Yeguas). The interaction among the three factors had a different effect on phytoplankton-bacteria coupling depending on the lake. Thus, in the colder lake (La Caldera), EOC was not adequate to meet the bacterial carbon demand (BCD), leading to a mismatch in phytoplankton-bacteria coupling. Contrarily, in the warmer lake (Las Yeguas), the phytoplankton-bacteria coupling was accentuated by the interaction among the three factors, with EOC exceeding BCD. These contrasting responses of phytoplankton-bacteria coupling may affect the microbial loop development, becoming reinforced in warmer and less UVR-transparent highmountain lakes, but weakened in colder and more UVR-transparent high-mountain lakes, with implications in the C-flux of these sentinel ecosystems in a scenario of global change.

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“…An estimation of C i affinity of high-and low-CO 2 -growing cells was done according to Kozłowska-Szerenos et al (2000). Cells were harvested by centrifugation (1,200g, 10 min), resuspended in 25 mM HEPES-KOH to a constant OD 750 of 0.35 6 0.05, and transferred into the electrode chamber.…”

Section: Measurement Of Cell Affinity For Dissolved C Imentioning

confidence: 99%

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

et al. 2010

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(J.A.R.) Using a gas chromatography-mass spectrometry-time of flight technique, we determined major metabolite changes during induction of the carbon-concentrating mechanism in the unicellular green alga Chlamydomonas reinhardtii. In total, 128 metabolites with significant differences between high-and low-CO 2 -grown cells were detected, of which 82 were wholly or partially identified, including amino acids, lipids, and carbohydrates. In a 24-h time course experiment, we show that the amino acids serine and phenylalanine increase transiently while aspartate and glutamate decrease after transfer to low CO 2 . The biggest differences were typically observed 3 h after transfer to low-CO 2 conditions. Therefore, we made a careful metabolomic examination at the 3-h time point, comparing low-CO 2 treatment to high-CO 2 control. Five metabolites involved in photorespiration, 11 amino acids, and one lipid were increased, while six amino acids and, interestingly, 21 lipids were significantly lower. Our conclusion is that the metabolic pattern during early induction of the carbon-concentrating mechanism fit a model where photorespiration is increasing.

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Involvement of glycolate metabolism in acclimation of Chlorella vulgaris cultures to low phosphate supply

Cited by 25 publications

References 31 publications

Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Regulation of inorganic carbon acquisition by nitrogen and phosphorus levels in the Nannochloropsis sp.

Changes in the phytoplankton-bacteria coupling triggered by joint action of UVR, nutrients, and warming in Mediterranean high-mountain lakes

A Metabolomic Approach to Study Major Metabolite Changes during Acclimation to Limiting CO2 in Chlamydomonas reinhardtii

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