Involvement of the 90 kDa glycoprotein in adhesion ofNectria haematococcamacroconidia

Kwon, Young Hye; Epstein, Lynn

doi:10.1006/pmpp.1997.0114

Cited by 24 publications

(8 citation statements)

References 29 publications

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“…Such glycoproteins can probably also be modified after secretion from the cells. For example, it has been suggested that extracellular transglutaminase activity polymerizes the adhesive glycoprotein of H. haematococca (Kwon & Epstein 1997). In the present study, evidence was obtained that germ tube adhesion of B. sorokiniana is mediated by extracellular glycoproteins.…”

Section: Discussionsupporting

confidence: 57%

Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces

Apoga

Jansson

Tunlid

2001

Mycological Research

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Soon after coming in contact with its host, the plant pathogenic fungus Bipolaris sorokiniana produces an extracellular material that appears to be important for adhering conidia and germlings to the host surface. To further understand this step of the infection, the adhesion of B. sorokiniana to artificial solid surfaces was examined. On a hydrophobic (polystyrene) surface adhesion occurred in two stages, the first by conidia and the second by germlings. Conidial adhesion occurred shortly (0-1 h) after hydration. The conidia were easily detached by increasing the shear force and including detergents in the washing buffer. As conidia were hydrophobic, these observations indicate that conidial adhesion to polystyrene is due to weak, hydrophobic interaction. The second stage of adhesion was accompanied by conidial germination and occurred 1-2 h after hydration and contact with the surface. Concomitant with the delayed adhesion, the fungus produced an extracellular matrix (ECM). The adhesion of germlings was firm and surfaceunspecific since they adhered to both hydrophobic and hydrophilic (glass) surfaces. Except for strong bases, hydrochloric acid and broad-specificity proteases (including Pronase E), none of the hydrolytic enzymes, electrolyte solutions, ionic and hydrophobic detergents and organic solvents removed germlings from the solid surfaces. The adhesion of germlings incubated in the presence of the protein glycosylation inhibitor tunicamycin or the lectins Con A (Concanavalin A) and GNA (from Galanthus nivalis) was significantly reduced, which indicates the involvement of surface glycoproteins in this process. The surface proteins of germlings were labelled with "#&I, extracted and analysed by two-dimensional gel electrophoresis. This revealed about 40 surface proteins over a wide pH range (4-10) with molecular masses between 10 and 100 kDa.

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Section: Discussionsupporting

confidence: 57%

Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces

Apoga

Jansson

Tunlid

2001

Mycological Research

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“…This mechanism of adhesion is clearly different to that of some other fungal pathogens in which pre-formed adhesive is secreted ARTICLE IN PRESS on contact with a surface e.g. M. grisea [26] and N. haematococca [45].…”

Section: The Role Of the Spore Coat In Pathogenicitymentioning

confidence: 59%

The spore coat of the bean anthracnose fungus Colletotrichum lindemuthianum is required for adhesion, appressorium development and pathogenicity

Rawlings

O’Connell²,

Green

2007

Physiological and Molecular Plant Pathology

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The spores (conidia) of the bean anthracnose fungal pathogen, Colletotrichum lindemuthianum, adhere to the aerial parts of plants to initiate the infection process. In previous studies we have shown that the Colletotrichum spores are surrounded by a fibrillar spore coat, comprising several major glycoproteins. Previous evidence showed that a monoclonal antibody (UB20) that recognised these glycoproteins was able to inhibit adhesion of spores to a hydrophobic surface. In this paper we have further studied the role of the spore coat in adhesion, germination and fungal development by studying the effects of UB20 and protease treatment of spores. The latter treatment has previously been shown to remove the spore coat. Spores germinate on glass, polystyrene and water agar, however, appressoria only develop on glass or polystyrene, showing a requirement for a hard surface. Removal of the spore coat with protease inhibits adhesion at 30 min, before the secretion of ECM glycoproteins. Protease treatment also inhibits the development of appressoria and reduces pathogenicity on leaves. Incubation of spores with the MAb UB20 inhibits adhesion at 30 min, but does not affect appressorium formation or pathogenicity. The results suggest that an intact spore coat has two functions; it is required for adhesion to a hydrophobic surface and for the detection of a hard surface necessary for appressorium formation. We suggest that contact with a hard surface, rather than adhesion, is the key event leading to appressorium formation. r

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“…Alternatively, the components could bind to the cellÕs ECM, masking its adhesive function either through direct coating or by inhibiting proper assembly of the ECM components. Evidence for such post-release rearrangements of an ECM has been shown in Bipolaris sorokiniana and Nectria haematococca (Apoga and Jansson, 2000;Kwon and Epstein, 1997). Hydrophobins, hydrophobic surface active fungal proteins that facilitate fungal adhesion to hydrophobic surfaces, are also known to assemble after their release from the cell (deVocht et al, 1998;W€ o osten et al, 1994).…”

Section: Discussionmentioning

confidence: 93%

Quantification of substratum contact required for initiation of Colletotrichum graminicola appressoria

Apoga

Barnard

Craighead

et al. 2004

Fungal Genetics and Biology

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Involvement of the 90 kDa glycoprotein in adhesion ofNectria haematococcamacroconidia

Cited by 24 publications

References 29 publications

Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces

Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces

The spore coat of the bean anthracnose fungus Colletotrichum lindemuthianum is required for adhesion, appressorium development and pathogenicity

Quantification of substratum contact required for initiation of Colletotrichum graminicola appressoria

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