Involvement of TSP1 and MMP9/NGAL in Angiogenesis during Orthodontic Periodontal Remodeling

Abstract

In the present study the aim was to measure the levels of Thrombospondin-1 (TSP1) and Lipocalin-2/matrix metalloproteinase 9 (MMP9/NGAL) complex in gingival crevicular fluid (GCF) at different time points of orthodontic treatment, to determine the relationship between these values and those of total-matrix metalloproteinase 9 (MMP9) and theirs implication in angiogenesis balance, in the situation of a good control of the bacterial plaque, emphasizing the role of TSP1 and MMP9/NGAL complex. GCF samples were collected from 16 young orthodontic patients requiring upper canine distalization (test tooth) with first premolar extraction. The contralateral canine (control tooth) was free from orthodontic force. For the orthodontic appliance, brackets Roth 0.018 inch with 0.012 inch NiTi archwire and a laceback were used. TSP1, MMP9/NGAL, and MMP9 increased from 1 hour before activation of orthodontic appliance to a maximum at 8 hours for MMP9 and 72 hours for MMP9/NGAL and TSP1. The results show a change in time of TSP1, MMP9/NGAL, and MMP9 levels in GCF of patients with this method of orthodontic treatment. The powerful correlation of MMP9/NGAL with TSP1 suggests their stronger involvement in angiogenesis processes in PDL during orthodontic periodontal remodeling, in the situation of a healthy periodontium and a good control of the bacterial plaque.

Global proteome profiling of dental cementum under experimentally-induced apposition

“…THBS1 is expressed in osteoblasts [52, 53] and odontoblasts [54], especially during early stages of differentiation and mineralization [54, 55]. Interestingly, THBS1 was up-regulated in periodontia under orthodontic treatment [56], indicating a potential function in remodeling periodontal tissues.…”

Preformed gelatin microcryogels as injectable cell carriers for enhanced skin wound healing

“…MMP8 and MMP9 also play positive role in angiogenesis process [37,38]. However, contradicting findings demonstrated the complex role of MMP-8 in tissue repair [49].…”

“…VEGF and HGF secretion were significantly enhanced in GMs + hASCs group. Meanwhile, matrix metalloproteinases MMP8 and MMP9, bioactive factors involved in angiogenesis [37,38], were found significantly increased in GMs + hASCs group. Secretion of EGF and FGF2 exhibited no significant differences.…”

Periodontal Changes and Oral Health

“…In studies funded by the grant ID573/2008 of the Ministry of Education and Research of Romania, we measured the levels of Pentraxin-3 (PTX3), Thrombospondin1 (TSP1), Lipoca-lin2/Matrix metalloproteinase 9 (MMP9/NGAL) complex and Matrix metalloproteinase 9 (MMP9) in GCF at different time points of the first 2 weeks of orthodontic treatment, to determine the relationship between these values and theirs implication in inflammation and angiogenesis balance, in the situation of a good control of the bacterial plaque [74,75].…”