Involvement of the V2 Vasopressin Receptor in Adaptation to Limited Water Supply

Böselt, Iris; Römpler, Holger; Hermsdorf, Thomas; Thor, Doreen; Busch, Wibke; Schulz, Angela; Schöneberg, Torsten

doi:10.1371/journal.pone.0005573

Cited by 33 publications

(35 citation statements)

References 54 publications

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“…The human V1A, V1B and OT receptors are encoded by two exons while the human V2 receptor (V2A) is encoded by three [43] . The first intron of the human V2A receptor gene is suggested to be a mammal-specific insertion while the second intron is an ancestral intron found in all vertebrate OT/VP receptors [8] . Our findings support this view.…”

Section: Discussionmentioning

confidence: 99%

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Daza¹,

Lewicka²,

Larhammar³

2012

General and Comparative Endocrinology

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Section: Discussionmentioning

confidence: 99%

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Daza¹,

Lewicka²,

Larhammar³

2012

General and Comparative Endocrinology

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“…Transient co-transfection experiments of COS-7 cells with the GPR34 constructs and the chimeric G protein G␣ qi4 and inositol phosphate accumulation assays (26) were essentially performed as described previously (9). All GPR34 constructs were introduced into the mammalian expression vector pcDps and double-tagged with an N-terminal HA tag and a C-terminal FLAG tag to monitor and quantify total cellular and plasma membrane expression using ELISA (27). Correctness of all PCR-derived constructs was verified by sequencing.…”

Section: Methodsmentioning

confidence: 99%

Altered Immune Response in Mice Deficient for the G Protein-coupled Receptor GPR34

Liebscher¹,

Müller

Teupser

et al. 2011

Journal of Biological Chemistry

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103

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The X-chromosomal GPR34 gene encodes an orphan G i protein-coupled receptor that is highly conserved among vertebrates. To evaluate the physiological relevance of GPR34, we generated a GPR34-deficient mouse line. GPR34-deficient mice were vital, reproduced normally, and showed no gross abnormalities in anatomical, histological, laboratory chemistry, or behavioral investigations under standard housing. Because GPR34 is highly expressed in mononuclear cells of the immune system, mice were specifically tested for altered functions of these cell types. Following immunization with methylated BSA, the number of granulocytes and macrophages in spleens was significantly lower in GPR34-deficient mice as in wild-type mice. GPR34-deficient mice showed significantly increased paw swelling in the delayed type hypersensitivity test and higher pathogen burden in extrapulmonary tissues after pulmonary infection with Cryptococcus neoformans compared with wild-type mice. The findings in delayed type hypersensitivity and infection tests were accompanied by significantly different basal and stimulated TNF-␣, GM-CSF, and IFN-␥ levels in GPR34-deficient animals. Our data point toward a functional role of GPR34 in the cellular response to immunological challenges. G protein-coupled receptors (GPCR)2 form the largest gene family among transmembrane receptors, including more than 900 genes in humans and other mammals (1). A great number of stimuli, such as light, hormones, neurotransmitters, peptides, and nucleotides, activate the distinct receptors. Nonodorant receptors form about one-third of the GPCR repertoire. Although more than 200 non-odorant GPCR have been assigned to specific agonists and functions, about 155 socalled "orphan" GPCR (2) await identification of their physiological relevance. The importance of GPCR in controlling almost every physiological function makes this receptor family the most frequently used target for therapeutic drugs. Therefore, unveiling the function of orphan GPCR is a central issue in academic and industrial research.Among the five structurally different GPCR families (1, 3), the rhodopsin-like receptors form the largest in humans and other vertebrates. The rhodopsin-like family is divided further into subfamilies and groups. The P2Y 12 -like receptor group includes the ADP receptors P2Y 12 and P2Y 13 , the UDP-glucose receptor P2Y 14 , and the orphan receptors GPR87, GPR82, and GPR34 (4). Apart from the ADP receptor P2Y 12 , which has a central role in platelet aggregation and is the therapeutic target of clopidogrel (5, 6), very little is known about the function of the other members of this group.GPR34, an orphan receptor of the P2Y 12 -like receptor group, was first discovered by mining GenBank TM for novel GPCR sequences and homology cloning and has been assigned to the human X chromosome (7,8). Phylogenetic studies revealed that GPR34 has been highly conserved over the past 450 million years of vertebrate evolution, and no GPR34-deficient vertebrate has been identified yet (9). To date, there i...

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“…1, 2, 3). In contrast, the ICL III of the V2R shows high variability among vertebrates in its length and amino acid composition (Böselt et al 2009). The ICL III of the (Lema 2010;Konno et al 2010) has nearly identical structure to that of the catfish V2R.…”

Section: Structural Characteristics Of the Putative Vt Receptorsmentioning

confidence: 99%

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

Rawat

Chaube

Joy

2015

Fish Physiol Biochem

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Vasotocin (VT) is the ortholog of vasopressin (VP) in non-mammalian vertebrates and is known for multiple functions. Teleost fishes have a complete repertoire of known VP/VT receptor subtypes (vasopressin type, VR): two V1A subtypes (V1Aa and V1Ab or V1a1 and V1a2) and five V2 subtypes (V2A1, V1A2, V2B1, V2B2 and V2C). Full-length cDNAs of v1a1, v1a2 and v2 (v2a1) with ORFs of 1,308, 1,137 and 1,527 bp, respectively, were cloned and characterized in the catfish Heteropneustes fossilis (Siluriformes, Ostariophysi). BLAST analysis revealed that the genes encoded three VT receptors, V1a1, V1a2 and V2 of 436, 379 and 509 amino acid residues, respectively. The predicted proteins showed typical features of the seven-transmembrane domain receptor core structure with hallmark triplets Asp-Arg-Tyr/Asp-Arg-His (DRY/DRH) and the variable intracellular loop III of vertebrate neurohypophysial hormone receptors. Phylogenetic analysis of the deduced protein sequences revealed that they clustered with the V1Aa, V1Ab and V2A1, respectively, of other teleosts. The V2R has a sequence identity of 70-76% with V2A1 than with the V2B type (sequence identity 43-49%). Semiquantitative PCR analysis showed that the receptor gene transcripts were expressed ubiquitously in the tissues examined (brain, pituitary, gonads, liver, muscle, kidney and gills) and displayed sex and seasonal fluctuations in a tissue-specific manner. The results form a basis for functional studies on the VT receptors in the catfish.

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Involvement of the V2 Vasopressin Receptor in Adaptation to Limited Water Supply

Cited by 33 publications

References 54 publications

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

The oxytocin/vasopressin receptor family has at least five members in the gnathostome lineage, inclucing two distinct V2 subtypes

Altered Immune Response in Mice Deficient for the G Protein-coupled Receptor GPR34

Molecular cloning, sequencing and phylogeny of vasotocin receptor genes in the air-breathing catfish Heteropneustes fossilis with sex dimorphic and seasonal variations in tissue expression

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