Involvement of the Tyr Kinase/JNK Pathway in Carbachol-induced Bronchial Smooth Muscle Contraction in the Rat

Sakai, Hiroyasu; Watanabe, Yu; Honda, Masaaki; Tsuiki, Rika; Ueda, Yusuke; Nagai, Yuki; Narita, Minoru; Misawa, Miwa; Chiba, Yoshihiko

doi:10.1097/aln.0b013e318286d0ae

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Sodium orthovanadate is a well-known tyrosine (Tyr) phosphatase inhibitor that indirectly shifts the kinase-phosphatase balance towards phosphorylation of Tyr kinases. Such findings are in accordance with the lower potency of β-citronellol against contractions evoked by metabotropic cascades if we consider that Tyr kinases have been reported to be downstream pathways in the contractile responses evoked by cholinergic agonists acting through G protein-coupled muscarinic receptors ( 23 ).…”

Section: Resultssupporting

confidence: 87%

β-Citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea

Vasconcelos

Ribeiro-Filho

Lucetti

et al. 2016

Braz J Med Biol Res

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β-Citronellol is an alcoholic monoterpene found in essential oils such Cymbopogon citratus (a plant with antihypertensive properties). β-Citronellol can act against pathogenic microorganisms that affect airways and, in virtue of the popular use of β-citronellol-enriched essential oils in aromatherapy, we assessed its pharmacologic effects on the contractility of rat trachea. Contractions of isolated tracheal rings were recorded isometrically through a force transducer connected to a data-acquisition device. β-Citronellol relaxed sustained contractions induced by acetylcholine or high extracellular potassium, but half-maximal inhibitory concentrations (IC50) for K+-elicited stimuli were smaller than those for cholinergic contractions. It also inhibited contractions induced by electrical field stimulation or sodium orthovanadate with pharmacologic potency equivalent to that seen against acetylcholine-induced contractions. When contractions were evoked by selective recruitment of Ca2+ from the extracellular medium, β-citronellol preferentially inhibited contractions that involved voltage-operated (but not receptor-operated) pathways. β-Citronellol (but not verapamil) inhibited contractions induced by restoration of external Ca2+ levels after depleting internal Ca2+ stores with the concomitant presence of thapsigargin and recurrent challenge with acetylcholine. Treatment of tracheal rings with L-NAME, indomethacin or tetraethylammonium did not change the relaxing effects of β-citronellol. Inhibition of transient receptor potential vanilloid subtype 1 (TRPV1) or transient receptor potential ankyrin 1 (TRPA1) receptors with selective antagonists caused no change in the effects of β-citronellol. In conclusion, β-citronellol exerted inhibitory effects on rat tracheal rings, with predominant effects on contractions that recruit Ca2+ inflow towards the cytosol by voltage-gated pathways, whereas it appears less active against contractions elicited by receptor-operated Ca2+ channels.

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Section: Resultssupporting

confidence: 87%

β-Citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea

Vasconcelos

Ribeiro-Filho

Lucetti

et al. 2016

Braz J Med Biol Res

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“…Moreover, DDT and its metabolite DDE have been shown to be able to activate stress-response signaling in vitro , including ERK/MAPK (extracellular-signal-regulated kinase/mitogen-activated protein kinase), JNK (c-Jun N-terminal kinase), and NF-κB (nuclear factor kappa-light-chain-enhancer of activated B cells) signaling pathways, which results in an intracellular release of calcium ( Abdollahi et al 2004 ; Androutsopoulos et al 2013 ). Calcium release into cytoplasm has also been shown to lead to contraction of airway smooth muscles in studies of airway smooth muscle cells and animal model rat studies ( Berridge 2008 ; Menshikova et al 1995 ; Sakai et al 2013 ; Tomasic et al 1992 ). Airway narrowing in response to DDT/DDE exposures would be consistent with the negative associations between exposures and lung function in our study population.…”

Section: Discussionmentioning

confidence: 99%

Association between Lung Function in Adults and Plasma DDT and DDE Levels: Results from the Canadian Health Measures Survey

Ye¹,

Beach

Martin

2015

Environ Health Perspect

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BackgroundAlthough DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] has been banned in many countries since the 1970s, it may still pose a risk to human respiratory health. In agriculture, DDT exposures have been associated with asthma and chronic bronchitis. However, little is known about the effect of DDT on lung function.MethodsWe used data on 1,696 participants 20–79 years of age from the Canadian Health Measures Survey (CHMS) and conducted multiple regression analysis to estimate associations between plasma p,p´-DDT/DDE and lung function.ResultsAlmost all participants (> 99.0%) had detectable concentrations of plasma p,p´-DDE, but only 10.0% had detectable p,p´-DDT. Participants with detectable p,p´-DDT had significantly lower mean FVC (difference = 311 mL; 95% CI: –492, –130; p = 0.003) and FEV1 (difference = 232 mL; 95% CI: –408, –55; p = 0.015) than those without. A 100-ng/g lipid increase in plasma p,p´-DDE was associated with an 18.8-mL decrease in mean FVC (95% CI: –29, –9) and an 11.8-mL decrease in mean FEV1 (95% CI: –21, –3). Neither exposure was associated with FEV1/FVC ratio or FEF25%–75%.ConclusionsDDT exposures, which may have occurred decades ago, were still detectable among Canadians. Plasma DDT and DDE were negatively associated with lung function parameters. Additional research on the potential effects of DDT use on lung function is warranted.CitationYe M, Beach J, Martin JW, Senthilselvan A. 2015. Association between lung function in adults and plasma DDT and DDE levels: results from the Canadian Health Measures Survey. Environ Health Perspect 123:422–427; http://dx.doi.org/10.1289/ehp.1408217

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Pulmonary fluorosis: a review

Ameeramja

Perumal

2017

Environ Sci Pollut Res

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The increased industrialization and improvised human lifestyle lead to a surge in environmental pollution nowadays. Even the chemicals which are known as prophylactic agents were currently liable to be toxic. One among them is inorganic fluoride whose wider application in numerous processes makes it as an inevitable environmental contaminant and industrial pollutant. Although the systemic toxicity of fluoride has been extensively studied, still there is lacuna in the field of pulmonary fluoride toxicity. Hence, we have focused on the molecular mechanism of action of fluoride compounds on pulmonary system. A study of literatures that focused on the potential physiological and toxicological consequences of fluoride on pulmonary system was carried out. The goal of this review is to present an overview of the research carried out till date on the molecular aspects of fluoride exposure with emphasis on pulmonary system and their possible mechanisms.

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Involvement of the Tyr Kinase/JNK Pathway in Carbachol-induced Bronchial Smooth Muscle Contraction in the Rat

Cited by 4 publications

References 25 publications

β-Citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea

β-Citronellol, an alcoholic monoterpene with inhibitory properties on the contractility of rat trachea

Association between Lung Function in Adults and Plasma DDT and DDE Levels: Results from the Canadian Health Measures Survey

Pulmonary fluorosis: a review

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