Involvement of M cells in the bacterial invasion of Peyer's patches: a common mechanism shared by Yersinia enterocolitica and other enteroinvasive bacteria.

Grützkau, Andreas; Hanski, C.; Hahn, Helmut; Riecken, Ernst–Otto

doi:10.1136/gut.31.9.1011

Cited by 243 publications

(143 citation statements)

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“…Although there are some reports on the involvement of M cells in the initial step of the infection with enteric pathogens [1][2][3][4][5], the formation of the pseudopodium as big as that demostrated in the present study is not known. Therefore, the pseudopodia may have been developed specifically for uptake of the killed bacteria.…”

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confidence: 67%

“…A thinner pseudopodium was observed in M It has been shown that microfold (M) cells in the epithelium of the Peyer's patches dome region are involved in the initial stage of the invasion of enteropathogens [2,8,11]. An association of M cells with the intestinal invasion of Yersinia known as an agent of human gastroenteritis is known [1,[3][4][5]. The mechanism of the invasion of these pathogens into the M cells, however, is still obscure.…”

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confidence: 99%

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Uptake of Killed Yersinia enterocolitica by Pseudopodia of M Cells in the Peyers' Patches of the Murine Small Intestines.

Kaneko

Uehara

Ogawa

1999

The Journal of Veterinary Medical Science

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ABSTRACT. To understand the mechanisms of uptake of killed bacteria of Yersinia enterocolitica serovar O3 into the epithelium of Peyer's patches, the killed bacteria were perorally inoculated into mice and observation was carried out by scanning and transmission electron microscopy. Microfold (M) cells formed a specific pseudopodium-like structure and the bacteria were observed on the surface and the interior of the pseudopodium-like structure 8 hr after oral administration of killed bacteria of serovar O3.-KEY WORDS: M cell, pseudopodium, Yersinia enterocolitica.J. Vet. Med. Sci. 61(10): 1175-1177, 1999 excised from four mice under anesthesia with ethyl ether 8 and 24 hr after oral inoculation and from two control mice. The small intestines excised were rinsed in PBS. The Peyer's patches were collected from the small intestines and soaked in 2.5% glutaraldehyde 10 min after excision of the small intestines. After overnight fixation with glutaraldehyde, the specimens were fixed with osmic acid according to the routine method. The specimens were washed, dehydrated through a graded series of ethanol and transferred to isoamyl acetate for scanning electron microscopy. After critical-point drying, the specimens were coated with gold with an ion sputtering apparatus and observed under a scanning electron microscope (JEOL T330A) at 15 kV. For transmission electron microscopy, the specimens dehydrated were embedded in Epon. Ultrathin (70 to 80 nm) sections were stained with uranyl acetate and lead citrate and examined under a JEOL 100C transmission electron microscope. Figure 1 shows M cells in the epithelium of the Peyer's patches 8 and 24 hr after oral administration of serovar O3 killed bacteria. M cells formed a specific structure like a pseudopodium and the bacteria were observed on the surface 8 hr after the administration. Some bacteria were in close contact with the surface and some were embedded in the specific structure of M cells. Although the bacteria were not observed, M cells were still forming the specific pseudopodium-like structure 24 hr after the administration. On the other hand, the pseudopodium-like structure was not observed in the control mice not given the killed serovar O3 bacteria. Figure 2 shows M cells in the epithelium of the Peyer's patches 8 hr after the oral administration. The killed bacteria were still in the lumen of the small intestines. M cells formed the same specific structure as that observed by scanning electron microscopy. The killed bacteria were observed on the surface and the interior of the pseudopodium.The bacteria were taken up only by the pseudopodium of the M cell. The killed bacteria were not seen in microvilli of the M cell. A thinner pseudopodium was observed in M It has been shown that microfold (M) cells in the epithelium of the Peyer's patches dome region are involved in the initial stage of the invasion of enteropathogens [2,8,11]. An association of M cells with the intestinal invasion of Yersinia known as an agent of human gastroenteritis is known [1,[3][4][5]. ...

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confidence: 67%

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Uptake of Killed Yersinia enterocolitica by Pseudopodia of M Cells in the Peyers' Patches of the Murine Small Intestines.

