Involvement of opioidergic and serotonergic systems in the analgesic activity of <i>Cissus quadrangularis</i> L. stem extract in mice

Nie, Tan Wee; Shukkoor, Mohamed Saleem Abdul; Nair, Rajesh Sreedharan; Amiruddin, Fatimah Khursiah Binti; Ramasamy, Shamala

doi:10.1515/jbcpp-2014-0035

Cited by 6 publications

(3 citation statements)

References 31 publications

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“…Cissus quadrangularis treatment improved metabolic parameters in rats on a high-fat diet. Studies have explored the pharmacological properties of Cissus quadrangularis , including anti-inflammatory [ 14 , 15 ] analgesic [ 15 , 16 ], antimicrobial [ 17 ], and antioxidant effects [ 18 ]. The plant’s medicinal properties are linked to bioactive compounds such as resveratrol, piceatannol, pallidol, parthenocissine A, stilbene derivatives, and quadrangularins A, B, and C [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

et al. 2023

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Cissus quadrangularis is a nutrient-rich plant with a history of use in traditional medicine. It boasts a diverse range of polyphenols, including quercetin, resveratrol, β-sitosterol, myricetin, and other compounds. We developed and validated a sensitive LC-MS/MS method to quantify quercetin and t-res biomarkers in rat serum and applied this method to pharmacokinetic and stability studies. The mass spectrometer was set to negative ionization mode for the quantification of quercetin and t-res. Phenomenex Luna (C18(2), 100 A, 75 × 4.6 mm, 3 µ) column was utilized to separate the analytes using an isocratic mobile phase consisting of methanol and 0.1% formic acid in water (82:18). Validation of the method was performed using various parameters, including linearity, specificity, accuracy, stability, intra-day, inter-day precision, and the matrix effect. There was no observed significant endogenous interference from the blank serum. The analysis was completed within 5.0 min for each run, and the lower limit of quantification was 5 ng/mL. The calibration curves showed a linear range with a high correlation coefficient (r2 > 0.99). The precision for intra- and inter-day assays showed relative standard deviations from 3.32% to 8.86% and 4.35% to 9.61%, respectively. The analytes in rat serum were stable during bench-top, freeze-thaw, and autosampler (−4 °C) stability studies. After oral administration, the analytes showed rapid absorption but underwent metabolism in rat liver microsomes despite being stable in simulated gastric and intestinal fluids. Intragastric administration resulted in higher absorption of quercetin and t-res, with greater Cmax, shorter half-life, and improved elimination. No prior research has been conducted on the oral pharmacokinetics and stability of anti-diabetic compounds in the Ethanolic extract of Cissus quadrangularis EECQ, making this the first report. Our findings can provide the knowledge of EECQ’s bioanalysis and pharmacokinetic properties which is useful for future clinical trials.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

et al. 2023

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“…Veldt Grape is a vining plant native to India and Africa that has been used medicinally for centuries in these regions by traditional practitioners. A lot of research has been conducted globally on the efficacy of Cissus quadrangularis including, its anti-inflammatory effects [11][12] , anti-osteoporotic effects 13 , Antioxidant activity 14 , and effects on skeletal systems 15 . Pandit et al (2020) reported the antifungal effects of Cissus quadrangularis extract on onchomycosis rather than fluconazole 16 .…”

Section: Introductionmentioning

confidence: 99%

Effect of Cissus quadrangularis on Fracture Healing in Laboratory Animal

Zahan

Hasan²,

Mallik³

et al. 2022

J Health Biol Sci.

