Ion beam irradiation mutagenesis in rye (Secale cereale L.), linseed (Linum usitatissimum L.) and faba bean (Vicia faba L.)

Khazaei, Hamid; Mäkelä, Pirjo; Stoddard, Frederick L.

doi:10.23986/afsci.70780

Cited by 11 publications

(7 citation statements)

References 30 publications

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“…Physical (e.g., gamma-ray) and chemical (e.g., ethyl methane sulphonate-EMS) mutagens have demonstrated their usefulness in faba bean mutation induction (Abdalla, 1982;Duc, 1995;Khursheed et al, 2018Khursheed et al, , 2019Nurmansyah et al, 2019Nurmansyah et al, , 2020. Ion beam irradiation has also been reported as an effective and unique technique for inducing mutations in faba bean (Khazaei et al, 2018b). The first mutagenesis on faba bean was reported by Sjödin (1971) with the description of determinate mutants, ti1 and ti2 which significantly reduced the number of flowering nodes giving the plant type as "topless."…”

Section: Mutagenesis In Faba Beanmentioning

confidence: 99%

Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

et al. 2021

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Faba bean is a cool-season grain legume crop, which is grown worldwide for food and feed. Despite a decrease in area under faba bean in the past, the interest in growing faba bean is increasing globally due to its high seed protein content and its excellent ecological service. The crop is, however, exposed to diverse biotic and abiotic stresses causing unstable, low grain yield. Although, sources of resistance to main diseases, such as ascochyta blight (Ascochyta fabae Speg.), rust (Uromyces viciae-fabae (Pers.) Schroet.), chocolate spot (Botrytis fabae Sard.) and gall disease (Physioderma viciae), have been identified, their resistance is only partial and cannot prevent grain yield losses without agronomical practices. Tightly associated DNA markers for host plant resistance genes are needed to enhance the level of resistance. Less progress has been made for abiotic stresses. Different breeding methods are proposed, but until now line breeding, based on the pedigree method, is the dominant practice in breeding programs. Nonetheless, the low seed multiplication coefficient and the requirement for growing under insect-proof enclosures to avoid outcrossing hampers breeding, along with the lack of tools such as double haploid system and cytoplasmic male sterility. This reduces breeding population size and speed of breeding hence the chances of capturing rare combinations of favorable alleles. Availability and use of the DNA markers such as vicine-convicine (vc−) and herbicide tolerance in breeding programs have encouraged breeders and given confidence in marker assisted selection. Closely linked QTL for several biotic and abiotic stress tolerance are available and their verification and conversion in breeder friendly platform will enhance the selection process. Recently, genomic selection and speed breeding techniques together with genomics have come within reach to accelerate the genetic gains in faba bean. Advancements in genomic resources with other breeding tools, methods and platforms will enable to accelerate the breeding process for enhancing genetic gain in this species.

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Section: Mutagenesis In Faba Beanmentioning

confidence: 99%

Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

et al. 2021

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“…Considerable DNA alternations, point-like mutations and null mutations have been observed in mutants of chrysanthemum, carnation and Arabidopsis induced by ion beams (Tanaka et al ., 2010). Appropriate doses of ion beam radiation have also been utilized to generate novel mutants of crops, such as faba bean, rye, rice and linseed (Yamaguchi et al ., 2009; Khazaei et al ., 2018).…”

Section: Experiments In Space and Ground-based Space Simulation Facilmentioning

confidence: 99%

How the space environment influences organisms: an astrobiological perspective and review

Prasad

Richter

Vadakedath

et al. 2021

International Journal of Astrobiology

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The unique environment of space is characterized by several stress factors, including intense radiation, microgravity, high vacuum and extreme temperatures, among others. These stress conditions individually or in-combination influence genetics and gene regulation and bring potential evolutionary changes in organisms that would not occur under the Earth's gravity regime (1 × g). Thus, space can be explored to support the emergence of new varieties of microbes and plants, that when selected for, can exhibit increased growth and yield, improved resistance to pathogens, enhanced tolerance to drought, low nutrient and disease, produce new metabolites and others. These properties may be more difficult to achieve using other approaches under 1 × g. This review provides an overview of the space microgravity and ionizing radiation conditions that significantly influence organisms. Changes in the genomics, physiology, phenotype, growth and metabolites of organisms in real and simulated microgravity and radiation conditions are illustrated. Results of space biological experiments show that the space environment has significant scientific, technological and commercial potential. Combined these potentials can help address the future of life on Earth, part of goal e of astrobiology.

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“…Energy deposition by heavy-ion beams causes double strand breaks in the DNA, base substitutions, and small deletions/insertions [34]. Another technique is the use of the low energy nitrogen (N + ) ions, which damage only the microbial cell surface, and then the ion implantation generates free radicals inside the cell causing DNA damage and a high mutation rate [35,36]. In addition, gamma radiation from radioactive cobalt 60 Co possesses strong penetration power and results in reactive oxygen species (ROS) generation, such as free radicals (O 2 − and OH . )…”

Section: Use Of Random Mutagenesismentioning

confidence: 99%

Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

2020

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Oleaginous microalgae and yeasts represent promising candidates for large-scale production of lipids, which can be utilized for production of drop-in biofuels, nutraceuticals, pigments, and cosmetics. However, low lipid productivity and costly downstream processing continue to hamper the commercial deployment of oleaginous microorganisms. Strain improvement can play an essential role in the development of such industrial microorganisms by increasing lipid production and hence reducing production costs. The main means of strain improvement are random mutagenesis, adaptive laboratory evolution (ALE), and rational genetic engineering. Among these, random mutagenesis and ALE are straight forward, low-cost, and do not require thorough knowledge of the microorganism’s genetic composition. This paper reviews available mutagenesis and ALE techniques and screening methods to effectively select for oleaginous microalgae and yeasts with enhanced lipid yield and understand the alterations caused to metabolic pathways, which could subsequently serve as the basis for further targeted genetic engineering.

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Ion beam irradiation mutagenesis in rye (Secale cereale L.), linseed (Linum usitatissimum L.) and faba bean (Vicia faba L.)

Cited by 11 publications

References 30 publications

Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

Conventional and Molecular Breeding Tools for Accelerating Genetic Gain in Faba Bean (Vicia Faba L.)

How the space environment influences organisms: an astrobiological perspective and review

Harnessing the Power of Mutagenesis and Adaptive Laboratory Evolution for High Lipid Production by Oleaginous Microalgae and Yeasts

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