Kaneko

Uehara

Ogawa

1999

The Journal of Veterinary Medical Science

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“…After intestinal colonization by pathogenic Yersinia, the bacteria penetrate the intestinal mucosa through the M cells. After penetration, Yersinia colonize Peyer's patches, possibly disseminating through the lymphatic pathway and eventually reaching the mesenteric lymph nodes, the liver, and the spleen (Grutzkau et al 1990, Falkow et al 1992, Clark et al 1998.…”

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Experimental kinetics of infection induced by Yersinia pseudotuberculosis isolated from stock animals

Martins

Falcão

2004

Mem. Inst. Oswaldo Cruz

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“…The bacteria were in direct contact with Yersinia enterocolitica is an agent of human gastroenteritis. Experimental infection has been induced to clarify the mechanisms of intestinal colonization in mice by using mainly Y. enterocolitica serovar O8 which causes systemic infection [2,[5][6][7][8]. Carter [2] reported that serovar O8 colonized in the mouse Peyer'spatches.…”

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“…Grutzkau et al [5] and Autenrieth and Firsching [1] also suggested the involvement of M cells in the initial stage of intestinal colonization of serovar O8 from the evidence of serovar O8 in contact with and penetrating into the M cells. Although Grutzkau et al [5] described phagocytosis by M cells and observed a small immature pseudopodium-like structure, uptake of bacteria by the pseudopodium of M cells has not been demonstrated.…”

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confidence: 99%

Morphology of Intestinal Colonization of Yersinia enterocolitica Serovar O3 in Mice.

Kaneko

Uehara

Hayashidani

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. The present study was made to know the morphology of the initial invasion and lesions involved in the intestinal colonization of Yersinia enterocolitica serovar O3 in the epithelium of Peyer's patches of mice. Microfold (M) cells formed a specific structure like a pseudopodium and the bacteria were observed on the surface of the pseudopodium-like structure 4 hr after oral administration of serovar O3. The colonies of serovar O3 were observed in the epithelium and the lamina propria of the Peyer's patches dome region, and the bacteria grown in the Peyer's patches were in direct contact with the lumen without covered with the host tissue 24 hr after the administration.-KEY WORDS: M cell, Peyer's patch, Yersinia enterocolitica.J. Vet. Med. Sci. 61(10): 1171-1173, 1999 The sections were allowed to react at room temperature for an hour with anti-O3 rabbit sera made in our laboratory, washed three times with PBS, then treated with peroxidaselabelled anti-rabbit IgG-goat antibody (KPL) at room temperature for an hour. The sections were fixed with 1% glutaraldehyde, washed three times with PBS and treated with 0.05 M tris buffer pH 7.6 containing 0.04% 3,3'-diamino-benzidine and 0.005% H 2 O 2 for 10 min. Counter-staining was made with a 1% methyl green solution. The sections were dehydrated and observed under an optical microscope according to the routine methods.Specimens for a scanning electron microscopy were obtained by the same method of the optical microscopy as described above. The specimens obtained were fixed with glutaraldehyde and osmic acid. They were washed, dehydrated through a graded series of ethanol, and transferred to isoamylacetate. After critical-point drying, the specimens were coated with gold with an ion sputtering apparatus and observed under a scanning electronmicroscope (JEOL T330A) at 15 kV.Although small colonies were observed in the subepithelial region 8 hr after oral administration as shown in Fig. 1A, no such colonies were found in the sections obtained from specimens 4 hr after oral administration.The colonies of serovar O3 were observed in the epithelium and the lamia propria of only the dome area in the Peyer's patches. No colonies were observed in the regions of the intestinal villi or the center of Peyer's patches as shown in Fig. 1B. Figure 2A shows the surface of the dome region of Peyer's patches of the small intestines 4 hr after oral administration, a structure like a pseudopodium formed from the microfold (M) cells was observed and the bacteria were seen on the surface of the pseudopodium-like structure. M cells in the control mice on the other hand, were forming nothing like a pseudopodium (Fig. 2B). Figure 3 shows that the epithelium of the dome has been destroyed 24 hr after oral administration due to the growth of serovar O3. The bacteria forming colonies were seen without covered with the cell membrane or other substance of the host tissue. The bacteria were in direct contact with Yersinia enterocolitica is an agent of human gastroenteritis. Experimental infecti...

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Involvement of M cells in the bacterial invasion of Peyer's patches: a common mechanism shared by Yersinia enterocolitica and other enteroinvasive bacteria.

Cited by 243 publications

References 22 publications

Uptake of Killed Yersinia enterocolitica by Pseudopodia of M Cells in the Peyers' Patches of the Murine Small Intestines.

Uptake of Killed Yersinia enterocolitica by Pseudopodia of M Cells in the Peyers' Patches of the Murine Small Intestines.

Experimental kinetics of infection induced by Yersinia pseudotuberculosis isolated from stock animals

Morphology of Intestinal Colonization of Yersinia enterocolitica Serovar O3 in Mice.

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