Involvement of ionotropic glutamate receptors in low frequency electroacupuncture analgesia in rats

Choi, Byung-Tae; Lee, Jun‐Hyuk; Wan, You; Han, Ji-Sheng

doi:10.1016/j.neulet.2004.11.095

Cited by 27 publications

(17 citation statements)

References 15 publications

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“…It is also well known that EA shows anti-hyperalgesic effects that have been confirmed in previous clinical and animal experimental studies. NMDARs may also be involved in the induction of EA analgesia in the spinal cord of normal animals (11), which indicates that any changes in its activation Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model may influence EA analgesia. Combined treatment with EA and NMDAR antagonists has a synergistic antinociceptive effect in inflammatory pain models (12,13).…”

Section: Introductionmentioning

confidence: 99%

Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model

Choi¹

2011

Int J Mol Med

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Abstract. This study examined the synergistic antinociceptive effects associated with signaling pathway proteins of the spinal cord in a complete Freund's adjuvant (CFA)-induced pain model when electroacupuncture (EA) and a N-methyl-D-aspartate receptor (NMDAR) antagonist were administered in combination. EA stimulation (2 Hz, 1 mA) was needledelivered for 20 min once daily at acupoints corresponding to Zusanli and Sanyinjiao with intrathecal injection of the NMDAR antagonist dizocilpine (MK801). Thermal sensitivity of the hindpaw induced by CFA was strongly inhibited by dizocilpine injection and EA stimulation. Co-treatment with EA and dizocilpine showed a synergistic antinociceptive effect against inflammatory pain. On day two of the experiment, we examined the phosphorylation of the NMDAR NR2B subunit, of the extracellular signal-regulated kinase (ERK), p38 and of the cAMP response element-binding protein (CREB) in the ipsilateral dorsal horn of L4-5 segments by Western blot analysis. Phosphorylation of the NMDAR NR2B subunit induced by CFA was markedly inhibited by co-treatment with dizocilpine and EA, but not by dizocilpine or EA treatment alone. CFA-induced phosphorylation of the ERK was inhibited by both dizocilpine and EA, but that of p38 was inhibited by EA only. CFA-induced phosphorylation of CREB was inhibited by dizocilpine, but did not show marked changes. Immunohistochemical analyses confirmed that there was a significant difference in the NMDAR NR2B subunit and ERK phosphorylation. It is possible that the combined treatment with EA and the NMDAR antagonist dizocilpine resulted in synergistic antinociceptive effects in an inflammatory pain model via the inactivation of both the NMDAR NR2B subunit and ERK of the spinal cord.

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Section: Introductionmentioning

confidence: 99%

Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model

Choi¹

2011

Int J Mol Med

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“…In the study of the mechanisms of acupuncture analgesia, Dr. Han and his colleagues [63,64] at The School of Basic Medical Sciences of Peking University found that while the opioid system and its regulatory system, such as cholecystokinin and its receptors, are factors, excitatory amino acids are also involved in mediating acupuncture analgesia [65][66][67][68]. Long-term synaptic plasticity in the dorsal spinal cord is involved in frequencydependent electroacupuncture analgesia [69].…”

Section: Mechanisms For Acupuncture Analgesiamentioning

confidence: 99%

Pain research in China

Zhang

2010

Sci. China Life Sci.

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In addition to investigating the anatomy, neurochemistry and neurophysiology of pain pathways, Chinese researchers have extended their work into the molecular and cellular mechanisms of sensory afferent transmission at the spinal cord level as well as cognitive processing in the brain. The mechanism underlying acupuncture analgesia remains a subject of special interest for Chinese pain researchers, with the aim of combining clinical practice with the understanding of pain transmission and analgesic mechanism.

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“…In a recent study of the neurochemical mechanisms of acupuncture analgesia, we found that: (1) while opioid systems (Han 2003(Han , 2004) play a major role in mediating acupuncture analgesia, excitatory amino acids are also heavily involved (Choi et al 2005;Huang et al 2005); (2) the effect of acupuncture analgesia was observed not only in normal rats, but also in rat models of chronic pain, including neuropathic pain Huang et al 2004a) and of inflammatory pain (Huang et al 2004b;Liu HX et al 2007;Liu FY et al 2007); (3) in order to analyze the genetic background of AA, we started to use mice instead of rats. The neurochemical mechanisms underlying AA in rats were also found in mice, including opioids and nocistatin (Huang et al 2003), cholecystokinin B (CCK B ) receptor system ), etc.…”

Section: Pain Analgesia and Acupuncture Analgesiamentioning

confidence: 99%

The Neuroscience Research Institute at Peking University: A Place for the Solution of Pain and Drug Abuse

Wan

Chui

et al. 2007

Cell Mol Neurobiol

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Neuroscience research in China has undergone rapid expansion since 1980. The Neuroscience Research Institute of Peking University, one of the most active neuroscience research groups in China, was founded in 1987. Currently, the institute is overseeing four research areas, i.e., (1) pain and analgesia, (2) drug abuse and acupuncture treatment for drug addiction, (3) the mechanism of neurological degenerative disorders, and (4) the role of neuroglia in central nervous system injury. The institute is simultaneously investigating both theoretical and clinical studies. Acupuncture remains the core of research, while pain and drug abuse form the two disciplines.

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Involvement of ionotropic glutamate receptors in low frequency electroacupuncture analgesia in rats

Cited by 27 publications

References 15 publications

Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model

Synergistic antinociceptive effects of N-methyl-D-aspartate receptor antagonist and electroacupuncture in the complete Freund's adjuvant-induced pain model

Pain research in China

The Neuroscience Research Institute at Peking University: A Place for the Solution of Pain and Drug Abuse

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