Involvement of proteases during nodule senescence in leguminous plants

Li, Yang; Syska, Camille; Garcia, Isabelle; Frendo, Pierre; Boncompagni, Éric

doi:10.1002/9781119409144.ch85

Cited by 2 publications

(4 citation statements)

References 92 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cysteine proteases (CYPs) are well‐known markers of nodule senescence (Fujie et al ., 2009; Yang et al ., 2019; Zhou et al ., 2021). We observed that a set of CYPs were upregulated in NAC‐OE nodules and clustered into the Nitrate‐C2 dataset, suggesting a role of NAC094 in the transcriptional regulation of multiple CYPs , including CYP1 (Lj3g3v1642060), CYP2 (Lj1g3v4047270), CYP3 (Lj0g3v0000069), and CYP4 (Lj1g3v4047250) (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Leaf and nodule senescence are two comparable biological processes that share responsive genes encoding enzymes involved in the degradation of organelles and macromolecules as well as in nutrient remobilization (Van de Velde et al ., 2006; De Michele et al ., 2009). The degradation of chloroplast, chlorophyll, and chlorophyll‐binding proteins in senescent leaves functionally corresponds to the degradation of symbiosome, heme, and heme‐binding proteins (mostly Lbs) in senescent nodules, for example, multiple protease genes were actively expressed in senescent leaves and nodules (Yang et al ., 2019; Guo et al ., 2021; Zhou et al ., 2021). The mechanism of leaf senescence has been intensively studied, in sharp contrast to the poorly investigated nodule senescence.…”

Section: Discussionmentioning

confidence: 99%

“…S8). These genes encode cysteine proteases with a purported function in the degradation of Lbs and other nodule proteins (Fujie et al ., 2009; Yang et al ., 2019). Cysteine proteases with acidic pH optima (5.0–5.5) are especially active in degrading Lbs and the intracellular pH becomes acidic in senescing nodules (Pladys et al ., 1988; Pladys & Vance, 1993).…”

Section: Discussionmentioning

confidence: 99%

“…S10). These results indicate that multiple Lb degradation pathways, such as autophagy and other proteolytic phenomena, might be present in different types of nodule senescence (Vauclare et al ., 2010; Yang et al ., 2019). Notably, there is an apparent inconsistency between the absence of Lb transcripts and the presence of Lb proteins in nodules of nac094 mutants (Fig.…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

A transcription factor of the NAC family regulates nitrate‐induced legume nodule senescence

et al. 2023

View full text Add to dashboard Cite

Summary Legumes establish symbioses with rhizobia by forming nitrogen‐fixing nodules. Nitrate is a major environmental factor that affects symbiotic functioning. However, the molecular mechanism of nitrate‐induced nodule senescence is poorly understood. Comparative transcriptomic analysis reveals an NAC‐type transcription factor in Lotus japonicus, LjNAC094, that acts as a positive regulator in nitrate‐induced nodule senescence. Stable overexpression and mutant lines of NAC094 were constructed and used for phenotypic characterization. DNA‐affinity purification sequencing was performed to identify NAC094 targeting genes and results were confirmed by electrophoretic mobility shift and transactivation assays. Overexpression of NAC094 induces premature nodule senescence. Knocking out NAC094 partially relieves nitrate‐induced degradation of leghemoglobins and abolishes nodule expression of senescence‐associated genes (SAGs) that contain a conserved binding motif for NAC094. Nitrate‐triggered metabolic changes in wild‐type nodules are largely affected in nac094 mutant nodules. Induction of NAC094 and its targeting SAGs was almost blocked in the nitrate‐insensitive nlp1, nlp4, and nlp1 nlp4 mutants. We conclude that NAC094 functions downstream of NLP1 and NLP4 by regulating nitrate‐induced expression of SAGs. Our study fills in a key gap between nitrate and the execution of nodule senescence, and provides a potential strategy to improve nitrogen fixation and stress tolerance of legumes.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

A transcription factor of the NAC family regulates nitrate‐induced legume nodule senescence

et al. 2023

View full text Add to dashboard Cite

show abstract

A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules

Shimoda,

Yamaya-Ito,

Hakoyama

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Symbiotic nitrogen fixation is a highly coordinated process involving legume plants and nitrogen-fixing bacteria known as rhizobia. In this study, we investigated a novel Fix − mutant of the model legume Lotus japonicus that develops root nodules with endosymbiotic rhizobia but fails in nitrogen fixation. Map-based cloning identified the causal gene encoding the filamentation temperature-sensitive H (FtsH) protein, designated as LjFtsH4 . The LjFtsH4 gene was expressed in all plant organs without increased levels during nodulation. Subcellular localization revealed that LjFtsH4, fused with a fluorescent protein, localized in mitochondria. The Ljftsh4 mutant nodules showed signs of premature senescence, including symbiosome membrane collapse and bacteroid disintegration. Additionally, nodule cells of Ljftsh4 mutant displayed mitochondria with indistinct crista structures. Grafting and complementation tests confirmed that the Fix − phenotype was determined by the root genotype, and that protease activity of LjFtsH4 was essential for nodule nitrogen fixation. Furthermore, the ATP content in Ljftsh4 mutant roots and nodules was lower than in the wild-type, suggesting reduced mitochondrial function. These findings underscore the critical role of LjFtsH4 in effective symbiotic nitrogen fixation in root nodules. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-78295-5.

show abstract

Involvement of proteases during nodule senescence in leguminous plants

Cited by 2 publications

References 92 publications

A transcription factor of the NAC family regulates nitrate‐induced legume nodule senescence

A transcription factor of the NAC family regulates nitrate‐induced legume nodule senescence

A mitochondrial metalloprotease FtsH4 is required for symbiotic nitrogen fixation in Lotus japonicus nodules

Contact Info

Product

Resources

About