Ion-Exchange Chromatography Coupled to Mass Spectrometry in Life Science, Environmental, and Medical Research

Ngere, Judith B; Ebrahimi, Kourosh Honarmand; Williams, Rachel; Pires, Elisabete; Walsby-Tickle, John; McCullagh, James

doi:10.1021/acs.analchem.2c04298

Cited by 26 publications

(5 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…19 Because a lot of these compounds fragment to give common ions, PO 3 (78.9) and PO 2 (62.9) the use of a triple quadrupole mass spectrometry system seemed to us to be a more appropriate detection system and such a method has been used to analyse fruits and vegetables including grapes and cereals. [20][21][22][23][24][25] For the work described here the extraction and clean-up for this method is based on a modication of the QuEChERS for polar pesticides method (QuPPE) developed by the European Union Reference Laboratory in Stuttgart and published on their website. 9 Depending on the matrix the extraction is carried out with methanol or methanol-water, in some cases acidied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A method for the quantitative analysis of polar anionic pesticides in milk/infant formula, cereals and fruit and vegetables using ion chromatography coupled to tandem mass spectrometry

Garvey,

Margalit,

Kelly

et al. 2024

Anal. Methods

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A method for the quantitative analysis of polar anionic pesticides in milk/infant formula, cereals and fruit and vegetables using ion chromatography coupled to tandem mass spectrometry

Garvey,

Margalit,

Kelly

et al. 2024

Anal. Methods

View full text Add to dashboard Cite

show abstract

“…The ICP-based methods are highly sensitive, but they can only be deployed to measure cation activities. Accordingly, activities of anions in coexisting phases were measured using ion selective electrodes (ISE) 29 and ion chromatography (IC) 30 ( Figure 1D ).…”

Section: Introductionmentioning

confidence: 99%

Biomolecular condensates are characterized by interphase electric potentials

Posey,

Bremer,

Erkamp

et al. 2024

Preprint

View full text Add to dashboard Cite

Biomolecular condensates form via processes that combine phase separation and reversible associations of multivalent macromolecules. Condensates can be two- or multi-phase systems defined by coexisting dense and dilute phases. Here, we show that solution ions can partition asymmetrically across coexisting phases defined by condensates formed by intrinsically disordered proteins or homopolymeric RNA molecules. Our findings were enabled by direct measurements of the activities of cations and anions within coexisting phases of protein and RNA condensates. Asymmetries in ion partitioning between coexisting phases vary with protein sequence, condensate type, salt concentration, and ion type. The Donnan equilibrium set up by asymmetrical partitioning of solution ions generates interphase electric potentials known as Donnan and Nernst potentials. Our measurements show that the interphase potentials of condensates are of the same order of magnitude as membrane potentials of membrane-bound organelles. Interphase potentials quantify the degree to which microenvironments of coexisting phases are different from one another. Importantly, and based on condensate-specific interphase electric potentials, which are membrane-like potentials of membraneless bodies, we reason that condensates are mesoscale capacitors that store charge. Interphase potentials lead to electric double layers at condensate interfaces. This helps explain recent observations of condensate interfaces being electrochemically active.

show abstract

“…However, there are several challenges to effectively characterizing these compounds using LC-MS. Because of their high polarity, native (i.e., underivatized or untagged) organic acids have been separated using HILIC [6,7], but this approach can require lengthy column reequilibration steps between injections [8,9]. Ion-exchange chromatography has also been used for the separation of organic acids, although challenges exist when coupling this technique to MS [10]. A recent review detailed such methods used for the analysis of these compounds in biological samples by LC [11].…”

Section: Introductionmentioning

confidence: 99%

Use of N‐(4‐aminophenyl)piperidine derivatization to improve organic acid detection with supercritical fluid chromatography‐mass spectrometry

Saw,

Boughton,

Wroniuk

et al. 2023

J of Separation Science

View full text Add to dashboard Cite

The analysis of organic acids in complex mixtures by LC‐MS can often prove challenging, especially due to the poor sensitivity of negative ionization mode required for detection of these compounds in their native (i.e., underivatized or untagged) form. These compounds have also been difficult to measure using supercritical fluid chromatography (SFC)‐MS, a technique of growing importance for metabolomic analysis, with similar limitations based on negative ionization. In this report, the use of a high proton affinity N‐(4‐aminophenyl)piperidine derivatization tag is explored for the improvement of organic acid detection by SFC‐MS. Four organic acids (lactic, succinic, malic, and citric acids) with varying numbers of carboxylate groups were derivatized with N‐(4‐aminophenyl)piperidine to achieve detection limits down to 0.5 ppb, with overall improvements in detection limit ranging from 25‐to‐2100‐fold. The effect of the derivatization group on sensitivity, which increased by at least 200‐fold for compounds that were detectable in their native form, and mass spectrometric detection are also described. Preliminary investigations into the separation of these derivatized compounds identified multiple stationary phases that could be used for complete separation of all four compounds by SFC. This derivatization technique provides an improved approach for the analysis of organic acids by SFC‐MS, especially for those that are undetectable in their native form.

show abstract

Ion-Exchange Chromatography Coupled to Mass Spectrometry in Life Science, Environmental, and Medical Research

Cited by 26 publications

References 152 publications

A method for the quantitative analysis of polar anionic pesticides in milk/infant formula, cereals and fruit and vegetables using ion chromatography coupled to tandem mass spectrometry

A method for the quantitative analysis of polar anionic pesticides in milk/infant formula, cereals and fruit and vegetables using ion chromatography coupled to tandem mass spectrometry

Biomolecular condensates are characterized by interphase electric potentials

Use of N‐(4‐aminophenyl)piperidine derivatization to improve organic acid detection with supercritical fluid chromatography‐mass spectrometry

Contact Info

Product

Resources

About