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Objetivos: avaliar a eficácia da pasta de Cissus quadrangularis na consolidação de fraturas em coelhos fraturados artificialmente. Metodos: quinze coelhos foram separados em três grupos (A, B e C). A pasta foi aplicada nos grupos B e C (Grupo de Tratamento) pelos métodos de redução fechada e redução aberta de gerenciamento de fraturas; respectivamente. O grupo A foi mantido como controle. O parâmetro sanguíneo e as propriedades de cicatrização de fraturas em todos os animais foram monitorados e examinados, rotineiramente, durante o período do estudo. Resultados: ambos os grupos tratados revelaram níveis séricos de cálcio (SCL) mais baixos do que o grupo controle, após 24 horas de fratura que se tornou normal no 14º dia. A cicatrização da fratura nos grupos tratados foi iniciada mais rapidamente do que o grupo controle, com ponte completa de descontinuidade por um calo ósseo distinto na linha de fratura no dia 7 e apagamento completo da linha de fratura no dia 14. Conclusao: não encontramos nenhum tipo de anomalia, desvios clínicos e alteração do nível sérico de cálcio no 14º dia da fratura nos animais tratados, portanto, a pasta Veldt Grape pode ser aplicável ao manejo da fratura em animais.

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“…In Africa, this plant is used against gout, venereal diseases, leucorrhoea, syphilis, haemorrhoids, diarrhoea, dysentery and as an aphrodisiac (Shirwaikar et al, 2003). Work carried out on C. quadrangularis has shown its analgesic (Dora et al 2017), antiin ammatory (Banu et al 2012;Nie et al 2015), anti-osteoporotic (Potu et al 2009) and antioxidant (Lekshmi et al 2015) properties, its effects on the skeletal system (Bhagat et al 2009), antibacterial, antifungal, deworming, antihemorrhoidal and analgesic (Mishra et al 2010)…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory, anti-infammatory and antioxidant activities of aqueous and ethanolic extracts of Cissus quadrangularis Linn. (Vitaceae) in chronic pain

Feigni

Mbiantcha

Yousseu

et al. 2022

Preprint

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Cissus quadrangularis Linn. (Vitaceae) is a plant used to treat injured tendons, broken bones, asthma, stomach ache, scurvy and digestive disorders. The present study purposed to evaluate the antihyperalgesic effects (in vivo) and the immunomodulatory, antioxidant and anti-inflammatory properties (in vitro) of aqueous and ethanolic extracts of Cissus quadrangularis (C. quadrangularis). Immunomodulatory (chemiluminescence, cytokines and cell proliferation), anti-inflammatory (protein denaturation, 5-lipoxygenase, cyclooxygenase 1 and 2) and antioxidant (DPPH, ABTS and NO) tests were performed in vitro, while the anti- hyperalgesic (vincristine) investigations were conducted in vivo on Wistar rats. The results revealed that extracts developed immunomodulatory activity by inhibiting the production of ROS (intracellular/extracellular), of TNFα, IL-1β, IL-6 as well as inhibiting cell proliferation, and by stimulating the production of IL-10. The anti-inflammatory activity of the extracts was demonstrated by an inhibition of 5-LOX, protein denaturation and cyclooxygenases 1 and 2. In addition, extracts showed interesting scavenging effects, attesting their antioxidant potential. The extracts administered to the animals (180 and 360 mg/kg) inhibited (p < 0.001) hyperalgesia and allodynia in animals. These extracts also led to the reduction in serum and sciatic nerve levels of TNFα, IL-1β and IL-6, as well as to an increase in cell growth factors (NGF and IGF) production of treated animals. These results suggest that extracts of C. quadrangularis use immunomodulatory, anti-infammatory and antioxidant capacity to prevent and/cure painful neuropathy after vincristine administration. C. quadrangularis is therefore a promising natural substance for the management of neuropathic pain.

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Involvement of opioidergic and serotonergic systems in the analgesic activity of Cissus quadrangularis L. stem extract in mice

Abstract: The chloroform extract was found to be more potent than methanol extract in inducing analgesic effect in mice, and the analgesic activity may be mediated through opioidergic and serotonergic pathways.

Cited by 6 publications

References 31 publications

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

Simultaneous Estimation of Quercetin and trans-Resveratrol in Cissus quadrangularis Extract in Rat Serum Using Validated LC-MS/MS Method: Application to Pharmacokinetic and Stability Studies

Effect of Cissus quadrangularis on Fracture Healing in Laboratory Animal

Immunomodulatory, anti-infammatory and antioxidant activities of aqueous and ethanolic extracts of Cissus quadrangularis Linn. (Vitaceae) in chronic pain

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