Involvement of the Oxidative Burst in Phytoalexin Accumulation and the Hypersensitive Reaction

Devlin, Winifred S.; Gustine, David L.

doi:10.1104/pp.100.3.1189

Cited by 103 publications

(44 citation statements)

References 25 publications

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“…One of the reaction products formed by oxalate oxidase is H 2 O 2 , and the rapid release of H 2 O 2 has been reported to occur in several plants upon treatment with an elicitor of plant defense responses (Apostol et al, 1989;Devlin and Gustine, 1992;Niirnberger et al, 1994). Moreover, Chen et al (1993) recently demonstrated that salicylic acid, a natural signal molecule for the activation of plant defense responses, inhibits a catalase isoenzyme in tobacco leaves, leading to increased levels of H 2 O 2 .…”

Section: Discussionmentioning

confidence: 99%

Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Dumas¹,

Freyssinet²,

Pallett³

1995

Plant Physiol.

170

105

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Oxalate oxidase activity was detected in situ during the development of barley seedlings. The presence of germin-like oxalate oxidase was confirmed by immunoblotting using an antibody directed against wheat germin produced in Escherichia coli, which is shown to cross-react with barley (Hordeum vulgare) oxalate oxidase and by enzymatic assay after electrophoresis of the protein extracts on polyacrylamide gels. In 3-d-old barley seedlings, oxalate oxidase is localized in the epidermal cells of the mature region of primary roots and in the coleorhiza. After 10 d of growth, the activity is detectable only in the coleorhiza. Moreover, we show that oxalate oxidase is induced in barley leaves during infection by the fungus Erysiphe graminis f. sp. hordei but not by wounding. Thus, oxalate oxidase is a new class of proteins that responds to pathogen attack. We propose that oxalate oxidase could have a role in plant defense through the production of H,O,.

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Section: Discussionmentioning

confidence: 99%

Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Dumas¹,

Freyssinet²,

Pallett³

1995

Plant Physiol.

170

105

View full text Add to dashboard Cite

show abstract

“…These AOS include superoxide (O[-), hydrogen peroxid,: (H202), and the hydroxyl radical (OH') [1,2]. The signifivance of AOS acting as a signal towards host defense in plant-pathogen interactions has been demonstrated by HaO2.mediated oxidative cross-linking of the plant cell wall during HR [3,4], phytoalexin formation in cultured cells [5][6][7] and induction of cellular protection genes [4].…”

Section: Introductionmentioning

confidence: 99%

Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme

et al. 1996

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“…Furthermore, the presence of H 2 O 2 in the plant in response to herbivory, before any subsequent secondary pathogen invasion, could be advantageous because timing of the induction of defense responses can be an important factor in the success or failure of plants to defend against pathogen attacks (Dangl et al, 1996), and wound-generated H 2 O 2 that occurs in the veins also could have a defensive role against bacteria, fungi, or viruses, as they may invade leaves wounded by herbivores (Orozco-Cárdenas and Ryan, 1999). ROS also represent second messengers that eventually activate downstream defense reactions (Foyer and Noctor, 2005), such as synthesis of pathogenesis-related proteins (Chen et al, 1993), glutathione S-transferase, glutathione peroxidase (GPX), and ubiquitin (Levine et al, 1994), as well as phytoalexin accumulation (Devlin and Gustine, 1992;Mithöfer et al, 2004) and production of herbivoreinduced volatile products (Mithöfer et al, 2004).…”

mentioning

confidence: 99%

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

et al. 2006

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In response to herbivore (Spodoptera littoralis) attack, lima bean (Phaseolus lunatus) leaves produced hydrogen peroxide (H 2 O 2 ) in concentrations that were higher when compared to mechanically damaged (MD) leaves. Cellular and subcellular localization analyses revealed that H 2 O 2 was mainly localized in MD and herbivore-wounded (HW) zones and spread throughout the veins and tissues. Preferentially, H 2 O 2 was found in cell walls of spongy and mesophyll cells facing intercellular spaces, even though confocal laser scanning microscopy analyses also revealed the presence of H 2 O 2 in mitochondria/peroxisomes. Increased gene and enzyme activations of superoxide dismutase after HW were in agreement with confocal laser scanning microscopy data. After MD, additional application of H 2 O 2 prompted a transient transmembrane potential (V m ) depolarization, with a V m depolarization rate that was higher when compared to HW leaves. In transgenic soybean (Glycine max) suspension cells expressing the Ca 21 -sensing aequorin system, increasing amounts of added H 2 O 2 correlated with a higher cytosolic calcium ([Ca 21 ] cyt ) concentration. In MD and HW leaves, H 2 O 2 also triggered the increase of [Ca 21 ] cyt , but MD-elicited [Ca 21 ] cyt increase was more pronounced when compared to HW leaves after addition of exogenous H 2 O 2 . The results clearly indicate that V m depolarization caused by HW makes the membrane potential more positive and reduces the ability of lima bean leaves to react to signaling molecules.

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Involvement of the Oxidative Burst in Phytoalexin Accumulation and the Hypersensitive Reaction

Abstract: The role of the oxidative burst, transient production of activated oxygen species such as H202 and superoxide (02)

Cited by 103 publications

References 25 publications

Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Tissue-Specific Expression of Germin-Like Oxalate Oxidase during Development and Fungal Infection of Barley Seedlings

Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase‐like enzyme

Effects of Feeding Spodoptera littoralis on Lima Bean Leaves. III. Membrane Depolarization and Involvement of Hydrogen Peroxide

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