Involvement of RNA chaperone hfq in the regulation of antibiotic resistance and virulence in Shigella sonnei

Wang, Ya; Teng, Yanli; Geng, Jiao; Long, Jinzhao; Yang, Haiyan; Duan, Guangcai; Chen, Shuaiyin

doi:10.1016/j.resmic.2023.104047

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…The incomplete complementary pairing of most ncRNAs with the target mRNA sequence can lead to two results: (1) Blocking the ribosome binding sites and suppressing translation; (2) Secondary structure melting, exposing the nucleose binding site and translation start site, leading to translation activation ( Vogel and Sharma, 2005 ; Fröhlich and Vogel, 2009 ). Moreover, since the instabilized base pairing between the ncRNAs and their target mRNAs, the RNA chaperone protein Hfq, binding protein Fino/ProQ family, CsrA/RsmA family and other regulators usually facilitate imperfect base pairing between ncRNAs and mRNAs, leading to regulate the translation initiation frequency or the stability of target mRNAs ( Liao and Smirnov, 2023 ; Wang et al., 2023 ; Yu and Zhao, 2023 ). In this chapter, we will explore some research on ncRNAs that regulate the mechanisms of AMR from two perspectives.…”

Section: Bacterial Epigenetics Mediating Antibiotic Resistancementioning

confidence: 99%

Antimicrobial resistance and mechanisms of epigenetic regulation

Wang

2023

Front. Cell. Infect. Microbiol.

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The rampant use of antibiotics in animal husbandry, farming and clinical disease treatment has led to a significant issue with pathogen resistance worldwide over the past decades. The classical mechanisms of resistance typically investigate antimicrobial resistance resulting from natural resistance, mutation, gene transfer and other processes. However, the emergence and development of bacterial resistance cannot be fully explained from a genetic and biochemical standpoint. Evolution necessitates phenotypic variation, selection, and inheritance. There are indications that epigenetic modifications also play a role in antimicrobial resistance. This review will specifically focus on the effects of DNA modification, histone modification, rRNA methylation and the regulation of non-coding RNAs expression on antimicrobial resistance. In particular, we highlight critical work that how DNA methyltransferases and non-coding RNAs act as transcriptional regulators that allow bacteria to rapidly adapt to environmental changes and control their gene expressions to resist antibiotic stress. Additionally, it will delve into how Nucleolar-associated proteins in bacteria perform histone functions akin to eukaryotes. Epigenetics, a non-classical regulatory mechanism of bacterial resistance, may offer new avenues for antibiotic target selection and the development of novel antibiotics.

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Section: Bacterial Epigenetics Mediating Antibiotic Resistancementioning

confidence: 99%

Antimicrobial resistance and mechanisms of epigenetic regulation

Wang

2023

Front. Cell. Infect. Microbiol.

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“…Hfq regulates the expression of T3SS regulator VirB and a mutant exhibits reduced virulence and antibiotic resistance [ 148 ]. The transcriptomic study of this regulator revealed a genetic network that requires further characterization [ 149 ].…”

Section: Pathogenesis Of Shigella Sppmentioning

confidence: 99%

Pathogenicity and virulence of Shigella sonnei : A highly drug-resistant pathogen of increasing prevalence

Matanza,

Clements

2023

Virulence

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Shigella spp. are the causative agent of shigellosis (or bacillary dysentery), a diarrhoeal disease characterized for the bacterial invasion of gut epithelial cells. Among the 4 species included in the genus, Shigella flexneri is principally responsible for the disease in the developing world while Shigella sonnei is the main causative agent in high-income countries. Remarkably, as more countries improve their socioeconomic conditions, we observe an increase in the relative prevalence of S. sonnei . To date, the reasons behind this change in aetiology depending on economic growth are not understood. S. flexneri has been widely used as a model to study the pathogenesis of the genus, but as more research data are collected, important discrepancies with S. sonnei have come to light. In comparison to S. flexneri, S. sonnei can be differentiated in numerous aspects; it presents a characteristic O-antigen identical to that of one serogroup of the environmental bacterium Plesiomonas shigelloides , a group 4 capsule, antibacterial mechanisms to outcompete and displace gut commensal bacteria, and a poorer adaptation to an intracellular lifestyle. In addition, the World Health Organization (WHO) have recognized the significant threat posed by antibiotic-resistant strains of S. sonnei , demanding new approaches. This review gathers knowledge on what is known about S. sonnei within the context of other Shigella spp. and aims to open the door for future research on understanding the increasing spread of this pathogen.

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“…Due to the diversity of their targets, sRNAs and Hfq are involved in numerous bacterial pathways, including virulence and pathogenicity [ 15 , 16 ]. For this reason, Hfq has attracted considerable attention [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

Turbant,

Esnouf,

Rosaz

et al. 2023

Microorganisms

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Due to their two-cell membranes, Gram-negative bacteria are particularly resistant to antibiotics. Recent investigations aimed at exploring new target proteins involved in Gram-negative bacteria adaptation helped to identify environmental changes encountered during infection. One of the most promising approaches in finding novel targets for antibacterial drugs consists of blocking noncoding RNA-based regulation using the protein cofactor, Hfq. Although Hfq is important in many bacterial pathogens, its involvement in antibiotics response is still unclear. Indeed, Hfq may mediate drug resistance by regulating the major efflux system in Escherichia coli, but it could also play a role in the influx of antibiotics. Here, using an imaging approach, we addressed this problem quantitatively at the single-cell level. More precisely, we analyzed how Hfq affects the dynamic influx and efflux of ciprofloxacin, an antibiotic from the group of fluoroquinolones that is used to treat bacterial infections. Our results indicated that the absence of either whole Hfq or its C-terminal domain resulted in a more effective accumulation of ciprofloxacin, irrespective of the presence of the functional AcrAB-TolC efflux pump. However, overproduction of the MicF small regulatory RNA, which reduces the efficiency of expression of the ompF gene (coding for a porin involved in antibiotics influx) in a Hfq-dependent manner, resulted in impaired accumulation of ciprofloxacin. These results led us to propose potential mechanisms of action of Hfq in the regulation of fluoroquinolone fluxes across the E. coli envelope.

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Involvement of RNA chaperone hfq in the regulation of antibiotic resistance and virulence in Shigella sonnei

Cited by 6 publications

References 55 publications

Antimicrobial resistance and mechanisms of epigenetic regulation

Antimicrobial resistance and mechanisms of epigenetic regulation

Pathogenicity and virulence of Shigella sonnei : A highly drug-resistant pathogen of increasing prevalence

Role of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes

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