Ion chromatography coupled to mass spectrometry as a powerful technique for halogens and sulfur determination in egg powder and its fractions

Abstract

Rationale To increase the consumption of egg powder and its fractions a suitable quality control method is required to obtain more information on its nutritional composition. The proposed method enables the quantification of important elements for the functioning of the human organism, such as halogens and sulfur, in egg powder and its fractions. Methods Up to 350 mg of egg powder or its fractions (egg white powder and egg yolk powder) were digested by microwave‐induced combustion using 20 bar pressure of oxygen. The analytes were absorbed in 100 mmol L−1 of NH4OH solution. The determination of halogens (chlorine, bromine, fluorine, and iodine) and sulfur was performed in a single analysis using ion chromatography with conductivity detection coupled to mass spectrometry. Results Using the proposed method, spike recoveries between 99% and 104% for all analytes were obtained, and results agreed with certified values of reference materials (agreements were between 100% and 109%). The relative standard deviations were below 8%. The variation in elemental concentration over a wide range in different fractions (whole egg powder, egg white powder, and egg yolk powder) and different brands was also observed. Conclusions The proposed method provides reliable information about minerals in whole egg powder and its fractions, contributing to better quality control of these products. Because these food products are widely consumed, these results suggest the safe ingestion levels of these elements.

Determination of Halogens by Ion Chromatography in Edible Mushrooms after Microwave-Induced Combustion for Sample Preparation

“…Moreover, when evaluating the pH of the diluted digests, values below 5.8 were observed for ultrapure water and 50 mmol L −1 NH 4 OH, while when 100 mmol L −1 NH 4 OH was used a pH of 7.6 was observed. It is important to point out that alkaline solutions lead to better halogen stabilization, as demonstrated in other studies [ 28 , 35 ].…”

Combining ion chromatography with mass spectrometry and inductively coupled plasma‐mass spectrometry: Annual review 2020

“…The new analytical flow path reduces the number of steps necessary, permits multicomponent analysis, and reduces the amount of chemicals. Simultaneously, the limits of detection and the sample throuput are improved compared to the AOAC International recommended methods 28,29 …”

“…Due to the high energy the compounds experience in the plasma, molecules, or multi-atom ions are cleaved, Simultaneously, the limits of detection and the sample throuput are improved compared to the AOAC International recommended methods. 28,29 It is the prebiotic value of carbohydrate fibers to support mammalian intestinal microflora's growth and balance. They control the gastrointestinal peristalsis and reduce illness incidences such as colon cancer and diarrhea.…”

“…Simultaneously, the limits of detection and the sample throuput are improved compared to the AOAC International recommended methods. 28 , 29 …”