Involvement of mitogen-activated protein kinase activation in cyclooxygenase-2 and transforming growth factor-β production in alveolar macrophage from chronic bronchitis rats

Huang, Yan; Gao, Jike; Meng, Xianli; Jiang, GL; Yang, Yunyun; Li, H; Liu, J; Li, J

doi:10.3109/08923973.2011.557383

Cited by 6 publications

(5 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They were allowed 3 days to adjust to the facilities before experimentation. Chronic bronchitis model was induced by BCG treatment (2.5 mL/kg) through the caudal vein, and LPS treatment (1 mg/kg) through endotracheal instillation 1 week later [ 7 ]. Then, the animals were randomly divided into six groups ( n = 10): the control group (Con), the model group (Mod), the Dex group, and the high, middle, and low CBPP dose groups (CBPP-H, CBPP-M, CBPP-L).…”

Section: Methodsmentioning

confidence: 99%

An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills for pulmonary diseases

Jin

Hou

et al. 2015

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundTraditional Chinese medicine (TCM) herbal formulae provide valuable therapeutic strategies. However, the active ingredients and mechanisms of action remain unclear for most of these formulae. Therefore, the identification of complex mechanisms is a major challenge in TCM research.MethodsThis study used a network pharmacology approach to clarify the anti-inflammatory and cough suppressing mechanisms of the Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills (ChuanbeiPipa dropping pills, CBPP). The chemical constituents of CBPP were identified by high-quality ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS), and anti-inflammatory ingredients were selected and analyzed using the PharmMapper and Kyoto Encyclopedia of Genes and Genomes (KEGG) bioinformatics websites to predict the target proteins and related pathways, respectively. Then, an RNA-sequencing (RNA-Seq) analysis was carried out to investigate the different expression of genes in the lung tissue of rats with chronic bronchitis.ResultsSix main constituents affected 19 predicted pathways, including ursolic acid and oleanolic acid from Eriobotrya japonica (Thunb.) Lindl. (Eri), peiminine from Fritillaria usuriensis Maxim. (Fri), platycodigenin and polygalacic acid from Platycodon grandiflorum (Jacq.) A. DC. (Pla) and guanosine from Pinellia ternata (Thunb.) Makino. (Pin). Expression of 34 genes was significantly decreased after CBPP treatment, affecting four therapeutic functions: immunoregulation, anti-inflammation, collagen formation and muscle contraction.ConclusionThe active components acted on the mitogen activated protein kinase (MAPK) pathway, transforming growth factor (TGF)-beta pathway, focal adhesion, tight junctions and the action cytoskeleton to exert anti-inflammatory effects, resolve phlegm, and relieve cough. This novel approach of global chemomics-integrated systems biology represents an effective and accurate strategy for the study of TCM with multiple components and multiple target mechanisms.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0983-y) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills for pulmonary diseases

Jin

Hou

et al. 2015

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…23 In inflammatory conditions, the MAPK signaling pathway overactivates M1 macrophages while stimulating the secretion of large amounts of inflammatory cytokines, which may contribute to the development of COPD. [24][25][26][27] The expression of p38, ERK, and JNK is influenced by a variety of factors, with m6A RNA methylation being one of the most common post-transcriptional modifications documented in a variety of inflammatory diseases. 28 Consistent with our findings, m6A regulators may be involved in the inflammatory response induced by M1 macrophage hyperactivation by regulating the MAPK signaling pathway, thereby participating in the development of COPD.…”

Section: Discussionmentioning

confidence: 99%

The Activation of M1 Macrophages is Associated with the JNK-m6A-p38 Axis in Chronic Obstructive Pulmonary Disease

