Involvement of norepinephrine in the hyperglycemic responses of the freshwater giant prawn, Macrobrachium rosenbergii, under cold shock

Hsieh, Shu‐Ling; Chen, S.M.; Yang, Yueying; Kuo, Ching-Ming

doi:10.1016/j.cbpa.2005.12.009

Cited by 42 publications

(30 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The CAs can be metabolized by monoamine oxidase (MAO) in the cytoplasm for degradation. NE contents in haemolymph of M. rosenbergii that were transferred from 28 C to 23, 20, 18 and 15 C for 30 min showed increasing trends of changes with reduced temperature [6]. In the present study, an injection of NE also increased the haemolymph NE level from 30 to 120 min indicating that the haemolymph NE of prawns injected with NE at 50 pmol/prawn can keep a higher level than physiology one at least 2 h.…”

Section: Discussionsupporting

confidence: 46%

“…Phentolamine (Phe), prazosin (Pra), propranolol (Pro), and metoprolol (Met) are considered adrenoceptor (AR) antagonists that interfere with the physiological actions of NE by combining with and blocking its receptor [6]. NE and 4 AR antagonists (Phe, an a1þ2 AR antagonist, P-7547; Pra, an a1 AR antagonist, P-7791; Pro, a b1þ2 AR antagonist, P-0884; and Met, a b1 AR antagonist, M-5391, Sigma) were individually dissolved in cacodylate buffer to be an NE concentration at 10 À5 mol/L and an AR antagonist concentration at 10 À4 mol/L as a stock solution.…”

Section: Modulation Of Immune Parameters and Genes Expressions Of Haementioning

confidence: 99%

“…M. rosenbergii exposed to stressful conditions such as hypoxia, cold shock, high levels of ammonia and pH values exhibited immunosuppression and had increased susceptibility to pathogen infection [5]. A positive and significant correlation is observed between NE content and glucose level in the haemolymph of M. rosenbergii under acute cold shock, indicating that NE plays an important role in the hyperglycemic responses of prawn under cold shock [6]. The inhibited immune responses and resistance against bacterial infection were observed in both Litopenaeus vannamei [7] and M. rosenbergii [8] received NE by injection.…”

Section: Introductionmentioning

confidence: 98%

“…In most vertebrates, the influence of NE is restricted to a-and b1-ARs [11]. Hsieh et al [6] indicated that the action of NE primarily regulated hyperglycemic responses of M. rosenbergii through both a1-and b1-ARs. Chang et al [8] showed that NE suppressed the immune system, and promoted the susceptibility of M. rosenbergii to Lactococcus garvieae via both a1-and b1-ARs.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The acute modulation of norepinephrine on immune responses and genes expressions via adrenergic receptors in the giant freshwater prawn, Macrobrachium rosenbergii

Chang

Tsai

Jiang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 46%

Section: Modulation Of Immune Parameters and Genes Expressions Of Haementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The acute modulation of norepinephrine on immune responses and genes expressions via adrenergic receptors in the giant freshwater prawn, Macrobrachium rosenbergii

Chang

Tsai

Jiang

et al. 2015

Fish & Shellfish Immunology

View full text Add to dashboard Cite

“…In the hepatopancreas of the euryhaline pacific white shrimp Litopenaeus vannamei, the responses of digestive enzymes activity to extreme environmental salinity has been suggested to be related to the possibility of deriving extra energy upon osmoregulation (Li et al 2008). Dopamine (DA) is an important neurotransmisor and neurohormone in crustaceans (Fingerman et al 1994, Clark et al 2008, Christie 2011 and has been shown to participate in the regulation of metabolic responses to environmental stresses (Lorenzon, 2005, Hsieh et al 2006. In euryhaline crabs, DA is involved in the regulation of osmoregulation process by modulating osmolyte concentration in the hemolymph and branchial transport of Na + and Na + / K + ATPase activity (Mo et al 1998, Schleich et al 1999, Morris 2001, Halperin et al 2004, Genovese et al 2006.…”

Section: Introductionmentioning

confidence: 99%

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

Michiels¹,

Valle²,

Mañanes³

2012

Sci. Mar.

