Involvement of Na⁺/Ca²⁺ exchanger in migration and contraction of rat cultured tendon fibroblasts

Abstract

In response to injury and inflammation of tendons, tendon fibroblasts are activated, migrate to the wound, and eventually induce contraction of the extracellular matrices to repair the tissue. .7 were expressed in cultured tendon fibroblasts. NCX2 mRNA was undetected. NCX3 expression was negligibly low. Immunofluorescence microscopy indicated that NCX1 protein localized in the plasma membrane especially at the microspikes of tendon fibroblasts. In the wound-healing scratch assay, the cells migrated toward the space created by a scratch and almost completely filled the space within 48 h. This phenomenon was significantly suppressed by KB-R7943 and SEA0400. Furthermore, the NCX inhibitors abrogated the tendon fibroblast-mediated collagen-matrix contractions. Two types of siRNAs for NCX1 also suppressed the migration and contraction of tendon fibroblasts. We conclude that NCX is expressed and mediates Ca 2+ influx in cultured tendon fibroblasts. Since the pharmacological inhibitors and siRNA for NCX1 suppressed motility and contractility of tendon fibroblasts, NCX may play an important role in the function of tendon fibroblasts in the wound healing.

Genetic Variation in the SLC8A1 Calcium Signaling Pathway Is Associated With Susceptibility to Kawasaki Disease and Coronary Artery Abnormalities

“…26 NCX1 is expressed on cells of mesenchymal origin, including fibroblasts, smooth muscle cells, and myofibroblasts, that all play key roles in aneurysm formation in KD patients. 10,13,[27][28][29] Transforming growth factor-β signaling leads to calcium flux in fibroblasts through NCX1 and results in increased expression of endothelial-mesenchymal transition (EMT)-related genes, including connective tissue growth factor and smooth muscle actin. 13 We have previously reported that myofibroblast-like cells expressing connective tissue growth factor in the wall of CAA and NCX1 may influence this process.…”

Inhibition of Rab1 GTPase and Endoplasmic Reticulum-to-Golgi Trafficking Underlies Statin's Toxicity in Rat Skeletal Myofibers

“…Cultured myofibers were separated from fibroblasts by the following way. To minimize the number of fibroblasts, which would otherwise eventually overgrow myofibers during culture (Sakamoto et al, 2007(Sakamoto et al, , 2009, the preparation was allowed to settle for 10 min. The sediments were transferred to a 60-mm dish and cultured overnight.…”

Sodium-Calcium Exchanger 1 Regulates Epithelial Cell Migration via Calcium-dependent Extracellular Signal-regulated Kinase Signaling

“…NCX1 has been reported to promote the migration of a diverse group of non-epithelial cells such as endothelial cells, oligodendrocytes, myofibroblasts, microglia, and pancreatic cancer cells (53)(54)(55)(56)(57)(58)(59)(60). Inhibition or knockdown of NCX1 reduced intracellular calcium and prevented migration.…”