Iodide protection from UVB irradiation-induced degradation of hyaluronate and against UVB-damage of human conjunctival fibroblasts

Schmut, O.; Horwath‐Winter, Jutta; Rieger, G.; Winkler, Rudolf; Trummer, Gabriele; Spitzenberger, Helga; Wachswender, C.

doi:10.1007/s00417-003-0829-z

Cited by 12 publications

(7 citation statements)

References 35 publications

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“…The effect of UV irradiation on keratinocyte CD44 expression might be due either to a direct inhibitory effect of UV on CD44 gene transcription or to a presently unknown feedback mechanism in order to downregulate the amount of cell surface CD44 for HA already decreased in inter‐keratinocyte spaces. It has been shown that UVB degrades HA in cultured human conjunctival fibroblasts (33). Decrease of epidermal HA following UV irradiation might also be explained by degradation of free HA as a result of downregulation of keratinocyte CD44, which, under physiological conditions, binds and holds HA in interkeratinocyte spaces.…”

Section: Discussionmentioning

confidence: 99%

UVA and UVB Decrease the Expression of CD44 and Hyaluronate in Mouse Epidermis, which is Counteracted by Topical Retinoids

Çalıkoğlu

Sorg

Tran

et al. 2006

Photochem & Photobiology

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The transmembrane glycoprotein CD44 is currently thought to be the main cell surface receptor for the glycosaminoglycan hyaluronate. We previously showed that (1) CD44 regulate keratinocyte proliferation; (2) topical retinoids dramatically increase the expression of CD44, hyaluronate and hyaluronate synthase (HAS)s in mouse epidermis; (3) topical retinaldehyde restores the epidermal thickness and CD44 expression which are correlated with clinical improvement in lichen sclerosus et atrophicus lesions; and (4) retinaldehyde-induced proliferative response of keratinocytes is a CD44-dependent phenomenon and requires the presence of HB-EGF, erbB1 and matrix metalloproteinases. In this study, we analyzed the effect of UV irradiation on the levels of epidermal hyaluronate and CD44 in mice, as well as its potential prevention by topical retinoids. UVA (10 J/cm(2)) or UVB (1 J/cm(2)) irradiation significantly decreased the expression of CD44 and hyaluronate in the epidermis of hairless mice after 2 h. Expression of both epidermal CD44 and hyaluronate was reconstituted within 24 h. Topical application of retinaldehyde for 3 days prior to UVA or UVB irradiation prevented the decrease of CD44 and hyaluronate expression. Topical retinol and retinoic acid also increased the basal levels of epidermal CD44 and hyaluronate, although their preventive effect on UV-induced decrease of these molecules was less pronounced as compared to topical retinaldehyde. These data confirm the relationships between retinoid and CD44 pathways, although the primary target(s) of UV leading to CD44 and hyaluronate degradation remain to be elucidated.

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Section: Discussionmentioning

confidence: 99%

UVA and UVB Decrease the Expression of CD44 and Hyaluronate in Mouse Epidermis, which is Counteracted by Topical Retinoids

Çalıkoğlu

Sorg

Tran

et al. 2006

Photochem & Photobiology

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“…55 56 Iodide was shown to provide significant protection for hyaluronate, a component of tear fluid and tissues of the anterior part of the eye against ultraviolet B light induced oxidative degradation. 57 Also, injury to human conjunctival cells can be prevented by incubation with iodide before ultraviolet B irradiation. 57 Peroxidase activity, which can scavenge H 2 O 2 in the presence of a suitable substrate, has been detected in human tears.…”

Section: Discussionmentioning

confidence: 99%

“…57 Also, injury to human conjunctival cells can be prevented by incubation with iodide before ultraviolet B irradiation. 57 Peroxidase activity, which can scavenge H 2 O 2 in the presence of a suitable substrate, has been detected in human tears. 5 Iodide has been found to increase peroxidase activity.…”

Section: Discussionmentioning

confidence: 99%

Iodide iontophoresis as a treatment for dry eye syndrome

Horwath‐Winter

Schmut²,

Em³

et al. 2005

British Journal of Ophthalmology

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Background/aims: Among the causes related to the development or perpetuation and aggravation of dry eye disease, oxidative reactions may have a role in the pathogenesis of this disorder. Antioxidants, such as iodide, have shown a strong effect in preventing the oxidative damage to constituents of the anterior part of the eye. In this clinical trial the effectiveness of iodide iontophoresis and iodide application without current in moderate to severe dry eye patients was compared. Methods: 16 patients were treated with iodide iontophoresis and 12 patients with iodide application without current for 10 days. Subjective improvement, frequency of artificial tear application, tear function parameters (break up time, Schirmer test without local anaesthesia), vital staining (fluorescein and rose bengal staining) as well as impression cytology of the bulbar conjunctiva were evaluated before treatment, 1 week, 1 month, and 3 months after treatment. Results: A reduction in subjective symptoms, frequency of artificial tear substitute application, and an improvement in certain tear film and ocular surface factors could be observed in both groups. A stronger positive influence was seen after application of iodide with current (iontophoresis), as observed in a distinct improvement in break up time, fluorescein and rose bengal staining, and in a longer duration of this effect compared with the non-current group. No significant change in Schirmer test results and impression cytology were observed in both groups. Conclusions: Iodide iontophoresis has been demonstrated to be a safe and well tolerated method of improving subjective and objective dry eye factors in patients with ocular surface disease.

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“…Environmental influences (eg, air pollution, UV-B light) lead to oxidative reactions and consequently damage the tear film. 24 The increase in inflammatory activity may result in an increase in nerve excitation and therefore explain the highest sensitivity values in this subgroup.…”

Section: Cos and Etiology Of Dry Eyementioning

confidence: 99%

Fluctuations of Corneal Sensitivity in Dry Eye Syndromes—A Longitudinal Pilot Study

Nepp

Wirth

2015

Cornea

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Iodide protection from UVB irradiation-induced degradation of hyaluronate and against UVB-damage of human conjunctival fibroblasts

Cited by 12 publications

References 35 publications

UVA and UVB Decrease the Expression of CD44 and Hyaluronate in Mouse Epidermis, which is Counteracted by Topical Retinoids

UVA and UVB Decrease the Expression of CD44 and Hyaluronate in Mouse Epidermis, which is Counteracted by Topical Retinoids

Iodide iontophoresis as a treatment for dry eye syndrome

Fluctuations of Corneal Sensitivity in Dry Eye Syndromes—A Longitudinal Pilot Study

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