View full text Add to dashboard Cite

SUMMARY:We studied the occurrence and characteristics of lipase activity and the response of lipase and proteolytic activity to salinity and dopamine injections in hepatopancreas of the euryhaline crab (Cyrtograpsus angulatus). Lipase activity was maximal at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent K m =0.019 mM), was higher at 37°C and appeared to be cold tolerant, being also high at 4°C. In 10 psu (hyper-regulation conditions), lipase and proteolytic activity were about 3 and 5 times higher, respectively, than in 35 psu (osmoconformation). In 40 psu (hypo-regulation), lipase activity was about three times higher than in 35 psu, while proteolytic activity was similar. Lipase activity was inhibited in vivo by 10 -4 M dopamine in 35 psu but not in 10 or 40 psu. Proteolytic activity was not affected by 10 -4 M dopamine. The differential responses of lipase and proteolytic activity to salinity and dopamine suggest the occurrence of distinct digestive adjustments and mechanisms of regulation upon osmoregulatory conditions. This study contributes to a better understanding of the complexity of the biochemical adaptations to salinity in euryhaline crabs. The fact that higher digestive enzyme activities could be associated with a differential digestive capacity potentially leading to enhanced availability of energy substrates is discussed.Keywords: Cyrtograpsus angulatus, euryhaline crabs, salinity, hepatopancreas, digestive enzymes, dopamine, Mar Chiquita lagoon. RESUMEN: EfEcto dE la salinidad ambiEntal y dE la inyEcción dE dopamina sobrE Enzimas digEstivas clavE En hEpatopáncrEas dEl cangrEjo EurihalinoCyrtograpsus angulatus (dEcapoda: brachyura: varunidaE). -Se estudió la existencia y características bioquímicas de actividad de lipasa y la respuesta a salinidad y a dopamina de actividad de lipasa y proteolítica total en el hepatopáncreas del cangrejo eurihalino Cyrtograpsus angulatus. La actividad de lipasa fue máxima a pH 8,5; mostró una cinética michaeliana (K m =0.019 mM); fue mayor a 37°C, y resistente a baja temperatura (4°C). En 10 psu (condición de hiperregulación), las actividades de lipasa y proteolítica fueron 3 y 5 veces mayores, respectivamente, que en 35 psu (condición de osmoconformación). En 40 psu (condición de hiporregulación), la actividad de lipasa fue de alrededor 3 veces mayor que en individuos aclimatados a 35 psu, mientras que la actividad proteolítica fue similar. La actividad de lipasa fue inhibida in vivo por dopamina 10 -4 M en 35 psu pero no fue afectada en 10 o 40 psu. Dopamina 10 -4 M no afectó la actividad proteolítica. Las respuestas de las actividades de lipasa y proteolítica a salinidad y a dopamina permiten sugerir la existencia de ajustes digestivos y mecanismos de regulación diferenciales en relación al estado osmorregulatorio. Los resultados constituyen un aporte relevante al conocimiento sobre las complejas adaptaciones a nivel bioquímico a la salinidad en cangrejos eurihalinos.

show abstract

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

et al. 2023

View full text Add to dashboard Cite

Ocypode quadrata, a Ghost crab species found along the western Atlantic coast, is considered a bioindicator of anthropogenic impact on sandy beaches. Ghost Crabbing, a touristic activity in which ghost crabs are chased just for fun, is a potentially threatening activity for this crab. In crustaceans, metabolites such as glucose and lactate, and the gene expression of crustacean hyperglycemic hormone (CHH) and heat shock proteins (HSPs) increase when the animals are exposed to several types of stress, including alterations in temperature, salinity, or exposure to xenobiotics. This work was developed to identify if being chased by humans would affect these markers of stress in this species of crab. The effects of chasing stress on hemolymph and tissue metabolites and the gene expression levels of CHH and HSP70 were investigated. The levels of lactate in the hemolymph of stressed crabs were six times higher than those of control crabs immediately after chasing and decreased progressively during recovery, indicating an active anaerobic metabolism during the stress. On the contrary, glucose levels in the hemolymph of the stressed crabs increased progressively from 30 to 60 min after chasing, indicating an inverse correlation between glucose and lactate and the conversion of lactate to glucose by gluconeogenesis. In stressed crabs, the levels of triglycerides in the hemolymph decreased 30 min after chasing, while the opposite tended to occur in the hepatopancreas, indicating that during recovery, the crabs use triglycerides as energy source to sustain aerobic metabolism. Finally, this study demonstrates that ghost crabs are stressed by minimum human contact and that “ghost crabbing” must not be encouraged as a tourist activity.

show abstract

Involvement of norepinephrine in the hyperglycemic responses of the freshwater giant prawn, Macrobrachium rosenbergii, under cold shock

Cited by 42 publications

References 27 publications

The acute modulation of norepinephrine on immune responses and genes expressions via adrenergic receptors in the giant freshwater prawn, Macrobrachium rosenbergii

The acute modulation of norepinephrine on immune responses and genes expressions via adrenergic receptors in the giant freshwater prawn, Macrobrachium rosenbergii

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

The impact of chasing stress on the metabolism of the Atlantic Ghost Crab Ocypode quadrata (Fabricius, 1787)

Contact Info

Product

Resources

About