Hu,

Pang,

et al. 2023

COPD

View full text Add to dashboard Cite

Background Excessive activation of M1 macrophages affects the chronic inflammatory response of the airways and leads to the development of chronic obstructive pulmonary disease (COPD). Therefore, it needs to be closely monitored and investigated. MAPK signaling pathway is involved in the activation of M1 macrophages, and N6-methyladenosine (m6A) is involved in the pathogenesis of COPD. However, it is unknown whether activation of the MAPK signaling pathway is mediated by m6A in M1 macrophages in COPD. Methods The GEO data were analyzed using bioinformatics techniques to assess the differences between COPD and healthy individuals in the levels of M1 macrophages, their secreted cytokines, and m6A regulators. The MAPK signaling pathway was significantly enriched in the list of differentially regulated genes between COPD and healthy individuals. We further analyzed the correlation between M1 macrophages, m6A, and the MAPK signaling pathway. Next, blood samples from COPD and healthy individuals were collected and analyzed by using flow cytometry, ELISA, and RT-PCR. Western blotting was performed using CSE-induced THP-1 cells. COPD and healthy mice were used for Me-RIP sequencing and flow cytometry experiments. Validation of the results of the above bioinformatics analysis by molecular biology experiments and sequencing techniques. Results We found that GEO data and blood specimens from COPD patients showed increased M1 macrophages, higher levels of IL-6 and TNF-α, and higher mRNA expression of key mediators of the MAPK signaling pathway (p38, ERK, and JNK). Western blotting showed increased expression of p38, ERK, and JNK in the CSE group. In contrast, the expression of m6A regulators was low. Also, M1 macrophages in COPD mice were hyperactivated and had reduced m6A modifications of p38, ERK, and JNK compared with control. Conclusion m6A may be involved in M1 macrophage hyperactivation by regulating the MAPK signaling pathway, thereby influencing the development of COPD.

show abstract

“…Macrophage classical activation is required in the normal protective immune response ( 58 ), particularly, in the early stage of the inflammatory reaction. However, chronic inflammatory diseases or excessive inflammation injury are not part of the normal protective response and immoderate macrophage polarization to M1 macrophages has been considered to be an important factor in chronic bronchitis or other inflammatory diseases ( 59 , 60 ). Mangiferin may inhibit macrophage classical activation via suppressing IRF5 expression levels.…”

Section: Discussionmentioning

confidence: 99%

Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression

Zhi-quan

Chen

et al. 2016

Molecular Medicine Reports

View full text Add to dashboard Cite

Mangiferin is a natural polyphenol and the predominant effective component of Mangifera indica Linn. leaves. For hundreds of years, Mangifera indica Linn. leaf has been used as an ingredient in numerous traditional Chinese medicine preparations for the treatment of bronchitis. However, the pharmacological mechanism of mangiferin in the treatment of bronchitis remains to be elucidated. Macrophage classical activation is important role in the process of bronchial airway inflammation, and interferon regulatory factor 5 (IRF5) has been identified as a key regulatory factor for macrophage classical activation. The present study used the THP-1 human monocyte cell line to investigate whether mangiferin inhibits macrophage classical activation via suppressing IRF5 expression in vitro. THP-1 cells were differentiated to macrophages by phorbol 12-myristate 13-acetate. Macrophages were polarized to M1 macrophages following stimulation with lipopolysaccharide (LPS)/interferon-γ (IFN-γ). Flow cytometric analysis was conducted to detect the M1 macrophages. Reverse transcription-quantitative polymerase chain reaction was used to investigate cellular IRF5 gene expression. Levels of proinflammatory cytokines and IRF5 were assessed following cell culture and cellular homogenization using enzyme-linked immunosorbent assay. IRF5 protein and nuclei co-localization was performed in macrophages with laser scanning confocal microscope immunofluorescence analysis. The results of the present study demonstrated that mangiferin significantly inhibits LPS/IFN-γ stimulation-induced classical activation of macrophages in vitro and markedly decreases proinflammatory cytokine release. In addition, cellular IRF5 expression was markedly downregulated. These results suggest that the inhibitory effect of mangiferin on classical activation of macrophages may be exerted via downregulation of cellular IRF5 expression levels.

show abstract

Involvement of mitogen-activated protein kinase activation in cyclooxygenase-2 and transforming growth factor-β production in alveolar macrophage from chronic bronchitis rats

Abstract: These results demonstrated COX-2, PGE(2) and TGF-β productions in AM of CB rats were significantly increased, which might be regulated by the different MAPK signaling pathway.

Cited by 6 publications

References 30 publications

An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills for pulmonary diseases

An integrated global chemomics and system biology approach to analyze the mechanisms of the traditional Chinese medicinal preparation Eriobotrya japonica – Fritillaria usuriensis dropping pills for pulmonary diseases

The Activation of M1 Macrophages is Associated with the JNK-m6A-p38 Axis in Chronic Obstructive Pulmonary Disease

Mangiferin inhibits macrophage classical activation via downregulating interferon regulatory factor 5 expression

Contact Info

Product

Resources

About