Involvements of PHI-nitric oxide and PACAP-BK channel in the sustained relaxation of mouse gastric fundus

Hagi, Kiyomi; Azuma, Yasutaka; Nakajima, Hidemitsu; Shintani, Norihito; Hashimoto, Hitoshi; Baba, Akemichi; Takeuchi, Tadayoshi

doi:10.1016/j.ejphar.2008.05.045

Cited by 15 publications

(16 citation statements)

References 42 publications

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“…For example, PACAP has various hormonal functions in all endocrine glands; it influences motility and secretion in the gastrointestinal and respiratory tracts and regulates urogenital physiology (Vaudry et al 2000 ;Cardell et al 1991 ;Cheng et al 1993 ;Ishizuka et al 1992 ;Botz et al 2013 ). In addition, it has been revealed that PACAP has potent vasomotor effects Nandha et al 1991 ;Hagi et al 2008 ). Several human studies demonstrate that PACAP exerts cardiovascular actions partially by the decrease in mean arterial blood pressure and an increase in blood flow, measured invasively on beagle dog femoral arteries (Naruse et al 1993 ;Whalen et al 1999 ).…”

Section: Pacap-induced Vasomotor Responses Of Various Tissuesmentioning

confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Induces Relaxations of Peripheral and Cerebral Arteries, which are Differentially Impaired by Aging

et al. 2014

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Section: Pacap-induced Vasomotor Responses Of Various Tissuesmentioning

confidence: 99%

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Induces Relaxations of Peripheral and Cerebral Arteries, which are Differentially Impaired by Aging

et al. 2014

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“…The extent of relaxation was expressed as the area under the line of resting tone that was drawn on the bottom of resting spontaneous contractile activity (broken lines in Fig. 1) as described elsewhere (28). Relaxing responses to NOR-1, a NO donor, and carbon monoxide-releasing molecule-2 (CORM-2) were conducted before and after the application of different antagonists used.…”

Section: Recording Of Responses Of Longitudinal Muscle To Efs and Agomentioning

confidence: 99%

Nitric Oxide and Carbon Monoxide Act as Inhibitory Neurotransmitters in the Longitudinal Muscle of C57BL/6J Mouse Distal Colon

et al. 2010

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Abstract. The present study was designed to identify the inhibitory neurotransmitters mediating nonadrenergic noncholinergic relaxation in the longitudinal muscle of C57/BL mouse distal colon. Relaxation induced by electrical field stimulation (EFS) was recorded isotonically in the presence of atropine and guanethidine. Cyclic guanosine-3′,5′-monophosphate (cyclic GMP) content was measured by radioimmunoassay. EFS-induced relaxation was inhibited by nitro-L -arginine ( L -NNA) and Sn (IV) protoporphyrin dichloride IX (SnPP-IX), a nitric oxide (NO) and carbon monoxide (CO) synthase inhibitor, respectively. A combination of both inhibitors produced an additive effect. ODQ, a soluble guanylate cyclase inhibitor, inhibited EFS-induced relaxation. NOR-1, a NO donor, and carbon monoxide-releasing molecule-2 (CORM-2), a CO donor, treatment relaxed the distal colon and increased cyclic GMP content. The effects of NOR-1 and CORM-2 were inhibited by ODQ. KT5823, a cyclic GMP-dependent protein kinase inhibitor, inhibited EFS-induced relaxation. EFS-induced relaxation in the presence of KT5823 was further inhibited by L -NNA, but not by SnPP-IX. In addition, KT5823 inhibited CORM-2-induced relaxation, but not NOR-1-induced relaxation. H89, a cyclic AMP-dependent protein kinase inhibitor, inhibited EFS-induced relaxation, and EFS-induced relaxation in the presence of H89 was further inhibited by L -NNA. These results suggested that NO and CO function as inhibitory neurotransmitters in the longitudinal muscle of C57BL mouse distal colon.

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“…A relaxation study was carried out using a previously described method [8] . Briefly, the mouse distal colon was removed and placed in Tyrode solution.…”

Section: Relaxation Studiesmentioning

confidence: 99%

Clofibrate Relaxes the Longitudinal Smooth Muscle of the Mouse Distal Colon through Calcium-Mediated Desensitisation of Contractile Machinery

et al. 2011

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Peroxisome proliferator-activated receptor α (PPAR-α) is a ligand-activated transcription factor that exerts strong effects on metabolic pathways. Our aim was to elucidate the effect of clofibrate, a PPAR-α agonist, on the longitudinal muscle of the mouse distal colon. We initially found that clofibrate induced a relaxation response in this muscle. Notably, the PPAR-α antagonists GW9662 and T0070907 did not attenuate this clofibrate-induced relaxation. The structurally related PPAR-α agonists fenofibrate and bezafibrate induced relaxation in the distal colon as effectively as clofibrate. In contrast, wy-14643, which activates PPAR-α more selectively than clofibrate, had no effect. Furthermore, clofibrate-induced relaxation was not affected by N-nitro-L-arginine, an NO synthase inhibitor, 1H-[1,2,4]-oxadiazolo-[4,3- a]quinoxaline-1-one, a soluble guanylate cyclase inhibitor, or H89, a protein kinase A inhibitor. Tetrodotoxin, an Na⁺ channel blocker, and glibenclamide, apamin, charybdotoxin and XE991, various K⁺ channel blockers, had no effect on clofibrate-induced relaxation. Importantly, clofibrate induced a relaxation response that was not accompanied by any alteration in the cytoplasmic Ca²⁺ concentration in the longitudinal muscle of the mouse distal colon. Moreover, calyculin A, a myosin light-chain phosphatase (MLCP) inhibitor, attenuated clofibrate-induced relaxation. Our findings indicate that clofibrate relaxes the longitudinal smooth muscle of the mouse distal colon by regulating MLCP activity.

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Involvements of PHI-nitric oxide and PACAP-BK channel in the sustained relaxation of mouse gastric fundus

Cited by 15 publications

References 42 publications

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Induces Relaxations of Peripheral and Cerebral Arteries, which are Differentially Impaired by Aging

Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Induces Relaxations of Peripheral and Cerebral Arteries, which are Differentially Impaired by Aging

Nitric Oxide and Carbon Monoxide Act as Inhibitory Neurotransmitters in the Longitudinal Muscle of C57BL/6J Mouse Distal Colon

Clofibrate Relaxes the Longitudinal Smooth Muscle of the Mouse Distal Colon through Calcium-Mediated Desensitisation of Contractile Machinery

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