1996
DOI: 10.1021/ja960495l
|View full text |Cite
|
Sign up to set email alerts
|

Ionic Effects on the Stability and Conformation of Peptide Nucleic Acid Complexes

Abstract: Peptide nucleic acid (PNA) is a DNA analogue in which the negatively charged sugar phosphate backbone has been substituted by uncharged N-(2-aminoethyl)glycine units. The study of a PNA−DNA duplex and the corresponding DNA−DNA duplex gives a unique opportunity to compare two polyelectrolytes with virtually identical geometry but greatly different linear charge density. The results provide a basis for a study of the applicability of the Poisson−Boltzmann (PB) and counterion condensation (CC) theories. UV and ci… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4

Citation Types

41
269
1
1

Year Published

1998
1998
2005
2005

Publication Types

Select...
8
1

Relationship

2
7

Authors

Journals

citations
Cited by 274 publications
(312 citation statements)
references
References 40 publications
(80 reference statements)
41
269
1
1
Order By: Relevance
“…We also noticed a similar although less pronounced minimum (Ϸ7% hypochromicity) on the room temperature-mixing curve for pcPNAs 1495͞1496 (data not shown), which were studied earlier (9). A weak hypochromic effect exhibited by pcPNA duplexes, which is smaller than usually observed with common PNA duplexes (33), is one of the reasons why this effect was previously overlooked (9). Fig.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…We also noticed a similar although less pronounced minimum (Ϸ7% hypochromicity) on the room temperature-mixing curve for pcPNAs 1495͞1496 (data not shown), which were studied earlier (9). A weak hypochromic effect exhibited by pcPNA duplexes, which is smaller than usually observed with common PNA duplexes (33), is one of the reasons why this effect was previously overlooked (9). Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The temperature dependencies of the fraction of melted pcPNA duplexes, f, were analyzed to estimate the integral enthalpy (⌬H) of pcPNA duplex melting͞formation. Considering the melting of these duplexes as the all-or-non transition, the values of the equilibrium constant of duplex formation, K d , were determined at each temperature by using the equation (27,33):…”
Section: Resultsmentioning
confidence: 99%
“…Furthermore, it should be noted that PNA oligonucleotides are excellent candidates for SNP detection probes because the PNA͞DNA duplexes formed can be nearly twice as destabilized by mismatches, relative to natural DNA͞DNA complexes (41). The PNA͞DNA duplexes are also much less sensitive to the ionic strength of the solution (42).…”
Section: Resultsmentioning
confidence: 99%
“…The theoretical and biochemical basis for PNA interaction with nucleic acids has been described in detail elsewhere (18,29,49). Our general interest in PNA stems from studies indicating that PNA oligos will bind as well as or better than DNA oligos under identical solution conditions (ionic strength or temperature).…”
Section: Resultsmentioning
confidence: 99%