Involvement of mast cells in a mouse model of postoperative pain

Oliveira, Sara Marchesan; Drewes, Carine Cristiane; Silva, Cássia Regina; Trevisan, Gabriela; Boschen, Suelen Lúcio; Moreira, Camila Guimarães; Cabrini, Daniela de Almeida; Cunha, Cláudio Da; Ferreira, Juliano

doi:10.1016/j.ejphar.2011.10.001

Cited by 69 publications

(71 citation statements)

References 61 publications

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“…Neutrophils were counted in 3 hind paws per treatment from the heel to the toes in adjacent fields of view at 400X magnification (A). Mast cells were counted in 3 footpads/treatment and their degranulation status was scored based on the number of visible granules outside of the cell boundary: intact (0), mild (0-10), moderate (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20), extensive (21+), as described previously 10 (B). To avoid counting the same cells twice, the investigators assessing neutrophil numbers and mast cell degranulation levels in the hind paw sections used a grid inscribed in the eyepiece reticle.…”

Section: Representative Resultsmentioning

confidence: 99%

“…Reduction in time and intensity of previously specified responses to noxious stimuli are used to measure pain states in these animals as rodent subjects are not otherwise able to report a sensation of pain 6 . Altered responses to temperature and pressure are commonly used as metrics of tissue sensitivity in rodent models of inflammatory nociception that can be caused by infection 7,8 , chemical exposure 6,9,10 , surgical incision 11 , as well as neuropathic pain caused by nerve ligation 12 …”

Section: Introductionmentioning

confidence: 99%

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Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus

Martinov

Mack

Sykes

et al. 2013

JoVE

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Measuring inflammation-induced changes in thresholds of hind paw withdrawal from mechanical pressure is a useful technique to assess changes in pain perception in rodents. Withdrawal thresholds can be measured first at baseline and then following drug, venom, injury, allergen, or otherwise evoked inflammation by applying an accurate force on very specific areas of the skin. An electronic von Frey apparatus allows precise assessment of mouse hind paw withdrawal thresholds that are not limited by the available filament sizes in contrast to classical von Frey measurements. The ease and rapidity of measurements allow for incorporation of assessment of tactile sensitivity outcomes in diverse models of rapid-onset inflammatory and neuropathic pain as multiple measurements can be taken within a short time period. Experimental measurements for individual rodent subjects can be internally controlled against individual baseline responses and exclusion criteria easily established to standardize baseline responses within and across experimental groups. Thus, measurements using an electronic von Frey apparatus represent a useful modification of the well-established classical von Frey filament-based assays for rodent mechanical allodynia that may also be applied to other nonhuman mammalian models.

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Section: Representative Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus

Martinov

Mack

Sykes

et al. 2013

JoVE

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“…In the bladder in IC/PBS patients and in other tissues in other inflammatory disorders, mast cells accumulate mostly at the tips of nerve endings (33,41,56). Mast cells have been shown to play an important role in mediating pain in animal models of diseases such as IC/PBS, irritable bowel syndrome, and neuropathic and postoperative pain (40,47,49,57). Also, histamine released from mast cells has been shown to mediate pelvic pain in a virus-induced neurogenic cystitis model (45).…”

mentioning

confidence: 99%

Chronic pelvic allodynia is mediated by CCL2 through mast cells in an experimental autoimmune cystitis model

Bicer

Altuntas

İzgi

et al. 2015

American Journal of Physiology-Renal Physiology

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The cause of chronic pelvic pain in interstitial cystitis/painful bladder syndrome (IC/PBS) remains unclear; autoimmunity is a possible etiology. We have recently shown that injection of a single immunogenic peptide of uroplakin 3A (UPK3A 65-84) induces experimental autoimmune cystitis (EAC) in female BALB/cJ mice that is unique among experimental models in accurately reflecting both the urinary symptoms and pelvic pain of IC/PBS. The aim of this project was to identify the roles of mast cells and mast cell chemoattractant/activator monocyte chemoattractant protein-1 [chemokine (C-C motif) ligand 2 (CCL2)] in the allodynia in this model. We immunized 6- to 8-wk-old female BALB/cJ mice with UPK3A 65-84 peptide and, 5–40 days later, observed increased responses to stimulation of the suprapubic abdominal and hindpaw surfaces with von Frey monofilaments compared with mice injected with adjuvant alone. Suprapubic and hindpaw tactile allodynia responses by EAC mice were blocked by instillation of lidocaine into the bladder but not by lidocaine in the uterus, confirming the bladder as the source of the hypersensitivity. Markedly increased numbers of activated mast cells and expression of CCL2 were found in the bladder after immunization with UPK3A 65-84. Hypersensitive responses were inhibited by mast cell stabilizer cromolyn sodium and antagonists of histamine receptors 1 and 2. Furthermore, BALB/cJ mice with deletion of the Ccl2 or chemokine (C-C motif) receptor 2 gene exhibited markedly reduced allodynia and accumulation of mast cells after UPK3A 65-84 immunization. These results show that UPK3A 65-84 immunization causes chronic visceral allodynia and suggest that it is mediated by CCL2-driven mast cell accumulation in the bladder.

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“…An interesting paper by Oliveira et al (30) aimed to investigate the involvement of mast cells in a model of postoperative pain in mice. Plantar surgery produced immense mast cell degranulation, and a pre-treatment with antihistamines decreased postoperative nociception in mice.…”

Section: Discussionmentioning

confidence: 99%

“…Also, histamine, an inflammatory mediator, is capable of sensitizing and activating nociceptive sensory nerve fibres (29). Oliveira et al (30) demonstrated that pre-treatment with antihistamines decreased postoperative nociception in mice. However, there is no study related to the effect of preoperative oral administration of antihistamine on postoperative pain.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Preoperative Administration of Antihistamine, Analgesic and Placebo on Postoperative Pain in Teeth with Symptomatic Apical Periodontitis: A Randomized Controlled Trial

Arslan¹,

Gundogdu²

2017

Eur Endod J

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Objective:To compare the effect of preoperative administration of an antihistamine, an analgesic, or a placebo on postoperative pain in maxillary molar teeth with symptomatic apical periodontitis. Design: A randomized, controlled trial. Methods: Population: A total of 51 patients with moderate-to-severe maxillary molar tooth pain who have undergone a root canal treatment. Intervention: Preoperative administration of either an antihistamine or an analgesic. Control: Versus placebo Outcomes: Reduction in postoperative pain? The data were analyzed with chi-square, one-way analysis of variance (ANOVA) and least significant difference (LSD) post-hoc tests. Results: At days 1 and 3, preoperative administration of the antihistamine and analgesic resulted in lower pain levels than the placebo. At days 5 and 7, however, while preoperative administration of the antihistamine still resulted in less pain than the placebo, there was no significant difference between the analgesic and placebo (P>0.05). Conclusion: Within the limitations of the present study, the preoperative administration of an antihistamine can be beneficial in reducing the postoperative pain of endodontic treatments.

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Involvement of mast cells in a mouse model of postoperative pain

Cited by 69 publications

References 61 publications

Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus

Measuring Changes in Tactile Sensitivity in the Hind Paw of Mice Using an Electronic von Frey Apparatus

Chronic pelvic allodynia is mediated by CCL2 through mast cells in an experimental autoimmune cystitis model

The Effect of Preoperative Administration of Antihistamine, Analgesic and Placebo on Postoperative Pain in Teeth with Symptomatic Apical Periodontitis: A Randomized Controlled Trial

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