Ion Channels in Renal Glomerular Mesangial Cells

Henderson, Ronald W.; Barber, Robert D.

doi:10.1007/s002329900364

Cited by 4 publications

(2 citation statements)

References 72 publications

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“…These glomeruli retraction could be at least in part induced by mesangial cell contraction (MCC). It was demonstrated that MCC could be induced by release of vasoactive hormones such as angiotensin II, that decrease the capillary surface area and consequently reducing the GFR (36–40).…”

Section: Discussionmentioning

confidence: 99%

Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats

et al. 2019

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Aim: We investigated the kidney morphofunctional consequences of high-fat diet intake since post-weaning in adult rats. Main Methods: Male Wistar rats were divided into two groups: ND (normal diet; n = 10) and HD (high-fat diet; n = 10). The high-fat diet was introduced post-weaned and animals were followed for 8 weeks. Key Findings: HD group did not change body weight gain even though food consumption has decreased with no changes in caloric consumption. The HD group showed glucose intolerance and insulin resistance. The glomerular filtration rate (GFR) was decreased in vivo (ND: 2.8 ± 1.01; HD: 1.1 ± 0.14 ml/min) and in the isolated perfusion method (34% of decrease). Renal histological analysis showed a retraction in glomeruli and an increase in kidney lipid deposition (ND: 1.5 ± 0.17 HD: 5.9 ± 0.06%). Furthermore, the high-fat diet consumption increased the pro-inflammatory cytokines IL-6 (ND: 1,276 ± 203; HD: 1,982 ± 47 pg/mL/mg) and IL-1b (ND: 97 ± 12 HD: 133 ± 5 pg/mL/mg) without changing anti-inflammatory cytokine IL-10. Significance: Our study provides evidence that high-fat diet consumption leads to renal lipid accumulation, increases inflammatory cytokines, induces glomeruli retraction, and renal dysfunction. These damages observed in the kidney could be associated with an increased risk to advanced CKD in adulthood suggesting that reduction of high-fat ingestion during an early period of life can prevent metabolic disturbances and renal lipotoxicity.

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Section: Discussionmentioning

confidence: 99%

Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats

et al. 2019

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“…It is thought that agonists that reduce the glomerular filtration rate cause mesangial cell depolarization [8,9]. This depolarization is mediated, in part, by nonselective cation channels [10].…”

Section: Introductionmentioning

confidence: 99%

Separation of two Cl– Currents in Cultured Human and Murine Mesangial Cells: Biophysical and Pharmacological Characteristics of ICl.vol and ICl.Ca

et al. 2002

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Mesangial cells, a combined smooth muscle- and fibroblast-like phenotype, are important regulators of renal function. These cells exist in a region of variable osmolarity and may require Cl^– channels for volume regulation. Additionally, Ca²⁺-activated Cl^– channels in these cells may participate in Ca²⁺-dependent contractile responses to vasoactive agonists. Relatively little, however, is known about mesangial cell Cl^– currents (I_Cl); including the biophysical description and pharmacological characterization. We used whole-cell patch clamp to study I_Cl in cultured human and SV40-transformed murine mesangial cells. I_Cl was measured in cells dialyzed and bathed with symmetrical N-methyl-D-glucamine chloride solutions to minimize cation currents. Dialysis with buffers to control intracellular Ca²⁺ ([Ca²⁺]_i), extracellular solutions of varied osmolarity, and manipulation of the transmembrane Cl^– gradient were used to separate two currents: I_Cl.vol (volume-sensitive), and I_Cl.Ca (Ca²⁺-activated). In symmetrical Cl^– with low [Ca²⁺]_i, I_Cl.vol was outwardly rectifying and modulated by osmolarity. I_Cl.vol demonstrated slight time- and voltage-dependent inactivation. In symmetrical Cl^– with elevated [Ca²⁺]_i and hypertonic bath, I_Cl.Ca was linear, but in asymmetrical Cl^– (low [Cl^–]_i) was outwardly rectifying and demonstrated time- and voltage-dependent activation. Permeability sequences for both I_Cl.vol and I_Cl.Ca were I^– > Br^– > Cl^– > F^–; however, there were differences in the relative magnitudes. Tamoxifen inhibited I_Cl.vol more potently than I_Cl.Ca, whereas niflumic acid inhibited I_Cl.Ca more potently than I_Cl.vol. We have separated and characterized two types of I_Cl in cultured human and murine mesangial cells. I_Cl.Ca and I_Cl.vol have different biophysical and pharmacological characteristics. These observations on I_Cl.Ca and I_Cl.vol may provide insight into mesangial cell reactivity and volume regulation.

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An inward rectifier and a voltage-dependent K+ current in single, cultured pericytes from bovine heart

Beckerath

Nees

Neumann

et al. 2000

Cardiovascular Research

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Ion Channels in Renal Glomerular Mesangial Cells

Cited by 4 publications

References 72 publications

Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats

Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats

Separation of two Cl<sup>–</sup> Currents in Cultured Human and Murine Mesangial Cells: Biophysical and Pharmacological Characteristics of I<sub>Cl.vol</sub> and I<sub>Cl.Ca</sub>

An inward rectifier and a voltage-dependent K+ current in single, cultured pericytes from bovine heart

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