Involvement of sulphur nutrition in modulating iron deficiency responses in photosynthetic organelles of oilseed rape (Brassica napus L.)

Abstract

The aim of this study was to characterize the roles of sulphur (S) nutrition in modulating the responses to iron (Fe) deficiency in the photosynthetic organelles of oilseed rape. Eight-week-old plants grown hydroponically were fed with S-sufficient or S-deprived solution with or without Fe(III)-EDTA. Responses to four S and Fe combined treatments were analysed after 5 and 10 days. Leaf chlorosis was generated by either S- or Fe-deprivation, with a decrease in chlorophyll and carotenoid content. These negative effects were more severe in the absence of S. The expression of Fe²⁺ transporter (IRT1) and Fe(III) chelate reductase (FRO1) gene was induced for the first 5 days and decreased after 10 days in the S-deprived roots, but largely improved by S supply even in the absence of Fe. Lack of ferric chelate reducing activity in the Fe-deprived roots in the absence of S was largely improved by S supply. The activity of photosynthesis, RuBisCO and sucrose synthase was closely related to S status in leaves. Electron microscopic observation showed that the Fe-deficiency in the absence of S greatly resulted in a severe disorganisation of thylakoid lamellae with loss of grana. However, these impacts of Fe-deficiency were largely restored in the presence of S. The present results indicate that S nutrition has significant role in ameliorating the damages in photosynthetic apparatus caused by Fe-deficiency.

Sulfate-mediated Drought Tolerance in Maize Involves Regulation at Physiological and Biochemical Levels

“…A, E, g s and C i is generally considered to be the first effects of drought stress due to chlorophyll degradation and restriction in available CO 2 68,69 . Reduced gas exchange under S deficient conditions has been previously reported in several plant species such as barley 70 , mustard 71 and rape 72 . In this study, application of S fertilizers considerably (P < 0.001) increased A (Fig.…”

Sulfur Metabolism and Stress Defense Responses in Plants

“…Another interesting aspect is that Fe-deficiency can result in severe disruption to the thylakoid lamellae, with loss of grana, but such damage to the photosynthetic apparatus can be diminished by S nutrition. Photosynthetic activity and sucrose synthase and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO, EC 4.1.1.39) activity are also closely related to S status (Muneer et al 2014).…”

Response of Rice Yield and Soil to Sulfur Application under Water and Salinity Stresses

“…S-deficiency results in general inhibition of photosynthesis and protein synthesis (Dubousset et al, 2010). S-deficiency reduces chlorophyll and rubisco content , and provokes chlorosis of young leaves (Muneer et al, 2014). Sulfur application decreased the hazardous effect of salinity and had favorable effect on growth and nutrient contents of rice.…”