Involvement of periodic deacylation-acylation cycles of mitochondrial phospholipids during Sr2+-induced oscillatory ion transport in rat liver mitochondria

Wiswedel, Ingrid; Barnstorf, Ulrike; Augustin, Wolfgang; Holmuhamedov, Ekhson; Medvedev, B. I.; Evtodienko, Yu. V.

doi:10.1016/0005-2736(82)90371-6

Cited by 15 publications

(4 citation statements)

References 32 publications

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“…458 With the exception of the lysosomal soluble enzyme that has an acidic pH optimum and is inhibited by Ca 2+ , all other membrane-associated PLA 2 require an alkaline pH and Ca 2+ for optimal activity. PLA 2 has been implicated in phospholipid deacylation and reacylation cycles 459 and thereby in the regulation of steady state levels of lysophospholipids and free fatty acid which in turn may influence permeability properties, Ca 2+ -release, and the efficiency of energy-linked processes. 460 The Ca 2+ -induced translocation of PLA 2 in Kupffer cells between the cytosol and the membrane fraction is considered a regulatory mechanism for PLA 2 by bringing the enzyme to its substrate.…”

Section: Pla 2 In Liver Disordersmentioning

confidence: 99%

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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Phospholipase A2 (PLA2) is an enzyme that catalyzes the hydrolysis of membrane phospholipids. This article reviews the source and structure of PLA2, the involvement of the enzyme in various biological and pathological phenomena, and the usefulness of PLA2 assays in laboratory diagnostics. Of particular importance is the role of PLA2 in the cellular production of mediators of inflammatory response to various stimuli. Assays for PLA2 activity and mass concentration are discussed, and the results of enzyme determinations in plasma from patients with different pathological conditions are presented. The determination of activity and mass concentration in plasma is particularly useful in the diagnosis and prognosis of pancreatitis, multiple organ failure, septic shock, and rheumatoid arthritis. A very important result is the demonstration that PLA2 is an acute phase protein, like CRP. Indeed, there is a close correlation between PLA2 mass concentration and CRP levels in several pathological conditions. Although the determination of C-reactive protein is much easier to perform and is routinely carried out in most clinical laboratories, the assessment of PLA2 activity or mass concentration has to be considered as a reliable approach to obtain a deeper understanding of some pathological conditions and may offer additional information concerning the prognosis of several disorders.

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Section: Pla 2 In Liver Disordersmentioning

confidence: 99%

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Kaiser

1999

Critical Reviews in Clinical Laboratory Sciences

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“…Periodic influx and efflux of Ca 2+ during Ca 2+ -induced oscillations in mitochondria may provide hydrolysis of oxidized phospholipids by Ca 2+ -dependent PLA 2 in the phase of Ca 2+ accumulation in the matrix, followed by a decrease in the activity of phospholipase A 2 after the ion release. This suggestion is supported by the data on periodic changes in the content of lysophospholipids in mitochondria during Sr 2+ -dependent oscillations [60]. Therefore, the oscillatory mode of mitochondrial function may contribute to the protection of cells and mitochondria from oxidative stress and calcium overload, which is associated with the development of many pathologies, such as cardiovascular diseases, neurological disorders, diabetes, obesity, cancer, and others [4,5,16,61].…”

Section: Discussionmentioning

confidence: 72%

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

et al. 2022

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Mitochondria are capable of synchronized oscillations in many variables, but the underlying mechanisms are still unclear. In this study, we demonstrated that rat liver mitochondria, when exposed to a pulse of Sr2+ ions in the presence of valinomycin (a potassium ionophore) and cyclosporin A (a specific inhibitor of the permeability transition pore complex) under hypotonia, showed prolonged oscillations in K+ and Sr2+ fluxes, membrane potential, pH, matrix volume, rates of oxygen consumption and H2O2 formation. The dynamic changes in the rate of H2O2 production were in a reciprocal relationship with the respiration rate and in a direct relationship with the mitochondrial membrane potential and other indicators studied. The pre-incubation of mitochondria with Ca2+(Sr2+)-dependent phospholipase A2 inhibitors considerably suppressed the accumulation of free fatty acids, including palmitic and stearic acids, and all spontaneous Sr2+-induced cyclic changes. These data suggest that the mechanism of ion efflux from mitochondria is related to the opening of short-living pores, which can be caused by the formation of complexes between Sr2+(Ca2+) and endogenous long-chain saturated fatty acids (mainly, palmitic acid) that accumulate due to the activation of phospholipase A2 by the ions. A possible role for transient palmitate/Ca2+(Sr2+)-induced pores in the maintenance of ion homeostasis and the prevention of calcium overload in mitochondria under pathophysiological conditions is discussed.

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“…The continual turnover of lipids via a reacylation-deacylation metabolic cycle provides a point of control for lysolipid concentration within membranes. This metabolic cycle may be temporally correlated with alterations in membrane permeability both in mitochondria (Wiswedel et al, 1982) and brain synaptosomes (Iwata et al, 1986), suggesting that membrane permeability may be controlled by utilizing lysolipids to affect membrane barrier properties.…”

Section: Introductionmentioning

confidence: 99%

Polymorphic phase behavior of lysophosphatidylethanolamine dispersions. A thermodynamic and spectroscopic characterization

Slater

Huang

Adams

et al. 1989

Biophysical Journal

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We have investigated the phase behavior of aqueous dispersions of a series of synthetic lysophosphatidylethanolamines as a function of the acyl chain length. Lysophosphatidylethanolamines exhibit phase polymorphism encompassing a well-ordered crystalline phase which may arise either from a metastable interdigitated lamellar gel phase or a metastable micellar phase. The time course of interconversion between these various phases have been outlined by observing the low temperature incubation time dependence of the calorimetric thermograms. We have determined differences in structure of these phases by Raman spectroscopy and 31P nuclear magnetic resonance spectroscopy. It appears that a principal contribution to this polymorphic phase behavior lies in the nature of headgroup hydration and headgroup-headgroup interactions.

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Involvement of periodic deacylation-acylation cycles of mitochondrial phospholipids during Sr2+-induced oscillatory ion transport in rat liver mitochondria

Cited by 15 publications

References 32 publications

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

Polymorphic phase behavior of lysophosphatidylethanolamine dispersions. A thermodynamic and spectroscopic characterization

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Involvement of periodic deacylation-acylation cycles of mitochondrial phospholipids during Sr2+-induced oscillatory ion transport in rat liver mitochondria

Cited by 15 publications

References 32 publications

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

Phospholipase A2: Its Usefulness in Laboratory Diagnostics

The Short-Term Opening of Cyclosporin A-Independent Palmitate/Sr2+-Induced Pore Can Underlie Ion Efflux in the Oscillatory Mode of Functioning of Rat Liver Mitochondria

Polymorphic phase behavior of lysophosphatidylethanolamine dispersions. A thermodynamic and spectroscopic characterization

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Phospholipase A₂: Its Usefulness in Laboratory Diagnostics

Phospholipase A₂: Its Usefulness in Laboratory Diagnostics