Ion implantation induced nanotopography on titanium and bone cell adhesion

Braceras, I.; Vera, Carolina; Ayerdi-Izquierdo, Ana; Vázquez-Muñoz, Roberto; Lorenzo, Jaione; Álvarez, Noelia; Maeztu, M.A. De

doi:10.1016/j.apsusc.2014.03.118

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…By applying controlled surface topography, processes including the kinetics and force of cellular adhesion to a surface, migration, proliferation, and differentiation of cells, and deposition of extracellular matrix proteins and minerals might all be obtained. − On the basis of the importance of surface nanotopography, nanofabrication techniques were introduced to modify the surface properties of bone biomaterials and enhance functional outcomes. A number of studies have investigated the behaviors of bone cells, especially osteoblastic lineage cells, on differing nanotopographies. ,− The adhesion, migration, proliferation, and differentiation of osteoblastic cells were successfully regulated by modifying nanotopography. ,− The mechanisms by which nanotopography mediates osteogenesis may involve the coactivation of adhesion and bone morphogenic protein signaling pathways in mesenchymal stromal cells . In addition to its effects on bone cells, nanotopography also has a significant effect on immune cells.…”

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confidence: 99%

Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications

et al. 2017

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Osteoimmunomodulation has informed the importance of modulating a favorable osteoimmune environment for successful materials-mediated bone regeneration. Nanotopography is regarded as a valuable strategy for developing advanced bone materials, due to its positive effects on enhancing osteogenic differentiation. In addition to this direct effect on osteoblastic lineage cells, nanotopography also plays a vital role in regulating immune responses, which makes it possible to utilize its immunomodulatory properties to create a favorable osteoimmune environment. Therefore, the aim of this study was to advance the applications of nanotopography with respect to its osteoimmunomodulatory properties, aiming to shed further light on this field. We found that tuning the surface chemistry (amine or acrylic acid) and scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome. This study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a "nanoengineered surface" for the development of advanced bone biomaterials with favorable osteoimmunomodulatory properties.

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confidence: 99%

Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications

et al. 2017

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“…It is known that surface topography plays an important role in the regulation of cell behaviors and functions. The adhesion, migration, proliferation, and differentiation of osteoblastic cells can be successfully regulated by fabricating nanotopography, − since biomaterials with 3D nanofibrous architectures guide the morphology of natural extracellular matrix (ECM) at the nano level, , not only effectively upregulating the expression of integrins − but also presenting more binding sites to cell membrane receptors . In many bone-tissue-engineering strategies, a fibrous network is used as a template for subsequent mineralization in a similar way to collagen fibers in natural bone remodeling, and the fabricated matrix forms a bonelike structure and provides an attractive microenvironment for osteogenic function in resident cells. , In addition, injectable self-assembling nanofibrous networks introduced to injury sites have been used in vivo to provide a 3D structural environment that has been shown to increase the infiltration and retention of endogenous cells. , In a previous study, we characterized the surface topography of osteoclast-resorbed human bone surfaces using four synergistic parameters (fractal dimension, lacunarity, porosity, and surface roughness) and compared the synergistic parameters with those obtained from two different groups of Ti implant surfaces.…”

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confidence: 99%

Bioinspired Fabrication of Calcium-Doped TiP Coating with Nanofibrous Microstructure to Accelerate Osseointegration

et al. 2020

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Bioinspired by the morphology of osteoclast-resorbed bone surfaces, we prepared a calcium-doped titanium phosphate (Ca-TiP) coating, which consists of a nanofibrous network, on titanium (Ti) substrate via a simple two-step hydrothermal method, trying to mimic natural bone compositionally and microstructurally. The in vitro studies show that the Ca-TiP coating with synergistic features of nanofibrous biomimetic topography and surface chemistry could elicit intensively osteogenic behavior and responses including enhanced cell adhesion, spreading, and proliferation as well as alkaline phosphatase (ALP) activity and up-regulated expression of bone-related genes, which inevitably benefit the formation of new bone and the quality of osseointegration. When the two control groups are compared in vivo, the significantly improved new bone formation in the early stage and the much stronger interfacial bonding with the surrounding bone for Ca-TiP coating suggest that Ca-TiP coating modified Ti implants hold great potential for orthopedic and dental applications.

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“…Therefore, it is essential to optimize the biological and mechanical properties of a metallic implant to obtain an ideal bioimplant surface. 41 Although plasma spray is a state-of-The-art technique for surface modification of Ti implants, it has several disadvantages that include various phases of calcium phosphate in the coating, giving rise to surface delamination and changes in the crystal structure of the matrix, subsequently releasing the coated nanoparticles that may cause inflammatory reactions. 42 To overcome these drawbacks of plasma spray, the conventional ion implantation technique has been utilized in the present study, which is supposed to unalter the nano-topography patterned on the Ti surface without changing its surface chemistry.…”

Section: Discussionmentioning

confidence: 99%

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

Swain,

Pradhan,

Bhuyan

et al. 2024

IJN

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There is an ongoing need for improved healing response and expedited osseointegration on the Ti implants in acetabular fracture sites. To achieve adequate bonding and mechanical stability between the implant surface and the acetabular fracture, a new coating technology must be developed to promote bone integration and prevent bacterial growth. Methods: A cylindrical Ti substrate mounted on a rotating specimen holder was used to implant Ca 2+ , P 2+ , and Sr 2+ ions at energies of 100 KeV, 75 KeV and 180 KeV, respectively, using a low-energy accelerator to synthesize strontium-substituted hydroxyapatite at varying conditions. Ag 2+ ions of energy 100 KeV were subsequently implanted on the as-formed surface at the near-surface region to provide anti-bacterial properties to the as-formed specimen. Results:The properties of the as-formed ion-implanted specimen were compared with the SrHA-Ag synthesized specimens by cathodic deposition and low-temperature high-speed collision technique. The adhesion strength of the ion-implanted specimen was 43 ± 2.3 MPa, which is well above the ASTM standard for Ca-P coating on Ti. Live/dead cell analysis showed higher osteoblast activity on the ion-implanted specimen than the other two. Ag in the SrHA implanted Ti by ion implantation process showed superior antibacterial activity. Discussion: In the ion implantation technique, nano-topography patterned surfaces are not concealed after implantation, and their efficacy in interacting with the osteoblasts is retained. Although all three studies examined the antibacterial effects of Ag 2+ ions and the ability to promote bone tissue formation by MC3T3-E1 cells on SrHA-Ag/Ti surfaces, ion implantation techniques demonstrated superior ability. The synthesized specimen can be used as an effective implant in acetabular fracture sites based on their mechanical and biological properties.

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Ion implantation induced nanotopography on titanium and bone cell adhesion

Cited by 17 publications

References 33 publications

Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications

Tuning Chemistry and Topography of Nanoengineered Surfaces to Manipulate Immune Response for Bone Regeneration Applications

Bioinspired Fabrication of Calcium-Doped TiP Coating with Nanofibrous Microstructure to Accelerate Osseointegration

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

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