Ion-channel sensors

DNA-Immobilized Gold Electrode for DNA-Binding Drug Sensor

“…The binding of the cationic drug to the immobilized polyanionic host (i.e., DNA) should reduce the negative charge on the electrode surface, resulting in the enhancement of the current intensity. 6 Such concentration-dependent changes in CV profiles on the DNA-immobilized electrode were not seen for Na+ ion in the concentration range of 10-' -10-3 M.…”

“…4 Our current interest has concentrated on taking advantage of synthetic and/ or naturally-occurring macromolecules as a receptive component of electrochemical sensors.5 In this paper we describe a DNA-immobilized Au electrode which shows electrochemical responses to a DNA-binding drug on the basis of the redox couple-mediated "ion-channel" I11eChanlsn1. 5,6 Experimental Quinacrine was obtained from Aldrich Chemical Co. and used as received. DNA double strands were immobilized on an Au electrode via chemisorption, i.e., coordination to Au with a sulfur-containing moiety.'…”

Methods of Analysis for Chemicals that Promote/Disrupt Cellular Signaling

“…When we initiated in the late 80s some work to develop ionchannel sensors 2 and active (uphill) transport sensors [3][4][5][6] as new analytical sensors, our aim was still only to gain high sensitivity. Therefore, we even took an approach of mimicking functions of receptor ion-channel proteins and cotransporter proteins, using synthetic molecules.…”

A MutS Protein-immobilized Au Electrode for Detecting Single-base Mismatch of DNA

“…For detecting double-stranded DNA (dsDNA) containing a single nucleotide mismatch captured by the MutS immobilized on the Au-electrode surface, we took advantage of "redox couple-mediated artificial ion-channel principle" proposed by Sugawara et al 4,5 Various biosensors based on this principle have been constructed for detecting biomolecules including oligonucleotides [6][7][8][9] and ligands of receptors. [10][11][12] Using this principle, we detected dsDNA electrochemically by measuring redox peak currents on cyclic voltammogram of redox-active species.…”