Involvement of reactive oxygen species on gentamicin-induced mesangial cell activation

Martı́nez-Salgado, Carlos; Eleno, Nélida; Tavares, Paula; Rodríguez-Barbero, Alicia; García-Criado, Javier; Bolaños, Juan P.; López‐Novoa, José M.

doi:10.1046/j.1523-1755.2002.00635.x

Cited by 70 publications

(53 citation statements)

References 36 publications

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“…These findings correlated well with the renal histological examination, which revealed renal tubular necrosis and interstitial nephritis. Previous studies found that GEN is capable of enhancing the formation of ROS, including O 2 −• [6] and H 2 O 2 [7][8][9] radicals that stimulate [45] the mesangial cells contraction, and decreasing the GFR without alternating the glomerular structure. It was also observed that increased O 2 −• could react with nitric oxide (NO, a vasodilator) to form peroxynitrite, a cytotoxic oxidant radical specie, which decreases the GFR.…”

Section: Discussionmentioning

confidence: 99%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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In the present study, we investigated the protective effect of Withania somnifera, an indigenous medicinal herb used in ayurvedic traditional systems for more than 3000 years in India, on gentamicin (GEN)-induced nephrotoxicity. The root extract of three different doses of W. somnifera (viz., 250, 500, and 750 mg/kg) was administered orally to rats for 14 days before GEN treatment and thereafter concurrently with GEN (100 mg/kg) for 8 days. Nephrotoxicity was evident in GEN-treated rats by significant increase in kidney weight, urea, creatinine, urinary protein, and glucose, and significant reduction in body weights and potassium, which was histopathologically confirmed by tubular necrosis. In contrast W. somnifera (500 mg/kg) significantly reversed these changes as evidenced microscopically when compared to other two doses of W. somnifera (250 and 750 mg/kg), and there were no significant changes in the levels of sodium in the experimental animals compared to control. Thus, our results suggested the nephroprotective effect of Withania somnifera, which could be by enhancing antioxidant activity with natural antioxidants and scavenging the free radicals.

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Section: Discussionmentioning

confidence: 99%

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Jeyanthi

Subramanian

2009

Renal Failure

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“…MCs also resort to proliferation upon activation by proinflammatory mediator-induced oxidative stress. This is accompanied by the de novo expression of ␣-smooth muscle actin (␣-SMA) and TGF-␤ secretion (13,24,32,34,48). In parallel to studies regarding the role of MCs in chronic kidney immunotoxicity, there is evidence of immunosuppressive roles of a class of T cells including invariant and CD1D-dependent natural killer (NK)T cells (17).…”

mentioning

confidence: 95%

“…2C). Oxidative stress and other inflammatory stimuli of a primarily systemic nature have been shown to increase TNF-␣ levels and TNF-␣ release from proximal tubular cells, podocytes, and MCs (13,24,28,34). The results showed that levels of TNF-␣ were significantly H: morphometric analysis of IFN-␥ immunoreactivity in the LC, LC ϩ BDCM, NAFLD, NAFLD ϩ BDCM, and NAFLD ϩ BDCM ϩ DAS groups.…”

Section: Nafld Potentiates Metabolic Oxidative Stress Via Cyp2e1mentioning

confidence: 99%

NKT cell modulates NAFLD potentiation of metabolic oxidative stress-induced mesangial cell activation and proximal tubular toxicity

Alhasson

Dattaroy

Das

et al. 2016

American Journal of Physiology-Renal Physiology

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“…4 There is evidence that reactive oxygen species (ROS) are involved in renal damage. 5 Consequently, administration of several compounds with antioxidant activity may have the capacity to protect the kidney against the deleterious effects of drugs known to enhance the generation of ROS like GM. 6 Green tea (GT) is widely consumed in the eastern world.…”

Section: Introductionmentioning

confidence: 99%

Amelioration of gentamicin nephrotoxicity by green tea extract in uninephrectomized rats as a model of progressive renal failure

Salem

Kamel

et al. 2010

Renal Failure

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Rationale: Gentamicin (GM) is an effective antibiotic against severe infection but has limitations related to nephrotoxicity. This study investigates whether green tea extract (GTE), an antioxidant, could ameliorate the nephrotoxic effect of GM in uninephrectomized rats. Objectives: The right kidneys of 40 rats were surgically removed and 1 week later the animals were divided into four groups (n = 10). Group 1 served as control, Group 2 as GTE group, Group 3 as GM group, and Group 4 as GM+GTE group. Kidney function, inflammatory cytokine TNF-a, oxidant and antioxidant parameters of renal tissue, as well as histopathological studies were assessed. Main findings: Injecting uninephrectomized rats with GM induced renal dysfunction as shown by significant elevations in serum creatinine and urea. Serum TNF-a and oxidative stress parameters (superoxide anion and lipid peroxides) were also significantly increased. On the contrary, antioxidative parameters [superoxide dismutase (SOD), catalase (CAT), and reduced glutathione (GSH)] were significantly decreased. Histopathological examination of renal tissue illustrated features of degeneration, marked cellular infiltration, tubular dilatation, and varying degrees of necrosis. GTE given to GM rats reduced these nephrotoxicity parameters. Serum creatinine, urea, and TNF-a were almost normalized in the GM+GTE group. The oxidative stress parameters were significantly decreased and the antioxidative parameters were significantly increased. Conclusion: GTE ameliorates GM-induced nephrotoxicity and oxidative damage by improving antioxidant defense and tissue integrity. Further human studies are necessary to demonstrate the antioxidant effects of GTE on renal diseases. Nevertheless, green tea (GT) may offer an inexpensive, nontoxic, and effective intervention strategy in subjects with a risk for GM-induced nephrotoxicity.

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Involvement of reactive oxygen species on gentamicin-induced mesangial cell activation

Cited by 70 publications

References 36 publications

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

Nephroprotective Effect ofWithania somnifera:A Dose-Dependent Study

NKT cell modulates NAFLD potentiation of metabolic oxidative stress-induced mesangial cell activation and proximal tubular toxicity

Amelioration of gentamicin nephrotoxicity by green tea extract in uninephrectomized rats as a model of progressive renal failure

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